Abstract
To introduce useful genes from the wild species Thinopyrum intermedium into cultivated wheat, a wheat-Th. intermedium disomic addition line (TAI27) was used as source material for tissue culture. TAI27 contains, beside the 42 wheat chromosomes, a pair of smaller chromosomes that is cytologically discernible. Based on restriction fragment length polymorphism (RFLP) analysis, this chromosome pair was determined to be a recombinant one, comprising segments with homoeology to at least two chromosome groups of wheat, i.e., group 2 and 7. Sixty-eight green plants were regenerated from six month-old embryogenic calli initiated from immature embryos of TAI27. Four of the plants were found to have only 42 cytologically normal-looking chromosomes. Southern blot analysis using a Th. intermedium-enriched repetitive probe showed that one of the plants had hybridization fragments specific to Th. intermedium, indicating introgression of chromatin during the cell culture process.
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References
Banks PM, Larkin PJ, Bariana HS, Lagudah ES, Appels R, Waterhouse PM, Brettell RIS, Chen X, Xu HJ, Xin ZY, Qian YT, Zhou XM, Cheng ZM & Zhou GH (1995) The use of cell culture for subchromosomal introgression of barley yellow dwarf virus resistance from Thinopyrum intermedium to wheat. Genome 38: 395-405
Feinberg AP & Vogelstein B (1983) A technique for radio-labeling DNA restriction fragments to a high specific activity. Anal. Biochem. 132: 6-13
Gale MD, Atkinson MD, Chinoy CN, Harcourt RL, Jia J, Li QY & Devos KM (1993) Genetic maps of hexaploid wheat. In: Li ZS & Xin ZY (eds). Proc. 8th Int. Wheat Genet. Symp. (pp 29-40). China Agricultural Scientech Press, Beijing
He MY, Xu ZY, Zou MQ, Zhang H, Piao ZS & Hao S (1988). The establishment of two sets of alien addition lines of wheat-wheatgrass. Sci. Sin. (Series B) 32: 695-705
Kidwell KK & Osborn TC (1992) Simple plant DNA isolation procedures. In: Beckmann JS & Osborn TC (eds). (pp 1-3) Plant Genomes: Methods for Genetic and Physical Mapping. Kluwer Academic Publishers, Dordrecht, The Netherlands
Larkin PJ, Banks PM, Lagudah ES, Appels R, Chen X, Xin ZY, Ohm HW & McIntosh R (1995) Disomic Thinopyrum intermedium addition lines in wheat with barley dwarf virus resistance and with rust resistance. Genome 38: 385-394
Lapitan NLV, Sears RG & Gill BS (1984) Translocations and other karyotypic structural changes in wheat x rye hybrids regenerated from tissue culture. Theor. Appl. Genet. 68: 547-554
Liu B, Sega LG, Vega JM, Feldman M & Abbo S (1997) Isolation and characterization of chromosome-specific DNA sequences from a chromosome arm-specific library of common wheat. The Plant Journal 11: 959-965
Wu QS, Liu B & Liu DJ (1993) Wheat protoplast culture and morphogenesis. J. Nanjing Agri. Univ. 16: 1-6
Zhang XQ, Chen DW, Bu XL, He MY & Hao S (1991) Variation of Thinopyrum intermedium chromosomes in wheat-Th. intermedium alien addition lines. Acta Genet. Sin. 18: 344-351
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Liu, B., Luan, Y., Han, F. et al. Cell culture induced introgression of Thinopyrum intermedium chromatin into common wheat. Plant Cell, Tissue and Organ Culture 65, 9–13 (2001). https://doi.org/10.1023/A:1010610325332
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DOI: https://doi.org/10.1023/A:1010610325332