Abstract
The lipolytic activity of cutinase produced by a recombinant Saccharomyces cerevisiae strain was compared with Defat 50, a commercial lipase solution, for their degreasing abilities on a lipidic concentrate of sheep fleshing, a solid by-product of the leather industry. The specific activities obtained were 2749 and 938 U mg−1 of lipase for cutinase and Defat 50, respectively.
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Blum H, Hildburg B, Gross H (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Elecrophoresis 8: 93–99.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Christner J (1992) The use of lipases in the beamhouse processes. J. Amer. Leather Chem. Assoc. 87: 128–139.
Cistola DP, Hamilton JA, Jackson D, Small DM (1988) Ionization and phase behaviour in water: application of the Gibbs phase rule. Biochemistry 27: 1881–1888.
Cistola DP, Small DM, Hamilton JA (1982) Ionization behaviour of aqueous short chain carboxylic acids: a carbon-13 NMR study. J. Lipid Res. 23: 795–799.
Dalev PG, Simeonova LS (1992) An enzyme biotechnology for the total utilization of leather wastes. Biotechnol. Lett. 14: 531–534.
Delvaux A, Lemos M, Moreau C, Erneux C (1990) Regeneration of enzymatic activity after sodium dodecylsulfate/polyacrylamide gel electrophoresis and zinc acetate staining: the example of inositol 1,4,5 triphosphate 5-phosphatase. Anal. Biochem. 188: 219–221.
Egmond MR, de Vlieg J, Verhey HM, de Haas GH (1996) Strategies and design of mutations in Lipases. In: Malcata FX, ed. Engineering of/with Lipases. Dordrecht: Kluwer Academic Publishers, pp. 193–202.
Flipsen JAC, Appel ACM, van der Hijden HTWM, Verrips CT (1998) Mechanism of removal of immobilized triacylglycerol by lipolytic enzymes in a sequential laundry wash process. Enzyme Microbiol. Technol. 23: 274–280.
Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497–509.
Jacobsen T, Olsen J, Allermann K (1989) Production, partial purifi-cation and immunochemical characterization of multiple forms of lipases from Geotrichum candidum. Enzyme Microbiol. Technol. 11: 90–95.
Kolattukudy PE (1984) Cutinases from fungi and pollen. In: Borgström B, Brockman H, eds. Lipases. Amsterdam: Elsevier, pp. 471–504.
Lauwereys M, DeGeus P, DeMeutter J, Stanssens P, Matthyssens G (1991) Cloning, expression and characterisation of cutinase, a fungal lipolytic enzyme. In: Alberghina L, Schmid RD, Verger R, eds. Lipases: Structure, Mechanism and Genetic Engineering, Vol. 16. Braunschweig: Gesellshaft für Biotechnologische Forschung Monographs, pp. 243–251.
McGrew BR, Green MD (1990) Enhanced removal of detergent and recovery of enzymatic activity following dodecyl sulfatepolyacrylamide gel electrophoresis: use of casein in gel wash buffer. Anal. Biochem. 189: 68–74.
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
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Teles, F.R., Cabral, J.M. & Santos, J.A. Enzymatic degreasing of a solid waste from the leather industry by lipases. Biotechnology Letters 23, 1159–1163 (2001). https://doi.org/10.1023/A:1010596206857
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DOI: https://doi.org/10.1023/A:1010596206857