Skip to main content
Log in

On-line determination of flocculating Saccharomyces cerevisiae concentration and growth rate using a capacitance probe

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

During continuous alcoholic fermentation, Saccharomyces cerevisiae 38A floc concentration was monitored using an on-line impedance probe. Since the sensor response is linear and does not depend significantly on yeast particle size, an automatic technique of determining the yeast growth rate has been developed and validated against the conventional mass balance method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bu'Lock JD, Comberbach DM, Ghommidh C (1984a) A study of continuous ethanol production using a highly flocculent yeast in the gas-lift tower fermenter. Chem. Eng. J. 29: 9–24.

    Google Scholar 

  • Bu'Lock JD, Comberbach DM, Ghommidh C, Williams PD (1984b) A computer controlled fermentation operating system for highlevel alcohol production. Chem. Ind. 18: 432–434.

    Google Scholar 

  • Comberbach DM, Bu'Lock JD (1983) Automatic on-line fermentation headspace gas analysis using a computer controlled gas chromatograph. Biotechnol. Bioeng. 25: 2503–2518.

    Google Scholar 

  • Davey CL, Davey HM, Kell DB (1992) On the dielectric properties of cell suspensions at high volume fractions. Bioelectrochem. Bioenerg. 28: 319–340.

    Google Scholar 

  • Fontana A, Ghommidh C, Guiraud JP, Navarro JM (1992) Continuous alcoholic fermentation of sucrose using flocculating yeast: the limits of invertase activity. Biotechnol. Lett. 14: 505–510.

    Google Scholar 

  • Kell DB, Samworth CM, Todd RW, Bungard SJ, Morris GJ (1987) Real-time estimation of microbial biomass during fermentations, using a dielectric probe. Studia Biophysica 119: 153–156.

    Google Scholar 

  • Kida K, Yamadaki M, Asano S, Nakata T, Sonoda Y (1989) The effect of aeration on stability of continuous ethanol fermentation by a flocculating yeast. J. Ferm. Bioeng. 68: 107–111.

    Google Scholar 

  • Markx GH, Davey CL, Kell DB (1991) The permittistat: a novel type of turbidostat. J. Gen. Microbiol. 137: 735–743.

    Google Scholar 

  • Mas S, Ghommidh C (2001) On-line sizing of yeast aggregates using image analysis. Biotechnol. Bioeng. (in press).

  • Mishima K, Mimura A, Takahara Y (1991) On-line monitoring of cell concentrations during yeast cultivation by dielectric measurements. J. Ferment. Bioeng. 72: 296–299.

    Google Scholar 

  • Penaloza W, Davey CL, Hedger JN, Kell DB (1992) Physiological studies on the solid-state quinoa tempe fermentation, using online measurements of fungal biomass production. J. Sci. Food. Agric. 59: 227–235.

    Google Scholar 

  • Schwan HP (1957) Electrical properties of tissue and cell suspensions. Adv. Biol. Med. Phys. 5: 147–209.

    Google Scholar 

  • Wieczorek A, Michalski H (1994) Continuous ethanol production by flocculating yeast in the fluidized bed bioreactor. FEMS Microbiol. Rev. 14: 69–74.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to C. Ghommidh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mas, S., Ossard, F. & Ghommidh, C. On-line determination of flocculating Saccharomyces cerevisiae concentration and growth rate using a capacitance probe. Biotechnology Letters 23, 1125–1129 (2001). https://doi.org/10.1023/A:1010563917374

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1010563917374

Navigation