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Enantioselective production of levofloxacin by immobilized porcine liver esterase

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Abstract

Porcine liver esterase, which cleaves ofloxacin butyl ester enantioselectively to levofloxacin, was successfully immobilized in calcium alginate and polyacrylamide gel. Immobilized esterase in 5% (w/v) calcium alginate exhibited 58% immobilization efficiency and could be reused five times without severe loss of enzyme activity. On the other hand, entrapped esterase in polyacrylamide gel, composed of 20% of total monomer and 8.3% of cross-linking agent, could be reused 10 times, and 51% of enzyme activity remained after the 10th batch without decrease of enantioselectivity. Compared with entrapped methods, significant reduction of enzyme activity was found in the case of physical adsorption on to QAE-Sephadex.

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References

  • Cao SG, Yang H, Ma L, Guo SQ (1996) Enhancing enzymatic properties by the immobilization method. Appl. Biochem. Biotechnol. 59: 7-14.

    Google Scholar 

  • Das N, Kayastha AM, Malhotra OP (1998) Immobilization of urease from pigeonpea (Cajanus cajan L.) in polyacrylamide gels and calcium alginate beads. Biotechnol. Appl. Biochem. 27: 25-29.

    Google Scholar 

  • Fernandez-Lafuente R, Cowan DA, Wood ANP (1995) Hyper-stabilization of a thermophilic esterase by multipoint covalent attachment. Enzyme Microbiol. Tech. 17: 366-372

    Google Scholar 

  • Goncalves APV, Cabral JMS, Aires-Barros MR (1996) Immobilization of a recombinant curtinase by entrapment and by covalent binding: kinetic and stability studies. Appl. Biochem. Biotechnol. 60: 217-228.

    Google Scholar 

  • Kim BY, Hwang KC, Song HS, Chung NH, Bang WK (2000) Optical resolution of RS(±)-mandelic acid by Pseudomonas sp. Biotechnol. Lett. 22: 1871-1875.

    Google Scholar 

  • Kim MG, Lee SB (1996) Enzymatic resolution of racemic ibuprofen by lipase-catalyzed esterification reaction: effects of water content and solid supports. J. Ferment. Bioeng. 81: 269-271.

    Google Scholar 

  • Lee SY, Min BH, Hwang SH, Koo YM, Lee CG, Song SW, Oh SY, Lim SM, Kim SL, Kim DI (2000) Enantioselective production of levofloxacin from ofloxacin butyl ester by porcine liver esterase. Kor. J. Biotechnol. Bioeng. 15: 313-317.

    Google Scholar 

  • Liu BL, Jong CH, Tzeng YM (1999) Effect of immobilization on pH and thermal stability of Aspergillus ficuum phytase. Enzyme Microbiol. Tech. 25: 517-521.

    Google Scholar 

  • Martinez-Martinez L, Pascual A, Suarez AI, Perea EJ (1999) In-vitro activity of levofloxacin, ofloxacin and D-ofloxacin against coryneform bacteria and Listeria monocytogenes. J. Antimicrobiol. Chemother. 43: 27-32.

    Google Scholar 

  • Martinsen A, Skjåk-Bræk G, Smidsrød O (1989) Alginate as immobilization material: I. Correlation between chemical and physical properties of alginate gel beads. Biotechnol. Bioeng. 33: 79-89.

    Google Scholar 

  • Martinsen A, Storrø I, Skjåk-Bræk G (1992) Alginate as immobilization material: III. Diffusional properties. Biotechnol. Bioeng. 39: 186-194.

    Google Scholar 

  • Palmer T (1995) Understanding Enzymes, 4th edn. New York: Practice Hall/Ellis Horwood.

    Google Scholar 

  • Pizarro C, Fernandez-Torroba MA, Benito C, Gonzalez-Saiz JM (1997) Optimization by experimental design of polyacrylamide gel composition as support for enzyme immobilization by entrapment. Biotechnol. Bioeng. 53: 497-506.

    Google Scholar 

  • Puri M, Marwaha SS, Kothari RM (1996) Studies on the applicability of alginate-entrapped naringinase debittering of kinnow juice. Enzyme Microbiol. Tech. 18: 281-285.

    Google Scholar 

  • Tanaka H, Matsumura M, Veliky IA (1984) Diffusion characteristics of substrates in Ca-alginate gel beads. Biotechnol. Bioeng. 26: 53-58.

    Google Scholar 

  • Tischer W, Wedekind F (1999) Immobilized enzymes: methods and applications. Topics Curr. Chem. 200: 95-126.

    Google Scholar 

  • Vicenzi JT, Zmijewski MJ, Reinhard MR, Landen BE, Muth WL, Marler PG (1997) Large-scale stereoselective enzymatic ketone reduction with in situ product removal via polymeric adsorbent resins. Enzyme Microbiol. Tech. 20: 494-499.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Sang-Yoon Lee, Byung-Hyuk Min, Sung-Ho Hwang, Yoon-Mo Koo, Choul-Kyun Lee & Dong-II Kim

  2. Central Research Institute, Boryung Pharmaceutical Co., Ansan, 425-120, Korea

    Seong-Won Song, Sun-Young Oh, Sang-Min Lim & Sang-Lin Kim

Authors
  1. Sang-Yoon Lee
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  2. Byung-Hyuk Min
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  3. Sung-Ho Hwang
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  4. Yoon-Mo Koo
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  5. Choul-Kyun Lee
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  6. Seong-Won Song
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  7. Sun-Young Oh
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  8. Sang-Min Lim
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  9. Sang-Lin Kim
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  10. Dong-II Kim
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Correspondence to Dong-II Kim.

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Lee, SY., Min, BH., Hwang, SH. et al. Enantioselective production of levofloxacin by immobilized porcine liver esterase. Biotechnology Letters 23, 1033–1037 (2001). https://doi.org/10.1023/A:1010554118301

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  • DOI: https://doi.org/10.1023/A:1010554118301

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