Kinetic model of lysozyme renaturation with the molecular chaperone GroEL

Abstract

From the renaturation kinetics of denatured/reduced lysozyme assisted by the molecular chaperone GroEL, a simplified kinetic model was established based on the competition between protein folding and aggregation. In the presence of GroEL and ATP, the aggregate formation was a second order reaction. With 2 mM ATP, a renaturation yield of 90% at a high renaturation rate was obtained when the molar ratio of GroEL to lysozyme was 1:1.

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Correspondence to Yan Sun.

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Dong, XY., Wang, YB., Liu, XG. et al. Kinetic model of lysozyme renaturation with the molecular chaperone GroEL. Biotechnology Letters 23, 1165–1169 (2001). https://doi.org/10.1023/A:1010504824604

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  • GroEL
  • kinetics
  • lysozyme
  • model
  • renaturation