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A gene encoding Achlya bisexualis β-amylase and its expression in Saccharomyces cerevisiae

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Abstract

Part of a β-amylase genomic DNA sequence from the oomycete, Achlya bisexualis was cloned by polymerase chain reaction (PCR) using degenerate oligonucleotide primers derived from the conserved regions of other known β-amylase sequences. The 5′- and 3′-regions of the β-amylase gene were amplified by genome walking method. The Ach. bisexualis β-amylase gene consisted of a 1338bp open reading frame, encoding a protein of 446 amino acids with a molecular weight of 49 381Da, and was not interrupted by any intron. The deduced amino acid sequence of the β-amylase gene had 67% similarity to the β-amylase of Saprolegnia ferax, followed by 40% similarity to that ofArabidopsis thaliana. The β-amylase gene was expressed in Saccharomyces cerevisiae placing it under the control of the alcohol dehydrogenase gene (ADC1) promoter.

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Authors and Affiliations

  1. Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University, Kwangju, 500-757, Korea

    Hee-Ok Kim, Jeong-Nam Park, Dong-Jun Shin, Hwanghee Blaise Lee, Soon-Bai Chun & Suk Bai

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  1. Hee-Ok Kim
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  2. Jeong-Nam Park
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  3. Dong-Jun Shin
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  4. Hwanghee Blaise Lee
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  5. Soon-Bai Chun
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  6. Suk Bai
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Correspondence to Suk Bai.

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Kim, HO., Park, JN., Shin, DJ. et al. A gene encoding Achlya bisexualis β-amylase and its expression in Saccharomyces cerevisiae. Biotechnology Letters 23, 1101–1107 (2001). https://doi.org/10.1023/A:1010503731486

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