Abstract
Recombinant rotavirus VP6 production was 0.49 mg l−1 when 10 mM sodium butyrate was added to the suspension culture of transgenic tomato cells after 6 days' incubation. Recombinant VP6 production increased to 0.73 mg l−1, when the culture was supplemented with 10 mM sodium butyrate on day 6 and 9 after incubation. Total protein extracts of transgenic tomato cells from sodium butyrate-supplemented culture were examined by two-dimensional electrophoresis: 14 proteins were up-regulated and 7 down-regulated.
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Arts J, Lansink M, Grimbergen J, Toet KH, Kooistra T (1995) Stimulation of tissue-type plasminogen activator gene expression by sodium butyrate and trichostatin A in human endothelial cells involves histone acetylation. Biochem. J. 310: 171-176.
Chung IS, Kim CH, Kim KI, Hong SH, Park JH, Kim JK, Kim WY (2000) Production of recombinant rotavirus VP6 from a suspension culture of transgenic tomato (Lycopersicon esculentum Mill.) cells. Biotechnol. Lett. 22: 251-255.
Cuisset L, Tichonicky L, Jaffray P, Delpech M (1997) The effects of sodium butyrate on transcription are mediated through activation of a protein phosphatase. J. Biol. Chem. 272: 24148-24153.
Dameval C, De Vienne D, Zivy M, Thiellement H (1986) Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling protein. Electrophoresis 7: 53-54.
Dorner AJ, Wasley LC, Kaufman RJ (1989) Increased synthesis of secreted proteins induces expression of glucose-regulated proteins in butyrate-treated Chinese hamster ovary cells. J. Biol. Chem. 264: 20602-20607.
Oh SKW, Vig P, Chua F, Teo WK, Yap MGS (1993) Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate. Biotechnol. Bioeng. 42: 601-610.
Oster T, Thioudellet C, Chevalot I, Masson C, Wellman M, Marc A, Siest G (1993) Induction of recombinant human gammaglutamyl transferase by sodium butyrate in transfected V79 and CHO Chinese hamster cells. Biochem. Biophys. Res. Commun. 193: 406-412.
Pedersen TJ, Minocha SC (1988) Effect of n-sodium butyrate on cell division in Jerusalem artichoke (Helianthus tuberosus L.) tuber explants cultured in vitro. J. Plant Physiol. 132: 623-630.
Rocchi P, Ferreri AM, Simone G, Granchi D, Paolucci P, Paolucci G (1992) Growth inhibitory and differentiating effects of sodium butyrate on human neuroblastoma cells in culture. Anticancer Res. 12: 917-920.
Saito H, Kagawa T, Tada S, Tsunematsu S, Guevara FM, Watanabe T, Morizane T, Tsuchiya M (1992) Effect of dexamethasone, dimethylsulfoxide and sodium butyrate on a human hepatoma cell line PLC/PRF/5. Cancer Biochem. Biophys. 13: 75-84.
Shadan FF, Cowsert LM, Villarreal LP (1994) n-Butyrate, a cell cycle blocker, inhibits the replication of polyomaviruses and papillomaviruses but not that of adenoviruses and herpesviruses. J. Virol. 68: 4785-4796.
Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels. Anal. Chem. 68: 850-858.
Yamamoto I, Matsunaga T, Sakata K, Nakamura Y, Doi S, Hanmyou F (1996) Histone hyperacetylation plays a role in augmentation of IL-4-induced IgE production in LPS-stimulated murine B-lymphocytes by sodium butyrate. J. Biochem. 119: 1056-1061.
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Kim, C.H., Il Kim, K., Hyun Hong, S. et al. Improved production of recombinant rotavirus VP6 in sodium butyrate-supplemented suspension cultures of transgenic tomato (Lycopersicon esculentum Mill.) cells. Biotechnology Letters 23, 1061–1066 (2001). https://doi.org/10.1023/A:1010502322496
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DOI: https://doi.org/10.1023/A:1010502322496