Abstract
The analysis of sixteen Komagataea (Williopsis) pratensisisolates from Caucasian and Tien Shan soils by the PCR, blot hybridization, and isoenzyme electrophoresis techniques showed that fifteen of them do belong to the species K. pratensis. The isolates from the two geographic areas differed in some physiological characteristics and in the PCR product profiles obtained with the microsatellite primers (CAC)5and (GACA)4.
Similar content being viewed by others
References
Bab'eva, I.P. and Reshetova, I.S., A New Species of Soil Yeasts, Williopsis pratensis, Mikrobiologiya, 1979, vol. 48, pp. 1040–1043.
Yamada, Y., Matsuda, M., Maeda, K., Sakakibara, C., and Mikata, K., The Phylogenetic Relationships of the Saturn-shaped Ascospore-forming Species of the Genus Williopsis Zender and Related Genera Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae): the Proposal of Komagataea gen. nov., Biosci. Biotechnol. Biochem., 1994, vol. 58, no. 7, pp. 1236–1244.
James, S.A., Roberts, I.N., and Collins, M.D., Phylogenetic Heterogeneity of the Genus Williopsis as Revealed by 18S rRNA Gene Sequences, Int. J. Syst. Bacteriol., 1998, vol. 48, pp. 591–596.
Liu, Z. and Kurtzman, C.P., Phylogenetic Relationships among Species of Williopsis and Saturnospora gen. nov. as Determined from Partial rRNA Sequences, Antonie van Leeuwenhoek, 1991, vol. 60, pp. 21–30.
Drissi, R., Sor, F., Nosek, J., and Fukuhara, H., Genes of the Linear Mitochondrial DNA of Williopsis mrakii: Coding Sequences for a Maturase-Like Protein, a Ribosomal Protein VAR1 Homologue, Cytochrome Oxidase Subunit 2 and Methionyl tRNA, Yeast, 1994, vol. 10, pp. 391–398.
Kurtzman, C.P., DNA Relatedness among Saturn-spored Yeasts Assigned to the Genera Williopsis and Pichia, Antonie van Leeuwenhoek, 1991, vol. 60, pp. 13–19.
The Yeasts: A Taxonomic Study, Kurtzman, C.P. and Fell, J.W., Eds., Elsevier, 1998.
Lieckfeldt, E., Meyer, W., and Borner, T., Rapid Identifi-cation and Differentiation of Yeasts by DNA and PCR Fingerprinting, J. Basic Microbiol., 1993, vol. 33, no. 6, pp. 413–426.
Bulat, S.A. and Mironenko, N.V., Identification and Genetic Analysis of Fungi by the Polymerase Chain Reaction (PCR) Technique with Specific and Nonspecific Primers, Genetika, 1996, vol. 32, no. 2, pp. 165–183.
Naumov, G.I., Naumova, E.S., Kondratieva, V.I., Bulat, S.A., Mironenko, N.V., Mendonca-Hagler, L.C., and Hagler, A.N., Genetic and Molecular Delineation of Three Sibling Species in Hansenula polymorpha Complex, Syst. Appl. Microbiol., 1997, vol. 20, pp. 50–56.
Naumov, G.I., Tokareva, N.G., Naumova, E.S., and Bab'eva, I.P., Differentiation of the Soil Yeasts Williopsis saturnus and Williopsis suaveolens by the Polymerase Chain Reaction Technique with Nonspecific Primers, Mikrobiologiya, 2000, vol. 69, no. 2, pp. 280–285.
Naumova, E.S., Naumov, G.I., and Molina, F.I., Genetic Variation among European Strains of Saccharomyces paradoxus: Results from DNA Fingerprinting, Syst. Appl. Microbiol., 2000, vol. 23, pp. 86–92.
Yamazaki, M. and Komagata, K., An Electrophoretic Comparison of the Enzymes of Hansenula Yeasts, J. Gen. Appl. Microbiol., 1983, vol. 29, pp. 365–378.
Lewicka, K., Mallie, M., and Bastide, J.-M., Genetic Variability in the Saccharomyces Sensu Stricto Complex Revealed by Multilocus Enzyme Electrophoresis, Int. J. Syst. Bacteriol., 1995, vol. 45, pp. 538–543.
Naumov, G.I., Naumova, E.S., and Sniegowski, P.D., Differentiation of European and Far East Asian Populations of Saccharomyces paradoxus by Allozyme Analysis, Int. J. Syst. Bacteriol., 1997, vol. 47, no. 2, pp. 341–344.
Bab'eva, I.P., Reshetova, I.S., Arutyunyan, T.G., and Gorin, S.E., Soil Yeasts of the Trans-Ili Alatau, Vestn. Mosk. Univ. Ser. Biol. Pochvoved., 1975, no. 6, pp. 72–78.
Bab'eva, I.P., Reshetova, I.S., and Marchenko, A.I., Comparative Investigation of Soil Yeast Microflora in Relation to the Caucasus Altitude Belts, Biologicheskaya diagnostika pochv (Biological Diagnosis of Soils), Moscow: Nauka, 1976, pp. 27–28.
Golubev, V.I. and Blagodatskaya, V.M., Intra-and Intergeneric Ranges of Action of the Pichia punctispora (Melard 1910) Dekker 1931 Mycocin, Mikrobiologiya, 1994, vol. 63, no. 4, pp. 637–642.
Felsenstein, J., PHYLIP: Phylogenetic Inference Package, Version 3.5, Seattle: University of Washington, 1993.
Bulat, S.A., Lubeck, M., Mironenko, N.V., Jensen, D.F., and Lubeck, P.S., UP-PCR Analysis and ITS1 Ribotyping of Strains of Trichoderma and Gliocladium, Mycol. Res., 1998, vol. 102, no. 8, pp. 933–943.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Naumova, E.S., Tokareva, N.G., Bab'eva, I.P. et al. Molecular Genetic Analysis of the Yeast Komagataea (Williopsis) pratensisStrains Isolated from the Caucasian and Tien Shan Soils. Microbiology 70, 200–205 (2001). https://doi.org/10.1023/A:1010433631011
Issue Date:
DOI: https://doi.org/10.1023/A:1010433631011