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Effect of Garlic Oil on the Levels of Various Enzymes in the Serum and Tissue of Streptozotocin Diabetic Rats

Abstract

Levels of red cell, serum acid, and alkaline phosphatases, serum amylase, alanine and aspartate transferase and bilirubin were examined in streptozotocin-induced diabetic rats treated with garlic oil and compared with the corresponding levels in diabetic control rats, normal rats and normal rats on garlic oil. Values of tissue amylase and total protein were also assessed from the pancreas, liver, and kidney. Treatment of diabetic rats with garlic oil significantly decreased the red cell phosphatase (p<0.01), serum acid and alkaline phosphatase (p<0.001) when compared to diabetic control rats. Serum alanine and asparate transferases were significantly (p<0.001) decreased as well as serum amylase (p<0.002) in garlic oil treated diabetic rats as compared with diabetic control rats. When treated with garlic oil, however, diabetic and normal rats showed significant increase (p<0.05) in the amylase levels of the pancrease, liver, and kidney.

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REFERENCES

  1. 1.

    Dalvi, R. R. (1992) Alterations in hepatic phase I and phase II biotransformation enzymes by garlic oil in rats. Toxicol. Lett. 60:299-305.

    Google Scholar 

  2. 2.

    Adoga, G. I. and Ibrahim, M. B. (1990) Effects of garlic oil on some biochemical parameters in streptozotocin induced diabetic rats. Med. Sci. Res. 18:859-860.

    Google Scholar 

  3. 3.

    Horie, T. et al. (1989) Protection of liver microsomal membranes from lipid peroxidation by garlic extract. Planta Medica 55:506-508.

    Google Scholar 

  4. 4.

    Okamoto, H. (1990) The molecular basis of experimental diabetes. In: Molecular Biology of the Islets of Langerhans (H. Okamoto, ed.), Cambridge University Press, pp. 209-231.

  5. 5.

    Ohaeri, O. C. (1999) Renal function and calcium metabolism in streptozotocin—diabetic rats treated with garlic oil. Med. Sci. Res. 27:443-444.

    Google Scholar 

  6. 6.

    Reitman, S. and Frankel, S. (1957) Colorimetric method for the determination of serum transaminase. Amer. J. Clin. Pathol. 28:56-61.

    Google Scholar 

  7. 7.

    Henry, R. J. and Chiamori, N. (1959) Determination of acid and alkaline phosphatase. Clin. Chem. 5:402.

    Google Scholar 

  8. 8.

    Henry, R. J. and Chiamori, N. (1970) Saccharogenic method for amylase determination. In: Fundamentals of Clinical Chemistry (N. W. Tietz, ed.), W. B. Saunders Co., Philadelphia, pp. 414-415.

    Google Scholar 

  9. 9.

    Reinhold, J. G. (1962) Total protein, albumin and globulin. In: Standard Methods of Clinical Chemistry (D. Seligson, ed.), Academy Incorporated, New York, p. 114.

    Google Scholar 

  10. 10.

    Stroev, E. A. and Makarova, V. G. (1988) Determination of bilirubin and its fractions in blood serum. In: Laboratory Manual in Biochemistry (E. A. Stroev and V. G. Makarova, eds.), Mir Publishers, Moscow, pp. 178-179.

    Google Scholar 

  11. 11.

    Screenan, S., Pick, A. J., Levisetti, M., Baldwin, A. C., Pugh, W., and Polonsky, K. S. (1999) Increased β-cell proliferation and reduced mass before diabetes onset in the nonobese diabetic mouse. Diabetes 48:989-996.

    Google Scholar 

  12. 12.

    Eastham, R. D. (1982) In: Biochemical Values in Clinical Medicine (R. D. Eastham, ed.), John Wright and Son, Bristol, pp. 150-185, 281–283.

    Google Scholar 

  13. 13.

    Peach, H., Compston, J. E., Verdi, S., and Horton, W. L. (1982) Values of plasma, calcium, phosphorus and alkaline phosphatase measurements in the diagnosis of oeseomalacia. J. Clin. Pathol. 35:625-630.

    Google Scholar 

  14. 14.

    Sakamoto, T., Zhu, X. G., and Thompson, J. C. (1983) Hepatic inactivation of CCK-8 and CCK-33 in dogs. Gastroenterol. 84:1293.

    Google Scholar 

  15. 15.

    Unnikrishnan, M. C. and Kuttan, R. (1990) Tumour reducing and anticarcinogenic activity of selected spices. Cancer Lett. 51:75-89.

    Google Scholar 

  16. 16.

    Murray, R. K., Granner, D. K., Mayes, P. A., and Rodwell, V. W. (1993) Catabolism of proteins and of amino acid nitrogen. In: Harper's Biochemistry, 23rd edn. A Lange Medical Book. Prentice-Hall International Limited, London, pp. 293-302.

    Google Scholar 

  17. 17.

    Matthew, C. K. and Van Holde, K. E. (1993) Metabolism of nitrogenous compounds: Principles of biosynthesis, utilization, turnover, and excretion. In: Biochemistry, The Benjamin-Cummings Publishing Co. Inc., California, p. 682.

    Google Scholar 

  18. 18.

    Laakso, M. (1999) Hyperglycemia and cardiovascular disease in type 2 diabetes. Diabetes 48:937-942.

    Google Scholar 

  19. 19.

    The Diabetes Control and Complications Trail Research Group (1993) The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. New England J. Med. 329:977-986.

    Google Scholar 

  20. 20.

    Adoga, G. I. and Ohaeri, O. C. (1991) Effects of garlic oil on prothrombin, thrombin in and partial thromboplastin times in streptozotocin induced diabetic rats. Med. Sci. Res. 19:407-408.

    Google Scholar 

  21. 21.

    Bazin, R. and Lavin, M. (1979) Diet composition and insulin effect on amylase to lipase ratio in pancreas of diabetic rats. Digestion 19:386-391.

    Google Scholar 

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Ohaeri, O.C. Effect of Garlic Oil on the Levels of Various Enzymes in the Serum and Tissue of Streptozotocin Diabetic Rats. Biosci Rep 21, 19–24 (2001). https://doi.org/10.1023/A:1010425932561

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  • enzymes
  • garlic oil
  • streptozotocin diabetes