Abstract
Egg deposition of the elm leaf beetle Xanthogaleruca luteola causes the emission of volatiles from its food plant, Ulmus minor. These volatiles are exploited by the egg parasitoid, Oomyzus gallerucae, to locate its host. In contrast to other tritrophic systems, the release of volatiles is not induced by feeding but by egg deposition. Previous investigations showed that the release is systemic and can be triggered by jasmonic acid. Comparison of headspace analysis revealed similarities in the blend of volatiles emitted following egg deposition and feeding. The mixture consists of more than 40 compounds; most of the substances are terpenoids. Leaves next to those carrying eggs emit fewer compounds. When treated with jasmonic acid, leaves emit a blend that consists almost exclusively of terpenoids. Dichloromethane extracts of leaves treated with jasmonic acid were also investigated. After separation of extracts of jasmonate induced elm leaves on silica, we obtained a fraction of terpenoid hydrocarbons that was attractive to the parasitoids. This indicates that jasmonic acid stimulates the production of terpenoid hydrocarbons that convey information of egg deposition to the parasitoid.
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REFERENCES
Baldwin, I. T., and Preston, C. A. 1999. The eco-physiological complexity of plant responses to insect herbivores. Planta 208:137-145.
Baldwin, I. T., Schmelz, E. A., and Ohnmeiss, T. E. 1994. Wound-induced changes in root and shoot jasmonic acid pools correlate with induced nicotine synthesis in Nicotiana sylvestris Spegazzini and Comes. J. Chem. Ecol. 20:2139-2157.
Boland, W., Ney, P., Jänicke, L., and Gassmann, G. 1984. A “Closed-loop-stripping” technique as a versatile tool for metabolic studies of volatiles, pp. 371-380, in P. Schreier (ed.). Analysis of Volatiles: Method, Applications, de Gruyter. Berlin.
Boland, W., Hopke, J., Donath, J., Nüske, J., and Bublitz, F. 1995. Jasmonic acid and coronatine induce odor production in plants. Angew. Chem. Int. Ed. Engl. 34:1600-1602.
Bortz, J. 1993. Statistik für Sozialwissenschaftler, 4th ed. Springer. Berlin.
Bülow, N., and König, W. A. 2000. The role of germacrene D as a precursor in sesquiterpene biosynthesis: Investigations of acid catalyzed, photochemically and thermally induced rearrangements. Phytochemistry 55:141-168.
Creelman, R. A., and Mullet, J. E. 1997. Biosynthesis and action of jasmonates in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:355-381.
Dicke, M. 1994. Local and systemic production of volatile herbivore-induced terpenoids: Their role in plant-carnivore mutualism. J. Plant Physiol. 143:465-472.
Dicke, M., and Vet, L. 1999. Plant-carnivore interactions: Evolutionary and ecological consequences for plant, herbivore and carnivore, pp. 483-520, in H. Olff, V. K. Brown, and R. H. Drent (eds.). Herbivores: Between Plants and Predators. Blackwell Science, Oxford.
Dicke, M., Van beek, T. A., Posthumus, M. A., Ben dom, N., Van bokhoven, H., and De groot, A. 1990. Isolation and identification of volatile kairomone that effects predator-prey interactions. Involvement in host plant in its prdocution. J. Chem. Ecol. 16:381-396.
Dicke, M., Van baarlen, P., Wessels, R., and Dijkman, H. 1992. Herbivory induces systemic production of plant volatiles that attract predators of the herbivore: Extraction of endogenous elicitor. J. Chem. Ecol. 19:581-599.
Dicke, M., Gols, R., Ludeking, D., and Posthumus, M. A. 1999. Jasmonic acid and herbivory differentially induce carnivore-attracting plant volatiles in lima bean plants. J. Chem. Ecol. 25:1907-1922.
Du, Y., Poppy, G. M., Powell, W., Picket, J. A., Wadhams, L. J., and Woodcock, C. M. 1998. Identification of semiochemicals released during aphid feeding that attract parasitoid Aphidus ervi. J. Chem. Ecol. 24:1355-1368.
Farmer, E. E., and Ryan, C. A. 1992. Octadecanoid precursors of jasmonic acid activate the synthesis of wound-inducible proteinase inhibitors. Plant Cell 4:129-134.
Garnero, J., and Tabacchi, R. 1987. Examples of artefact formation by chromatographic techniques, pp. 359-367, in P. Sandra, and C. Bicchi (eds.). Capillary Gas Chromatography in Essential Oil Analysis. Dr. Alfred Huethig Verlag, Heidelberg.
Gols, R., Posthumus, M. A., and Dicke, M. 1999. Jasmonic acid induces the production of gerbera volatiles that attract the biological control agent Phytoseiulus persimilis. Entomol. Exp. Appl. 93:77-86.
Joulain, D., and König, W. A. 1998. The Atlas of Spectral Data of Sesquiterpene Hydrocarbons. EB-Verlag, Hamburg.
Kaiser, R. 1991. Trapping, investigation and reconstitution of flower scents, pp. 213-250, in P. M. Müller and D. Lamprarsky (eds.). Perfumes, Art, Science and Technology. Elsevier Applied Science, London.
Koch, T., Krumm, T., Jung, V. Engelberth, J., and Boland W. 1999. Differential induction of plant volatile biosynthesis in the lima bean by early and late intermediates of the octadecanoid-signaling pathway. Plant Physiol. 121:153-162.
Mattiacci, L., Dicke, M., and Posthumus, M. A. 1995. ?-Glucosidase: An elicitor of herbivore induced plant odor that attracts host-searching parasitic wasps. Proc. Natl. Acad. Sci. U.S.A. 92:2036-2040.
Meiners, T., and Hilker, M. 1997. Host location in Oomyzus gallerucae (Hymenoptera: Eulophidae), an egg parasitoid of the elm leaf beetle Xanthogaleruca luteola (Coleoptera: Chrysomelidae). Oecologia 112:87-93.
Meiners, T., and Hilker, M. 2000. Induction of plant synomones by oviposition of a phytophagous insect. J. Chem. Ecol. 26:221-232.
Miksch, M., and Boland, W. 1996. Airborne methyl jasmonate stimulates the biosynthesis of furanocoumarins in the leaves of celery plants (Apium graveolescens). Experientia 52:739-743.
Paré, P. W., and Tumlinson, J. H. 1997. De novo biosynthesis of volatiles induced by insect herbivory in cotton plants. Plant Physiol. 114:1161-1167.
Paré, P. W., and Tumlinson, J. H. 1998. Cotton volatiles synthesized and released distal to the site of insect damage. Phytochemistry 47:521-526.
Paré, P. W., and Tumlinson, J. H. 1999. Plant volatiles as a defense against insect herbivores. Plant Physiol. 121:325-331.
Sembdner, G., and Parthier, B. 1993. The biochemistry and the physiological and molecular actions of jasmonates. Annu. Rev. Plant Physiol. Plant Mol. Biol. 44:569-589.
Takabayashi, J., Dicke, M., and Posthumus, M. A. 1994. Volatile herbivore-induced terpenoids in plant-mite interactions: Variation caused by biotic and abiotic factors. J. Chem. Ecol. 20:1329-1354.
Teisseire, P. J. 1994. Chemistry of Fragrant Substances. VCH publishers, New York, pp. 200-218.
Turlings, T. C. J., Tumlinson, J. H., and Lewis, W. J. 1990. Exploitation of herbivore-induced plant odors by host seeking parasitic wasps. Science 250:1251-1253.
Turlings, T. C. J., Mccall, P. J., Alborn, H., and Tumlinson, J. H. 1993. An elicitor in caterpillar oral secretions that induces corn seedlings to emit chemical signals attractive to parasitic wasps. J. Chem. Ecol. 19:412-425.
Turlings, T. C. J., Loughrin, J. H., Mccall, P. J., Röse, U. S. R., Lewis, W. J., and Tumlinson, J. H. 1995. How caterpillar-damaged plants protect themselves by attracting parasitic wasps. Proc. Natl. Acad. Sci. U.S.A. 92:4169-4174.
Turlings, T. C. J., Lengwiler, U. B., Bernasconi, M. L., and Wechsler, D. 1998. Timing of induced volatile emissions in maize seedlings. Planta 207:146-152.
Van dort, H. M., Jägers, P. P., Ter heide, R., and Van der weerdt, J. A. 1993. Narcissus trevithian and Narcissus geranium: Analysis and synthesis of compounds. J. Agric. Food Chem. 41:2063-2075.
Vet, L. E. M., Van lenteren, J. C., Heymanns, M., and Meelis, E. 1983. An airflow olfactometer for measuring olfactory responses of hymenopterous parasitoids and other small insects. Physiol. Entomol. 8:97-106.
Vinson, S. B. 1985. The behavior of parasitoids, pp. 417-469, in G. A. Kerkut and L. I. Gilbert (eds.). Comprehensive Insect Physiology, Biochemistry and Pharmacology. Pergamon Press, New York.
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Wegener, R., Schulz, S., Meiners, T. et al. Analysis of Volatiles Induced by Oviposition of Elm Leaf Beetle Xanthogaleruca luteola on Ulmus minor. J Chem Ecol 27, 499–515 (2001). https://doi.org/10.1023/A:1010397107740
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DOI: https://doi.org/10.1023/A:1010397107740