Skip to main content
Log in

Stress effect of ethanol on fermentation kinetics by stationary-phase cells of Saccharomyces cerevisiae

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

When 4% (v/v) ethanol was added progressively to two strains exhibiting different fermentative abilities, K1 (a commercial wine strain) and V5 (a strain derived of a wine yeast), the fermentation rate correlated directly to the ethanol concentration for both strains. In contrast, the effect of sudden addition of 2%, 4% or 6% (v/v) ethanol was different depending on the strain. While the same effect was observed for K1 whatever the way of ethanol addition, V5 required an adaptation period after the shock addition of ethanol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Alexandre H, Rousseaux I, Charpentier C (1993) Ethanol adaptation mechanisms in Saccharomyces cerevisiae. Biotechnol. Appl. Biochem. 20: 173-183.

    Google Scholar 

  • Alexandre H, Rousseaux I, Charpentier C (1994) Relationship between ethanol tolerance, lipid composition and plasma membrane fluidity in Saccharomyces cerevisiae and Kloeckera apiculata. FEMS Microbiol. Lett. 124: 17-22.

    Google Scholar 

  • Bely M, Sablayrolles JM, Barre P (1990) Automatic detection of assimilable nitrogen deficiencies during alcoholic fermentation in oenological conditions. J. Ferment. Bioeng. 70: 246-252.

    Google Scholar 

  • Brown SW, Oliver SG, Harrison DEF, Righelato RC (1981) Ethanol inhibition of yeast growth and fermentation: differences in the magnitude and complexity of the effect. Eur. J. Appl. Microbiol. Biotechnol. 11: 151-155.

    Google Scholar 

  • Casey GP, Magnus CA, Ingledew WM (1984) High-gravity brewing: effects of nutrition on yeast composition, fermentative ability and alcohol production. Appl. Environ. Microbiol. 48: 639-646.

    Google Scholar 

  • D'Amore T, Panchal CJ, Russell I, Stewart GG (1990) A study of ethanol tolerance in yeast. Crit. Rev. Biotechnol. 9: 287-304.

    Google Scholar 

  • El Haloui N, Picque D, Corrieu G (1988) Alcoholic fermentation in winemaking: on line measurement of density and carbon dioxyde evolution. J. Food. Eng. 8: 17-30.

    Google Scholar 

  • Holzberg LO, Finn RF, Steinkraus KH (1967) A kinetic study of the alcoholic fermentation of grape juice. Biotechnol. Bioeng. 9: 413-423.

    Google Scholar 

  • Ingram LO, Buttke TM (1984) Effects of alcohols on microorganisms. Adv. Microb. Physiol. 25: 253-300.

    Google Scholar 

  • Jones RP, Greenfield PF (1985) Replicative inactivation and metabolic inhibition in yeast ethanol fermentations. Biotechnol. Lett. 7: 223-228.

    Google Scholar 

  • Kalmokoff ML, Ingledew WM (1985) Evaluation of ethanol tolerance in selected Saccharomyces strains. J. Am. Soc. Brew. Chem. 43: 189-196.

    Google Scholar 

  • King LM, Schisler DO, Ruocco JJ (1981) Epifluorescent method for detection of nonviable yeast. J. Am. Soc. Brew. Chem. 39: 52-54.

    Google Scholar 

  • Leão C, Van Uden N (1982) Effects of ethanol and other alkanols on the glucose transport system of Saccharomyces cerevisiae. Biotechnol. Bioeng. 24: 2601-2604.

    Google Scholar 

  • Mauricio JC, Salmon JM (1992) Apparent loss of sugar transport activity in Saccharomyces cerevisiae. Biotechnol. Lett. 14: 577-582.

    Google Scholar 

  • Monteiro GGA, Sa-Correia I (1998) In vivo activation of yeast plasma membrane H+-ATPase by ethanol: effect on the kinetic parameters and involvement of the carboxyl-terminus regulatory domain. Biochim. Biophys. Acta 1370: 310-316.

    Google Scholar 

  • Mota, Strehaiano P, Goma G (1984) Studies on conjugate effects of substrate (glucose) and product (ethanol) on cell growth kinetics during fermentation of different yeast strains. J. Inst. Brew. 90: 359-362.

    Google Scholar 

  • Piper PW, Talreja K, Panaretou B, Moradas-Ferreira P, Byrne K, Praekelt UM, Meacock P, Récnacq M, Boucherie H (1994) Induction of major heat-shock proteins of Saccharomyces cerevisiae, including plasma membrane Hsp30, by ethanol levels above a critical threshold. Microbiology 140: 3031-3038.

    Google Scholar 

  • Sablayrolles JM, Barre P, Grenier P (1987) Design of laboratory automatic system for studying alcoholic fermentations in anisothermal enological conditions. Biotechnol. Tech. 1: 181-184.

    Google Scholar 

  • Salmon JM (1989) Effect of sugar transport inactivation in Saccharomyces cerevisiae on sluggish and stuck enological fermentations. Appl. Environ. Microbiol. 55: 953-958.

    Google Scholar 

  • Salmon JM, Vincent O, Mauricio JC, Bely M, Barre P (1993) Sugar transport inhibition and apparent loss of activity in Saccharomyces cerevisiae as a major limiting factor of enological fermentations. Am. J. Enol. Vitic 44: 56-64.

    Google Scholar 

  • Thomas DS, Hossack JA, Rose AH (1978) Plasma-membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae. Arch. Microbiol. 117: 239-245.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Virginie Ansanay-Galeote.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ansanay-Galeote, V., Blondin, B., Dequin, S. et al. Stress effect of ethanol on fermentation kinetics by stationary-phase cells of Saccharomyces cerevisiae. Biotechnology Letters 23, 677–681 (2001). https://doi.org/10.1023/A:1010396232420

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1010396232420

Navigation