Abstract
Bacterial contamination of drinking water wells is a commonproblem in many rural areas. Some of this contamination may berelated to manure spreading or housing of livestock; another source is on-site septic systems. Current indicator organisms are able to detect the presence of fecal contamination, but where there may be more than one potential source of fecal material, the current indicators are unable to ascertain the origin. This laboratory investigation was undertaken to determine the longevity and reliability of a selected suite of indicator organisms. Total coliform, fecal coliform, fecal streptococci and Clostridium perfringens were monitoredin a simulated contaminated groundwater environment for 6 months. All four indicator organisms were present at the end of6 months. The number of fecal streptococi bacteria decreased most noticeably, allowing assessment of relative age of contamination. C. perfringens was found to be a reliableindicator of contamination from animal manure. Fecal material from 28 different animals and three septic systems were assessed for the presence of the indicator organisms. Totalcoliform, fecal coliform and fecal streptococci were present in the fecal material of all animals tested including reptiles.C. perfringens was detected in feces from all but two of the animals assessed. Using an assemblage of indicator organisms provides more information regarding source and timingof contamination than just testing for total coliform and fecalcoliform bacteria.
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References
American Public Health Association (APHA), American Water Works Association Water Pollution Control Federation: 1989, Standard Methods for the Examination of Water and Wastewater, 17th edn., American Public Health Association, Washington.
Bitton, G.: 1994, Wastewater Microbiology, Wiley-Liss, Toronto.
Bitton, G. S., Farrah, R., Ruskin, R. H., Butner, J. and Chou, Y. J.: 1983, Ground Water 21, 405.
Conboy, M. J. and Goss, M. J.: 1998, Water Qual. Res. J of Can. 34, 281.
Crane, S. R. and Moore, J.: 1984, Water, Air, and Soil Pollut. 22, 67.
Crane, S. R. and Moore, J.: 1986, Water, Air, and Soil Pollut. 27, 411.
Doran, J. W., Schepers, J. S. and Swanson, N. P.: 1981, J. of Soil and Water Cons. 36(3), 166.
Doran, J. W. and Linn, D. M.: 1979, Appl. Environ. Micro. 37(5), 985.
Geldreich, E. E.: 1976, C. R. C. Crit. Reviews in Environ. Cont. 5, 349.
Gerba, C. P. and Keswick, B. H.: 1981, Quality of Groundwater, in W. van Duijvenbooden, P. Glasenbergen and H. van Lelyveld (eds.), Elsevier, Amsterdam, p. 511.
Goss, M. J., Barry, D. A. J. and Rudolph, D. L.: 1998, J. Contam. Hydrol. 32, 267.
Hart, Stephen: 1996, Environ. Sci. Tech. 30, 289.
Hazen, T. C.: 1988, Tox. Assess. 3, 461.
Huysman, F., Van Renterghem, B. and Verstraete, W.: 1993, Water, Air, and Soil Pollut. 69, 243.
Lynch, J. M. and Poole, N. J.: 1979, Microbial Ecology: A Conceptual Approach, Blackwell Scientific Publications, London.
Martin, G. N. and Noonan, M. J.: 1977, Water and Soil Technical Publication, Water and Soil Division, Ministry of Work and Development, New Zealand, p. 25.
Raina, P., Pollari, F. Teare, G. Goss, M. J., Barry, D. A. J. and Wilson, J. B.: 1998, Can. J. Public Health 90, 172.
Reddy, K. R., Khaleel, R. and OverCash, M. R.: 1981, J. Environ. Qual. 10, 255.
Rudolfs, W., Franks, L. L. and Ragotzkie, R. A.: 1950, Sewage Indust. Wastes. 22, 1261.
Sinton, L. W.: 1980, J. Hydrol. (N. Z.) 19, 119.
Thelin, R. and Gifford, G. F.: 1983, J. Environ. Qual. 12, 57.
Verstraete, W. and Voets, J. P.: 1976, Water Research 10, 129.
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Conboy, M.J., Goss, M.J. Identification of an Assemblage of Indicator Organisms to Assess Timing and Source of Bacterial Contamination in Groundwater. Water, Air, & Soil Pollution 129, 101–118 (2001). https://doi.org/10.1023/A:1010393927600
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DOI: https://doi.org/10.1023/A:1010393927600