Biotechnology Letters

, Volume 23, Issue 2, pp 95–101 | Cite as

Comparative characterisation of twoRhodococcusspecies as potential biocatalysts for ammonium acrylate production

  • N.A. Webster
  • D.K. Ramsden
  • J. Hughes


Two Rhodococcal isolates, one possessing a nitrile hydratase and an amidase enzyme, the other an aliphatic nitrilase enzyme have been isolated. The kinetic constants for the enzymes in each isolate have been determined. This data coupled with stability tests indicate that Rhodococcus ruber NCIMB 40757, the nitrilase containing organism, should be an excellent biocatalyst for the commercial production of ammonium acrylate. This is confirmed by a fed-batch bioconversion to produce 5.7 M ammonium acrylate.

acrylate amidase bioreactor nitrilase nitrile hydratase Rhodococcus 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • N.A. Webster
    • 1
  • D.K. Ramsden
    • 1
  • J. Hughes
  1. 1.Biotechnology Centre, Department of Chemical and Biological SciencesUniversity of Huddersfield, QueensgateHuddersfield, West YorkshireUK

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