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Hydrogen Evolution from L-Lactate and L-Malate with the Combination of Lactate Dehydrogenase or Malate Dehydrogenase and Hydrogenase from A. Eutrophus H16

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Abstract

Hydrogen evolution system from L-lactate and L-malate consisting of lactate dehydrogenase or malate dehydrogenase and hydrogenase from cell free extracts of Alcaligenes eutrophus H16 was established. When the solution containing L-lactate, lactate dehydrogenase, NAD and hydrogenase was incubated at 30°C hydrogen evolution was observed. Similarly, the hydrogen evolution was also observed from the L-malate incubated at 30°C.

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Authors and Affiliations

  1. Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan

    Niranjan Kumar Shrestha, Toshiaki Kamachi & Ichiro Okura

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  1. Niranjan Kumar Shrestha
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  2. Toshiaki Kamachi
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  3. Ichiro Okura
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Shrestha, N.K., Kamachi, T. & Okura, I. Hydrogen Evolution from L-Lactate and L-Malate with the Combination of Lactate Dehydrogenase or Malate Dehydrogenase and Hydrogenase from A. Eutrophus H16. Reaction Kinetics and Catalysis Letters 70, 287–292 (2000). https://doi.org/10.1023/A:1010388816718

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  • DOI: https://doi.org/10.1023/A:1010388816718

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