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High-density culture of recombinant Chinese hamster ovary cells producing prothrombin in protein-free medium

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Abstract

A recombinant CHO cell line, CHO2DS, was immobilized on porous microcarrier Cytopore 1 and cultivated in 1 l modified Super-spinner and 2 l stirred tank bioreactor with the perfusion of a low-cost chemically defined protein-free medium DF6S. CHO2DS cells could enter into the inner space and grew both in the inner space and on the surface of Cytopore 1 in DF6S and produced prothrombin at 22 mg l−1 after 10 days. From a seeding density of 5.7 × 105 cells ml−1, the highest viable cell density of CHO2DS was 1.12 × 107 cells ml−1.

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Authors and Affiliations

  1. Department of Cell Engineering, Institute of Biotechnology, 20 Dongda Street, Fengtai, Beijing, 100071

    Zhaolie Chen

  2. Institute of Cell Culture Technology, University of Bielefeld, P.O. Box 100131, 33501, Bielefeld, Germany

    Dirk Lütkemeyer, Kai Iding & Jürgen Lehmann

Authors
  1. Zhaolie Chen
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  2. Dirk Lütkemeyer
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  3. Kai Iding
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  4. Jürgen Lehmann
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Chen, Z., Lütkemeyer, D., Iding, K. et al. High-density culture of recombinant Chinese hamster ovary cells producing prothrombin in protein-free medium. Biotechnology Letters 23, 767–770 (2001). https://doi.org/10.1023/A:1010373527375

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  • DOI: https://doi.org/10.1023/A:1010373527375

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