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Optimisation of medium composition for clavulanic acid production by Streptomyces clavuligerus

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Abstract

Among four different commercially available nitrogen sources containing soybean derivatives, a protein extract of soybean gave the highest yield for clavulanic acid production by Streptomyces clavuligerus. A statistical method based on factorial design of experiments was applied to optimise the medium. An empirical model was obtained by applying response surface statistical analysis. The analysis of variance showed that concentrations of protein extract of soybean and glycerol and the interaction between these two variables were significant at 95% level of confidence. The maximum clavulanic acid concentration obtained in 72 h was 1.2 g l−1.

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of S. Carlos, CEP 13565-905, S. Carlos, SP, Brazil

    Ester R. Gouveia, Alvaro Baptista-Neto, Alberto C. Badino Jr & Carlos O. Hokka

Authors
  1. Ester R. Gouveia
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  2. Alvaro Baptista-Neto
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  3. Alberto C. Badino Jr
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  4. Carlos O. Hokka
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Corresponding author

Correspondence to Alberto C. Badino Jr.

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Gouveia, E.R., Baptista-Neto, A., Badino Jr, A.C. et al. Optimisation of medium composition for clavulanic acid production by Streptomyces clavuligerus . Biotechnology Letters 23, 157–161 (2001). https://doi.org/10.1023/A:1010356231673

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  • DOI: https://doi.org/10.1023/A:1010356231673

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