Abstract
The nitrifying bacteria in activated sludge and biofilms consisting of the bacteria immobilized on polypropylene packing were subjected to an electric current via two electrodes. In activated sludge, the metabolism of nitrifying bacteria was inhibited when the applied current was over 2.5 A m−2, whilst in biofilms, inhibition began when the current reached 5 A m−2. At 15 A m−2, the nitrification rate of NH4 +-N in a biofilm with a bacterial density of 1.62 g total solids, dry wt m−2 decreased to about 80% of its initial value. Ninety-two % of the initial biomass on the packing was retained after 36 h.
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References
APHA, AWWA, WPCF (1989) Standard Methods for the Examination of Water and Wastewater, 17th edn. New York: American Public Health Association, American Water Works Association and Water Pollution Control Federation.
Findlay RH, King GM, Watling L (1989) Efficacy of phospholipid analysis in determining microbial biomass in sediments. Appl. Environ. Microbiol. 55: 2888-2893.
Grady LCP, Daigger GT, Lim HC (1999) Biological Wastewater Treatment. New York: Marcel Dekker, Inc.
Jackman SA, Maini G, Sharman AK, Knowles CJ (1999) The effects of direct electric current on the viability and metabolism of acidophilic bacteria. Enzyme Microbiol. Technol. 24: 316-324.
Mizuno A, Hori Y (1988) Destruction of living cells by pulsed high-voltage application. IEEE Trans. Ind. Appl. 24: 387-393.
Sakakibara Y, Kuroda M (1993) Electric prompting and control of denitrification. Biotechnol. Bioeng. 42: 535-537.
Tokuda H, Nakanishi K (1995) Application of direct current to protect bioreactor against contamination. Biosci. Biotech. Biochem. 59: 753-755.
Watanabe Y, Masuda S, Ishiguro M (1992) Simultaneous nitrification and denitrification in micro-aerobic biofilm. Water Sci. Technol. 26: 511-522.
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Li, XG., Cao, HB., Wu, JC. et al. Inhibition of the metabolism of nitrifying bacteria by direct electric current. Biotechnology Letters 23, 705–709 (2001). https://doi.org/10.1023/A:1010346501857
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DOI: https://doi.org/10.1023/A:1010346501857