Abstract
Studies of Sprague-Dawley rats using in vivo intracerebral dialysis and high-performance liquid chromatography with electrochemical detection were used to investigate glycine release into the intercellular space of the nucleus accumbens during food consumption. The results showed that food consumption led to decreases in glycine levels in the intercellular space of the nucleus accumbens. Administration of the sodium channel blocker tetrodotoxin (1 μM), but not the glutamate reuptake blocker D,L-threo-β-hydroxyaspartate (1 mM), prevented the food-related behavior-induced decrease in glycine levels in the nucleus accumbens. Eating of food after administration of the dopamine D2 receptor blocker raclopride (10 μM) into the nucleus accumbens was accompanied by an increase in the glycine level in the intercellular space of this structure. These data provide evidence for the neural regulation of glycine release in the nucleus accumbens during food-related behavior, mediated via dopamine D2 receptors.
Similar content being viewed by others
REFERENCES
N. B. Saul'skaya and Ch. A. Marsden, “Involvement of the glutamatergic input of the nucleus accumbens in controlling the synaptic release of dopamine during associative learning,” Ros. Fiziol. Zh. im. I. M. Sechenova, 80, No. 12, 45–54 (1994).
K. B. Shapovalova, A. I. Gorbachevskaya, and N. B. Saul'skaya, “Structural and neurochemical mechanisms of the involvement of the nucleus accumbens in the interaction of the limbic and motor systems and the regulation of movement behavior,” Zh. Vyssh. Nerv. Deyat., 42, No. 2, 226–276 (1992).
G. M. Alexander, J. R. Grothusen, S. W. Gordon, and R. J. Schwartzman, “Intracerebral microdialysis study of glutamate reuptake in awake, behaving rats,” Brain Res., 766, No. 1/2, 1–10 (1997).
G. Bustos, M. I. Forray, K. Gysling, C. W. Bradberry, and R. H. Roth, “Regulation of excitatory amino acid release by N-methyl-D-aspartate receptors in rat striatum. In vivo microdialysis studies,” Brain Res., 585, No. 1, 101–115 (1992).
W. Danysz and C. C. Parsons, “Glycine and N-methyl-D-aspartate receptors. Physiological significance and possible therapeutic application,” Pharmacol. Rev., 50, No. 4, 597–664 (1998).
K. N. Gracy and V. M. Pickel, “Ultrastructural immunocytochemical localizations of N-methyl-D-aspartate receptors and tyrosine hydroxylase in the shell of the rat nucleus accumbens,” Brain Res., 739, No. 1/2, 169–184 (1996).
M. O. Krebs, M. L. Kemel, C. Gauchy, M. Desban, and J. Glovinski, “Glycine potentiates the NMDA-induced release of dopamine through a strychnine insensitive site in the rat striatum,” Eur. J. Pharmacol., 166, 567–570 (1989).
E. F. La Gamma, E. Strecker, N. J. Lenn, J. D. DeCristofaro, and G. Weisinger, “Dopamine regulation of transfected preproenkephaline promoter in primary rat astrocytes in vitro and in vivo,” Exptl. Neurology, 130, No. 2, 304–310 (1994).
C. S. Maldonado-Irizarry, C. J. Swanson, and A. E. Kelley, “Glutamate receptors in the nucleus accumbens shell control feeding behaviour via the lateral hypothalamus,” J. Neurosci., 15, No. 10, 6779–9788 (1995).
P. Rada, S. Tucci, E. Murzi, and L. Hermandez, “Extracellular glutamate increases in the lateral hypothalamus and decreases in the nucleus accumbens during feeding,” Brain Res., 768, No. 1/2, 338–340 (1997).
C. Rampon, P. H. Luppi, P. Fort, C. Peyron, and M. Jouvet, “Distribution of glycine-immunoreactive cell bodies and fibers in the rat brain,” Neurosci., 75, No. 3, 737–755 (1996).
H. L. Rowley, K. F. Martin, and C. A. Marsden, “Determination of in vivo amino acid neurotransmitters by high performance liquid chromatography with ophthaldehyde-sulphate derivatisation,” J. Neurosci. Meth., 57, No. 1, 93–99 (1995).
F. Zafra, C. Aragon, L. Olivares, N. C. Danbolt, C. Gimenez, and J. Storm-Mathisen, “Glycine transporters are differentially expressed among CNS cells,” J. Neurosci., 15, No. 5, 3952–3969 (1995).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Saul'skaya, N.B., Mikhailova, M.O. & Gorbachevskaya, A.I. Dopamine-Dependent Inhibition of Glycine Release in the Nucleus Accumbens of the Rat Brain during Food Consumption. Neurosci Behav Physiol 31, 317–321 (2001). https://doi.org/10.1023/A:1010342803617
Issue Date:
DOI: https://doi.org/10.1023/A:1010342803617