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Production of a novel bioflocculant by fed-batch culture of Citrobacter sp.

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Abstract

Production of a novel bioflocculant by a fed-batch culture of Citrobacter sp. TKF04 was investigated using acetic acid as a sole carbon source. Synthesis of the bioflocculant was favored by dissolved O2 tension at 20% of air saturation and C/N ratio (mol acetic acid/mol ammonium) of 10:1 in the feed solution. Under optimal conditions, 4.6 g crude bioflocculant per liter broth was produced, whose flocculating activity was 22 300 units. This activity was 9 times higher than that of the control (only acetic acid was supplied).

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References

  • Dearfield KL, Albernathy CO, Ottley MS, Brantner JH, Hayes PF (1988) Acrylamide: its metabolism, developmental and reproductive effects, genotoxicity, and carcinogenicity. Mut. Res. 195: 45-77.

    Google Scholar 

  • Fujita M, Ike M, Tachibana S, Kitada G, Kim SM, Inoue Z (2000) Characterization of a bioflocculant produced by Citrobacter sp. TKF04 from acetic acid and propionic acids. J. Biosci. Bioeng. 89: 40-46.

    Google Scholar 

  • Jang JH, Rogers PL (1995) High productivity biopolymer production by Alcaligenes sp. SH-69 using two stage continuous culture. Aust. J. Biotechnol. 5: 104-106.

    Google Scholar 

  • Jang JH, Rogers PL (1996) Effect of levulinic acid on cell growth and poly-β-hydroxyalkanoate production by Alcaligenes sp. SH-69. Biotechnol. Lett. 18: 219-224.

    Google Scholar 

  • Kallos MS, Behie LA (1999) Inoculation and growth conditions for high-cell-density expansion of mammalian neural stem cells in suspension bioreactors. Biotechnol. Bioeng. 63: 473-483

    Google Scholar 

  • Kowall NW, Pendlebury WW, Kessler JB, Perl DP, Beal MF (1989) Aluminum-induced neurofibrillary degeneration affects a subset of neurons in rabbit cerebral cortex, basal forebrain and upper brainstem. Neuroscience 29: 329-337.

    Google Scholar 

  • Kurane R, Takeda K, Suzuki T (1986) Screening for and characteristics of microbial flocculants. Agric. Biol. Chem. 50: 2301-2307.

    Google Scholar 

  • Pullen RGL, Candy JM, Morris CM, Taylor G, Keith AB, Edwardson JA (1990) Gallium-67 as a potential marker for aluminum transport in rat brain: implications for Alzheimer's disease. J. Neurochem. 55: 251-259.

    Google Scholar 

  • Ramsay BA, Lomaliza K, Chavarie C, Dubé B, Bataille P, Ramsay JA (1990) Production of poly-β-hydroxybutyric-co-β-hydroxyvaleric) acids. Appl. Environ. Microbiol. 56: 2093-2098.

    Google Scholar 

  • Rhee YH, Jang JH, Rogers PL (1993) Production of copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate by fed-batch culture of Alcaligenes sp. SH-69. Biotechnol. Lett. 15: 377-382.

    Google Scholar 

  • Vanhorick M, Moens W (1983) Carcinogen-mediated induction of SV 40 DNA amplification is enhanced by acrylamide in Chinese hamster CO60 cells. Carcinogenesis 4: 1459-1463.

    Google Scholar 

  • Yokoi H, Shiraki M, Hirose J, Hayashi S, Takasaki Y (1996) Flocculation properties of xanthan produced by Xanthomonas campestris. Biotechnol. Tech. 10: 789-792.

    Google Scholar 

  • Zhong JJ, Yoshida T (1995) High-density cultivation of Perilla frutescens cell suspensions for anthocyanin production: effects of sucrose concentration and inoculum size. Enzyme Microb. Technol. 17: 1073-1079.

    Google Scholar 

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Correspondence to Jin-Ho Jang.

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Jang, JH., Ike, M., Kim, S.M. et al. Production of a novel bioflocculant by fed-batch culture of Citrobacter sp.. Biotechnology Letters 23, 593–597 (2001). https://doi.org/10.1023/A:1010312607171

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  • DOI: https://doi.org/10.1023/A:1010312607171

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