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High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector

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Abstract

Six regions of the VP4 protein of bovine rotavirus strain UKtc were expressed using hepatitis B core antigen (HBcAg) as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20–37% of total cellular protein. Soluble fusion proteins in a particulate form were partially purified with yields ranging from 0.5–6.4 mg l−1 of culture.

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Authors and Affiliations

  1. School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia

    Nazlina Ibrahim

  2. Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

    Malcolm A. McCrae

Authors
  1. Nazlina Ibrahim
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  2. Malcolm A. McCrae
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Ibrahim, N., McCrae, M.A. High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector. Biotechnology Letters 23, 643–646 (2001). https://doi.org/10.1023/A:1010310232033

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  • DOI: https://doi.org/10.1023/A:1010310232033

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