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Chemo-enzymatic synthesis of 3-(2-naphthyl)- L-alanine by an aminotransferase from the extreme thermophile, Thermococcus profundus

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Abstract

Hyper-thermostable aminotransferase from Thermococcus profundus (MsAT) was used to synthesize 3-(2-naphthyl)-l-alanine (Nal) by transamination between its corresponding α-keto acid, 3-(2-naphthyl)pyruvate (NPA) and l-glutamate (Glu) at 70 °C. Equilibrium of this reaction was shifted toward Nal production due to its low solubility, giving rise to Nal precipitate. Optically pure Nal (>99% ee) was synthesized with 93% (mol mol−1) yield from 180 mM NPA and 360 mM Glu.

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Authors and Affiliations

  1. Tokyo Research Center, Tosoh Corporation, 2743-1 Hayakawa, Ayase-shi, Kanagawa, 252-1123, Japan

    Satoshi Hanzawa, Seigo Oe, Kenji Tokuhisa, Kazuhisa Kawano & Hitoshi Kakidani

  2. Department of Biological Mechanisms and Function, Graduate School of Bioagricultural Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya-shi, Aichi, 464-8601, Japan

    Tetsuo Kobayashi

  3. Laboratory of Microbiology, The Institute of Physical and Chemical Research (RIKEN), 2-1, Hirosawa, Wako-shi, Saitama, 351-0198, Japan

    Toshiaki Kudo

Authors
  1. Satoshi Hanzawa
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  2. Seigo Oe
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  3. Kenji Tokuhisa
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  4. Kazuhisa Kawano
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  5. Tetsuo Kobayashi
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  6. Toshiaki Kudo
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  7. Hitoshi Kakidani
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Hanzawa, S., Oe, S., Tokuhisa, K. et al. Chemo-enzymatic synthesis of 3-(2-naphthyl)- L-alanine by an aminotransferase from the extreme thermophile, Thermococcus profundus. Biotechnology Letters 23, 589–591 (2001). https://doi.org/10.1023/A:1010308506263

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  • DOI: https://doi.org/10.1023/A:1010308506263

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