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Isolation and Study of Some Properties of Laccase from the Basidiomycetes Cerrena maxima

Biochemistry (Moscow) volume 66, pages 618–622 (2001)Cite this article

Abstract

A new strain producing extracellular laccase (Cerrena maxima 0275) was found by screening of isolates of Basidiomycetes, and the dynamics of laccase biosynthesis by this strain was studied. The enzyme was purified to homogeneity. The molecular weight of the enzyme is 57 kD, and its pI is 3.5. The activity is constant at pH values in the range 3.0-5.0. The temperature optimum for activity is 50°C. The thermal stability of the laccase was studied. The catalytic and Michaelis constants for catechol, hydroquinone, sinapinic acid, and K4 Fe(CN)6 were determined. The standard redox potential of type 1 copper in the enzyme is 750 ± 5 mV. Thus, the investigated laccase is a high redox potential laccase.

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REFERENCES

  1. Messerschmidt, A. (1997) in Multicopper Oxidases (Messerschmidt, A., ed.) World Scientific, Singapore-New Jersey-London-Hong Kong, pp. 23–80.

    Google Scholar 

  2. Peter, M. G., and Wollenberger, U. (1997) in Frontiers in Biosensors. Fundamental Aspects (Scheller, F. W., Shubert, F., and Fedrowitz, J., eds.) Birkhauser Verlag, Basel, pp. 63–82.

    Google Scholar 

  3. Solomon, E. I., Sundaram, U. M., and Machonkin, T. E. (1996) Chem. Rev., 96, 2563–2605.

    Google Scholar 

  4. Reinhammar, B. (1997) in Multicopper Oxidases (Messerschmidt, A., ed.) World Scientific, Singapore-New Jersey-London-Hong Kong, pp. 167–200.

    Google Scholar 

  5. Farver, O., and Pecht, I. (1997) in Multicopper Oxidases (Messerschmidt, A., ed.) World Scientific, Singapore-New Jersey-London-Hong Kong, pp. 355–390.

    Google Scholar 

  6. Yaropolov, A. I., Skorobogat'ko, O. V., Vartanov, S. S., and Varfolomeyev, S. D. (1994) Appl. Biochem. Biotechnol., 49, 257–275.

    Google Scholar 

  7. Malmstrom, B. G. (1997) in Multicopper Oxidases (Messerschmidt, A., ed.) World Scientific, Singapore-New Jersey-London-Hong Kong, pp. 1–22.

    Google Scholar 

  8. Reinhammer, B. (1978) Biochim. Biophys. Acta, 534, 7–14.

    Google Scholar 

  9. Hanna, P. M., McMillin, D. R., Pasenkiewicz-Gierula, M., Antholine, W. E., and Reinhammar, B. (1988) Biochem. J., 253, 561–568.

    Google Scholar 

  10. Xu, F., Shin, W., Brown, S. H., Wahleithner, J. A., Sundaram, U. M., and Solomon, E. I. (1996) Biochim. Biophys. Acta, 1292, 303–311.

    Google Scholar 

  11. Koroleva-Skorobogat'ko, O., Stepanova, E., Gavrilova, V., Morozova, O., Lubimova, N., Dzchafarova, A., Yaropolov, A., and Makower, A. (1998) J. Biotechnol. Appl. Biochem., 28, 47–54.

    Google Scholar 

  12. Westermeier, R. (1993) Electrophoresis in Practice, VCH Verlagsgesellschaft, Weinheim and VCH Publishers Inc., NewYork, p. 277.

    Google Scholar 

  13. Conant, J. B., and Pratt, M. F. (1927) J. Am. Chem. Soc., 48, 3178–3220.

    Google Scholar 

  14. Gertsog, S., Gustav, K., and Shtrele, I. (1985) in Inorganic Synthesis Guide (Brower, G., ed.) [Russian translation], Mir, Moscow, pp. 1653–1654.

  15. Shleev, S. V., Kuznetsov, S. V., and Topunov, A. F. (2000) Prikl. Biokhim. Mikrobiol., 36, 354–358.

    Google Scholar 

  16. Karyakin, Yu. V., and Angelov, I. I. (1974) in Pure Chemical Compounds [in Russian], Khimiya, Moscow, pp. 20–21.

    Google Scholar 

  17. Koroleva (Skorobogat'ko), O., Stepanova, E., Gavrilova, V., Biniukov, V., Jaropolov, A., Varfolomeyev, S., Scheller, F., Makower, A., and Otto, A. (1999) J. Biotechnol. Appl. Biochem., 76, 115–118.

    Google Scholar 

  18. Salas, C., Lobos, S., Larrain, J., Salas, L., Cullen, D., and Vicuna, R. (1995) J. Biotechnol. Appl. Biochem., 21, 323–333.

    Google Scholar 

  19. Coll, P. M., Ternandez-Abalos, J. M., Villanueva, J. R., Santamaria, R., and Perez, P. (1993) Appl. Environ. Microbiol., 59, No. 8, 2607–2613.

    Google Scholar 

  20. Nishizawa, Y., Nakabayashi, K., and Shinagawa, E. (1995) J. Ferment. Bioeng., 80, 91–93.

    Google Scholar 

  21. Vaitkyavichus, R. K., Vel'zhite, V. A., and Chenas, N. K. (1984) Biokhimiya, 49, 1000–1003.

    Google Scholar 

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Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, Moscow, 117071, Russia

    O. V. Koroleva, I. S. Yavmetdinov, S. V. Shleev & E. V. Stepanova

  2. Komarov Botanical Institute, Russian Academy of Sciences, ul. Prof. Popova 2, St. Petersburg, 197022, Russia

    V. P. Gavrilova

Authors
  1. O. V. Koroleva
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  2. V. P. Gavrilova
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  3. I. S. Yavmetdinov
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  4. S. V. Shleev
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  5. E. V. Stepanova
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Koroleva, O.V., Gavrilova, V.P., Yavmetdinov, I.S. et al. Isolation and Study of Some Properties of Laccase from the Basidiomycetes Cerrena maxima. Biochemistry (Moscow) 66, 618–622 (2001). https://doi.org/10.1023/A:1010299012591

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  • DOI: https://doi.org/10.1023/A:1010299012591

  • Cerrena maxima
  • laccase
  • redox potential
  • pH optimum
  • isoelectric point
  • Michaelis constant