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Exploring the Properties of Thermostable Clostridium thermocellum Cellulase CelE for the Purpose of Its Expression in Plants

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Abstract

The main properties (pH and temperature range, stability, substrate specificity) of the modified cellulase CelE (endo-β-1,4-glucanase) from Clostridium thermocellum have been analyzed with the goal of its expression in plants. The modified enzyme is similar to plant cellulases. Deletions in the N-terminus of the enzyme do not affect its biochemical properties. Based on the present investigation, we conclude that the modified β-1,4-glucanase CelEM1, when expressed in plants, will be a good model to study the role of cellulases in plants.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, Moscow, 119991, Russia

    R. M. Abdeev, I. V. Goldenkova, K. A. Musiychuk & E. S. Piruzian

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  1. R. M. Abdeev
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  2. I. V. Goldenkova
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  3. K. A. Musiychuk
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  4. E. S. Piruzian
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Abdeev, R.M., Goldenkova, I.V., Musiychuk, K.A. et al. Exploring the Properties of Thermostable Clostridium thermocellum Cellulase CelE for the Purpose of Its Expression in Plants. Biochemistry (Moscow) 66, 808–813 (2001). https://doi.org/10.1023/A:1010277115744

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