Abstract
An intraperitoneal injection of an exogenous delta-sleep inducing peptide (DSIP) at a dose of 12 μg/100 g body weight shifted the prooxidant–antioxidant balance of free radical process (FRP) in tissues and erythrocytes of rats: the activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) and the concentrations of antioxidants (reduced glutathione in particular) increased. The DSIP stimulated the myeloperoxidase activity in blood neutrophils and had no effect on the activity of xanthine oxidase, a prooxidant enzyme, in the brain and liver. Cold stress displaced the prooxidant–antioxidant balance by increasing the xanthine oxidase activity in tissues and decreasing the myeloperoxidase activity in blood neutrophils; it also inhibited the enzyme antioxidant activities in tissues and erythrocytes that was neutralized by an increased ceruloplasmin activity in blood plasma and by an elevated level of antioxidants in rat blood and tissues. Preliminary administration of DSIP to animals exposed to cold stress restored the prooxidant–antioxidant balance: it normalized the myeloperoxidase activity in blood neutrophils, decreased the xanthine oxidase activity, and increased the activity of antioxidant enzymes in tissues and erythrocytes restoring the antioxidant level. The molecular regulation mechanism of free radical processes by DSIP in tissues under stressful conditions is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Men'shikova, E. B., and Zenkov, N. K. (1993) Usp. Sovr. Biol., 113, 442–445.
Men'shikova, E. B., and Zenkov, N. K. (1997) Usp. Sovr. Biol., 117, 155–171.
Monnier, M., and Schoenenberger, G. A. (1977) in 3rd Eur. Congr. Sleep. Res. Monpellier, 1976, Sleep. Basel, Karger, pp. 257–263.
Prudchenko, I. A., and Mikhaleva, I. I. (1994) Usp. Sovr. Biol., 114, 728–740.
Nekrasov, A. N., and Mikhaleva, I. I. (1996) Biochem. Mol. Biol. Int., 38, 739–745.
Koplik, E. V., Vedyaev, D. F., Mikhaleva, I. I., Sargsyan, A. S., Ivanov, V. T., and Sudakov, K. V. (1982) Dokl. Akad. Nauk SSSR, 267, 230–232.
Bondarenko, T. I., Krichevskaya, A. A., Krupennikova, E. Yu., and Mikhaleva, I. I. (1985) Fiziol. Zh. SSSR, 71, 279–282.
Mendzheritskii, A. M., Makletsova, M. G., and Karpukhina, I. Yu. (1987) Neirokhimiya, 6, 422–425.
Sudakov, K. V. (1991) Ross. Fiziol. Zh., 77, 1–13.
Khvatova, E. M., Gainullin, M. R., and Mikhaleva, I. I. (1995) Byull. Eksp. Biol. Med., 2, 141–143.
Ashmarin, I. P. (1988) Patol. Fiziol., 3, 3–8.
Ashmarin, I. P., and Obukhova, M. F. (1994) Vestnik RAMN, 10, 28–34.
Gomazkov, O. A. (1992) Functional Biochemistry of Peptide Regulators [in Russian], Nauka, Moscow.
Shcheglova, A. I. (1969) Physiology of Adaptation to Heat and Cold [in Russian], Nauka, Leningrad.
Ardashev, A. A., Semikina, V. V., and Mosin, A. F. (1978) Neuroendocrine Correlations [in Russian], DVNTs VSh, Vladivostok.
Maistrakh, E. V. (1979) Fiziol. Zh. SSSR, 65, 1582–1591.
Bondarenko, T. I., and Mikhaleva, I. I. (1992) in Delta-Sleep Inducing Peptide. Theoretical and Applied Aspects, Workshop, Rostov-on-Don, pp. 6–7.
Kastin, A. J., Olson, G. A., Schally, A. V., and Coy, D. A. (1980) Trends Neurol. Sci., 3, 163–165.
Graf, M. V., Lorez, H. P., Gillessen, D., Tobler, H. I., and Schoenenberger, G. A. (1981) Experientia, 37, 625–627.
Graf, M. V., and Kastin, A. J. (1984) Proc. Soc. Biol. Med., 177, 197–204.
Bondarenko, T. I. (1990) Metabolism of Homocarnosine in the Brain of Animals of Different Age under Extreme Environmental Conditions: Doctoral dissertation [in Russian], Rostov-on-Don.
Boyum, A. (1968) Scand. J. Clin. Invest., 21, 77–89.
Bligh, E., and Dyer, W. G. (1959) Can. J. Biochem. Physiol., 37, 911–917.
Kolb, V. G., and Kamyshnikov, V. S. (1982) Handbook of Clinical Chemistry [in Russian], Belarus', Minsk.
Avis, P. G., Bergel, F., and Bray, R. C. (1955) J. Chem. Soc., 4, 1100–1212.
Saidov, M. Z., and Pinegin, B. V. (1991) Lab. Delo, 3, 56–60.
Shustanova, T. A. (1999) Regulation by Delta-Sleep Inducing Peptide of Free Radical Processes in Tissues and Membranes of Rat Erythrocytes under Cold Stress: Candidate.s dissertation [in Russian], Rostov-on-Don.
Lowry, O. H., Rosenbrough, N. I., Farr, A. L., and Randall, R. J. (1951) J. Biol. Chem., 193, 265–275.
Fried, R. (1975) Biochem., 57, 657–660.
Korolyuk, M. A., Ivanova, L. I., Maiorova, I. G., and Tokarev, V. E. (1988) Lab. Delo, 1, 16–19.
Moin, V. M. (1986) Lab. Delo, 12, 724–727.
Yusupova, L. B. (1989) Lab. Delo, 4, 19–21.
Ellman, G. L. (1959) Arch. Biochem. Biophys., 82, 70–77.
Kornienko, I. V. (1997) Lipid Peroxidation under Experimental Salmonellosis: Candidate.s dissertation [in Russian], Rostov-on-Don.
Karakashov, A. V., and Vichev, E. P. (1973) Micromethods in Clinical Laboratory, Meditsina i Fizkul'tura, Sofia.
Gabrielyan, N. I., and Lipatova, V. I. (1984) Lab. Delo, 3, 138–140.
Davies, K. I. A. (1987) J. Biol. Chem., 262, 9895–9901.
Meerson, F. Z., and Pshennikova, M. G. (1988) Adaptation to Stressful Conditions and Physical Stress [in Russian], Meditsina, Moscow.
Meerson, F. Z., and Malyshev, I. Yu. (1993) Phenomenon of Structure Adaptive Stabilization and Heart Protection[in Russian], Nauka, Moscow.
Rikhireva, G. T., Makletsova, M. G., Mendzheritskii, A. M., Vartanyan, L. S., Gurevich, S. M., and Lozovskaya, E. L. (1993) Izv. RAN. Ser. Biol., 2, 243–255.
Krupennikova, E. Yu., Yukhanova, R. Yu., Mikhaleva, I. I., and Bondarenko, T. I. (1986) Neirokhimiya, 5, 190–193.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shustanova, T.A., Bondarenko, T.I., Milyutina, N.P. et al. Regulation of Free Radical Processes by Delta-Sleep Inducing Peptide in Rat Tissues under Cold Stress. Biochemistry (Moscow) 66, 632–639 (2001). https://doi.org/10.1023/A:1010255230338
Issue Date:
DOI: https://doi.org/10.1023/A:1010255230338