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Use of Random Amplified Polymorphic DNA Markers for Mapping the Chickpea Genome

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Biologia Plantarum

Abstract

Three interspecific crosses were developed using Cicer arietinum (ICC 4918) as the female parent and wild Cicer species [C. reticulatum - JM 2100, JM 2106 and C. echinospermum - ICCW 44] as the male parent. Cicer arietinum (ICC 4918) × C. reticulatum (JM 2100) cross produced the largest number of F2 plants and was chosen for linkage mapping using Random Amplified Polymorphic DNA (RAPD) primers. A partial linkage map was constructed based upon the segregation of 36 RAPD markers obtained by amplification using 35 primers. The linkage map consists of two linkage groups with 17 linked markers covering a total of 464.9 cM. Analyses also revealed association of three morphological traits with linked RAPD markers. Out of seven morphological traits tested for association with linked markers in the segregating plants, four Quantitative trait loci (QTL) were detected for the trait leaf length and three QTLs each for the traits leaf width and erect plant habit.

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References

  • Ahmad, F., Gaur, P.M., Slinkard, A.E.: Isozyme polymorphism and phylogenetic interpretations in genus Cicer L.-Theor. appl. Genet. 83: 620–627, 1992.

    Google Scholar 

  • Banerjee, H., Pai, R.A., Sharma, R.P.: Restriction fragment length polymorphism and random amplified polymorphic DNA analysis of chickpea accessions.-Biol. Plant. 42: 197–208, 1999.

    Google Scholar 

  • Carlson, J.E., Tulseiram, L.K., Glaubitz, J.C., Luk, V.W.K., Kauffeldt, C., Rutledge, R.: Segregation of random amplified DNA markers in F1 progeny of conifers.-Theor. appl. Genet. 83: 194–200, 1991.

    Google Scholar 

  • Doyle, J.J., Doyle, J.L.: A rapid isolation procedure for small quantities of fresh leaf tissue.-Phytochem. Bull. 19: 11–15, 1987.

    Google Scholar 

  • Echt, C.S., Erdahl, L.A., McCoy, T.J.: Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa.-Genome 35: 84–87, 1992.

    Google Scholar 

  • Gaur, P.M., Slinkard, A.E.: Genetic control and linkage relations of additional isozyme markers in chickpea.-Theor. appl. Genet. 80: 648–656, 1990a.

    Google Scholar 

  • Gaur, P.M., Slinkard, A.E.: Inheritance and linkage of isozyme coding genes in chickpea.-J. Hered. 81: 455–461, 1990b.

    Google Scholar 

  • Gaur, P.M., Slinkard, A.E.: Duplication of the structural gene for the plastid specific isozyme of aldolase in Cicer.-Genome 34: 151–155, 1991.

    Google Scholar 

  • Jermstad, K.D., Reem, A.M., Henifin, J.R., Wheeler, N.C., Neale, D.B.: Inheritance of restriction fragment length polymorphism and random amplified polymorphic DNA in coastal Douglas-fir.-Theor. appl. Genet. 89: 758–766, 1994.

    Google Scholar 

  • Kazan, K., Muehlbauer, F.J.: Allozyme variation and phylogeny in annual species of Cicer (Leguminosae).-Plant Syst. Evol. 175: 11–21, 1991.

    Google Scholar 

  • Kazan, K., Muehlabuer, F.J., Weeden, N.W., Ladizinsky, G.: Inheritance and linkage relationships of morphological and isozyme loci in chickpea (Cicer arietinum L.).-Theor. appl. Genet. 86: 417–426, 1993.

    Google Scholar 

  • Kesseli, R.V., Paran, I., Michelmore, M.W.: Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers.-Genetics 136: 1435–1446, 1994.

    Google Scholar 

  • Kiss, G.B., Cassandi, G., Kalman, K., Kalo, P., Okresz, L.: Construction of a basic genetic map for alfalfa using RFLP, RAPD, isozyme and morphological markers.-Mol. gen. Genet. 238: 129–137, 1993.

    Google Scholar 

  • Kosambi, D.D.: The estimation of map distance from recombination values.-Ann. Eugenet. 12: 172–175, 1944.

    Google Scholar 

  • Ladizinsky, G., Adler, A.: The origin of chickpea (Cicer arietinum L.).-Euphytica 25: 211–217, 1976a.

    Google Scholar 

  • Ladizinsky, G., Adler, A.: Genetic relationships among the annual species of Cicer L.-Theor. appl. Genet. 48: 197–203, 1976b.

    Google Scholar 

  • Lander, E.S., Green, P., Abrahamson, J., Barlow, A., Daly, M.J., Lincoln, S.E., Newberg, L.: MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental natural populations.-Genomics 1: 174–181, 1987.

    Google Scholar 

  • Lander, E.S., Botstein, D.: Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps.-Genetics 121: 185–199, 1989.

    Google Scholar 

  • Litt, M., Luty, J. A.: A hypervariable microsatellite revealed by in-vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene.-Amer. J. Human Genet. 44: 397–401, 1989.

    Google Scholar 

  • Muehlbauer, F.J., Singh, K.B.: Genetics chickpea.-In: Saxena, M.C., Singh, K.B. (ed.): The Chickpea. Pp. 99–126. CAB Int. Publication, Oxfordshire 1987.

    Google Scholar 

  • Ohri, D., Pal, M.: Origin of chickpea: karyotype and nuclear DNA content.-Heredity 66: 367–372, 1991.

    Google Scholar 

  • Saghai-Maroof, M., Soliman, K., Jorgenson, R., Allard, R.: Ribosomal DNA spacer length polymorphism in barley and its Mendelian inheritance.-Proc. nat. Acad. Sci. USA 81: 8014–8018, 1984.

    Google Scholar 

  • Sharma, P.C., Winter, P., Bunger, T., Huttel, B., Weigand, F., Weising, K., Kahl, G.: Abundance of polymorphism of di-, tri-and tetranucleotide tandem repeats in chickpea (Cicer arietinum L.).-Theor. appl. Genet. 90: 90–96, 1995.

    Google Scholar 

  • Sheila, V.K., Moss, J.P., Gowda, C.L.L., van Rheenen, H.A.: Interspecific hybridisation between Cicer arietinum and wild Cicer species.-Int. Chickpea Newslett. 27: 11–13, 1992.

    Google Scholar 

  • Singh, K.B., Holly, L., Bejiga, G.: A Catalogue of Kabuli Chickpea Germplasm (an evaluation report of winter-sown kabuli chickpea land races, breeding lines and wild Cicer species).-ICARDA, Aleppo 1991.

    Google Scholar 

  • Singh, K.B., Saxena, M.C.: Sources of resistance in cultigen to biotic and abiotic stresses identified between 1978 and 1992.-ICARDA Annu. Rep. 1992. Pp. 23–25, 1992.

  • Strickberger, M.W.: Genetics. 3rd Edition.-Macmillan, New York 1985.

    Google Scholar 

  • Tanksley, S.D., Young, N.D., Paterson, A.: RFLP mapping in plant breeding: new tools for an old science.-Bio/Technology 7: 257–264, 1989.

    Google Scholar 

  • Tayyar, R.J., Waines, J.G.: Genetic relationships among annual species of Cicer (Fabaceae) using isozyme variation.-Theor. appl. Genet. 92: 245–254, 1996.

    Google Scholar 

  • Udupa, S.M., Sharma, A., Sharma, R.P., Pai, R.A.: Narrow genetic variability in (Cicer arietinum L.). as revealed by RFLP analysis.-J. Plant Biochem. Biotechnol. 2: 84–86, 1993.

    Google Scholar 

  • Van Rheenen, H.A.: Biotechnology and chickpea breeding.-Int. Chickpea Newslett. 26: 14–17, 1992.

    Google Scholar 

  • Weising, K., Kaemmer, D., Weigand, F., Epplen, J.T., Kahl, G.: Oligonucleotide fingerprinting reveals various probe-dependent levels of informativeness in chickpea (Cicer arietinum L.).-Genome 35: 436–442, 1992.

    Google Scholar 

  • Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V.: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.-Nucl. Acids Res. 18: 6531–6535, 1990.

    Google Scholar 

  • Winter, P., Kahl, G.: Molecular technologies for plant improvement.-World J. Microbiol. Biotechnol. 11: 438–448, 1995.

    Google Scholar 

  • Winter, P., Pfaff, T., Udupa, S.M., Huttel, B., Sharma, P.C., Sahi, S., Arreguin-Espinoza, R., Weigand, F., Muehlbauer, F.J., Kahl, G.: Characterization and mapping of sequencetagged microsatellite sites in the chickpea (Cicer arietinum L.) genome.-Mol. gen. Genet. 262: 90–101, 1999.

    Google Scholar 

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Banerjee, H., Pai, R., Moss, J. et al. Use of Random Amplified Polymorphic DNA Markers for Mapping the Chickpea Genome. Biologia Plantarum 44, 195–202 (2001). https://doi.org/10.1023/A:1010234922360

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