Skip to main content
SpringerLink
Log in

Transcriptional and functional analysis of the gene for factor C, an extracellular signal protein involved in cytodifferentiation of Streptomyces griseus

  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Factor C is an extracellular signal protein involved in cellular differentiation in Streptomyces griseus. Nuclease S1 mapping experiments revealed that transcription of the gene takes place from a single promoter in a developmental-stage specific manner. The latter was also confirmed by in vivo promoter probing. The sequence of its promoter suggests that the gene is not transcribed by the major sigma factor. The cloned gene expressed from its own promoter in low- and high-copy-number vectors restored normal sporulation to a bald mutant of Streptomyces griseus. Computer analysis of the amino acid sequence revealed the presence of a transmembrane localization segment with the N-terminus positioned inside the cell. These data fit well into our working model that points at an important role for factor C in the morphogenesis of Streptomyces griseus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Babcock MJ & Kendrick KE (1988) Cloning of DNA involved in sporulation of Streptomyces griseus. J. Bacteriol. 170: 2802–2808

    Google Scholar 

  • Birkó Zs, Sümegi A, Vinnai A, van Wezel GP, Szeszák F, Vitális S, Szabó P, Kele Z, Janáky T & Biró S (1999) Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus. Microbiol. 145: 2245–2253

    Google Scholar 

  • Biró S, Békési I, Vitális S & Szabó G (1980) A substance effecting differentiation in Streptomyces griseus. Eur. J. Biochem. 103: 359–363

    Google Scholar 

  • Brown KL, Wood S & Buttner MJ (1992) Isolation and characterization of the major vegetative RNA polymerase of Streptomyces coelicolor A3(2); renaturation of a sigma subunit using GroEL. Mol. Microbiol. 6: 1133–1139

    Google Scholar 

  • Chater KF (1989) Multilevel regulation of Streptomyces differentiation. Trends in Genetics 5: 372–377

    Google Scholar 

  • Chater KF (1998) Taking a genetic scalpel to the Streptomyces colony. Microbiol. 144: 1465–1478

    Google Scholar 

  • Cserzo M, Wallin E, Simon I, Von Heijne G & Elofsson A (1997) Prediction of transmembrane alpha helices in prokaryotic membrane proteins: the Dense Alignment Surface method. Prot. Eng. 10(6): 673–676

    Google Scholar 

  • Floriano B & Bibb MJ (1996) afsR is a pleiotropic but conditionally required regulatory gene for antibiotic production in Streptomyces coelicolor A3(2). Mol. Microbiol. 21: 385–396

    Google Scholar 

  • Hawley DK & McClure WR (1983) Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 11: 2237–2255

    Google Scholar 

  • Hofmann K & Stoffel W(1993) Tmbase-A database of membrane spanning proteins segments. Biol. Chem. Hoppe-Seyler 347: 166

    Google Scholar 

  • Hopwood DA (1999) Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico. Microbiol. 145: 2183–2202

    Google Scholar 

  • Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM & Schrempf H (1985) Genetic manipulation of Streptomyces: a laboratory manual. Norwich: John Innes Foundation.

    Google Scholar 

  • Horinouchi S & Beppu T (1992) Autoregulatory factors and communication in actinomycetes. Annu Rev Microbiol. 46: 377–398

    Google Scholar 

  • Horinouchi S (1996) Streptomyces genes involved in aerial mycelium formation. FEMS Microbiol Lett. 141: 1–9

    Google Scholar 

  • Janssen GR & Bibb MJ (1993) Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene 124: 133–134

    Google Scholar 

  • Khokhlov AS (1991) Microbial Autoregulators. Harwood Academic Publishers, Chur-Reading-Paris-Philadelphia-Tokyo-Melbourne.

    Google Scholar 

  • Kudo N, Kimura M, Beppu T & Horinouchi S (1995) Cloning and characterization of a gene involved in aerial mycelium formation in Streptomyces griseus. J. Bacteriol. 177: 6401–6410

    Google Scholar 

  • Larson JL & Herschberger CL (1986) The minimal replicon of a streptomycete plasmid produces an ultrahigh level of plasmid DNA. Plasmid 15: 199–209

    Google Scholar 

  • MacNeil DJ, Gewain KM, Ruby CL, Dezeny G, Gibbons PH & MacNeil T (1992) Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilising a novel integration vector. Gene 111: 1–68

    Google Scholar 

  • Messing J, Crea R & Seeburg PH (1981) A system for shotgun DNA sequencing. Nucleic Acids Res. 9: 309–321

    Google Scholar 

  • Murray MG (1986) Use of sodium trichloroacetate and mung bean nuclease to increase sensitivity and precision during transcript mapping. Anal. Biochem. 158: 165–170

    Google Scholar 

  • Narva KE & Feitelson JS (1990) Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2). J. Bacteriol. 172: 326–333

    Google Scholar 

  • Strauch E, Takano E, Baylis HA & Bibb MJ (1991) The stringent response in Streptomyces coelicolor A3(2). Mol. Microbiol. 5: 289–298

    Google Scholar 

  • Strohl WR (1992) Compilation and analysis of DNA sequences associated with apparent streptomycete promoters. Nucleic Acids Res. 20(5): 961–974

    Google Scholar 

  • Strohl WR (1997) Industrial antibiotics: Today and the future. In: Strohl, WR (Ed) Biotechnology of antibiotics (pp. 1–47). Marcel Dekker, Inc., New York-Basel-Hong Kong.

    Google Scholar 

  • Szabó G, Vályi-Nagy T & Vitális S (1962) A new factor regulating life cycle of Streptomyces griseus. In: Timakova VD (Ed) Genetics ofMicroorganisms, Proceedings of a Symposium on Heredity and Variability of Microorganisms. State Publishing House on Medical Literature, Moscow.

    Google Scholar 

  • Szabó PT, Kele Z, Birkó Zs, Szeszák, F, Biró S & Janáky T (1999) Identification of factor C protein from Streptomyces griseus by microelectrospray mass spectrometry. J. Mass Spectrometry 34: 1312–1316

    Google Scholar 

  • Szeszák F, Vitális S & Szabó G (1991) Presence of factor C in streptomycetes and other bacteria. In: Baumberg S, Krügel H & Noack D (Eds) Genetics and product formation in Streptomyces. Plenum Press, New York-London.

    Google Scholar 

  • Ueda K, Miyake K, Horinouchi S & Beppu T (1993) A gene cluster involved in aerial mycelium formation in Streptomyces griseus encodes proteins similar to the response regulators of two-component regulatory systems and membrane translocators. J. Bacteriol. 175: 2006–2016

    Google Scholar 

  • Vara J, Lewandowska-Skarbek M, Wang Y-G, Donadio S & Hutchinson CR (1989) Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus). J. Bacteriol. 171: 5872–5881

    Google Scholar 

  • Vitális S, Szabó G & Vályi-Nagy T (1963) Comparison of streptomycin-producing and non-producing strains of Streptomyces griseus. Acta Biol. Acad. Sci. Hung. 14: 1–15

    Google Scholar 

  • Vitális S & Szabó G (1969) Cytomorphological effect of factor C in submerged cultures on the hyphae of Streptomyces griseus strain No. 52–1. Acta Biol. Acad. Sci. Hung. 20: 85–92

    Google Scholar 

  • van Wezel GP,White J, Hoogvliet G & Bibb MJ (2000) Application of redD, the transcriptional activator gene of the undecylprodigiosin biosynthetic pathway, as a reporter for transcriptional activity in Streptomyces coelicolor A3(2) and Streptomyces lividans. J Mol. Microbiol. Biotechnol (In press).

  • Yamada Y, Nihira T & Sakuda S (1997) Butyrolactone autoregulators, inducers of virginiamycin in Streptomyces virginiae: Their structures, biosynthesis, receptor proteins, and induction of virginiamycin biosynthesis. In: Strohl, WR (Ed) Biotechnology of antibiotics (pp. 63–79). Marcel Dekker, Inc., New York-Basel-Hong Kong.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Human Genetics, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, H-4012, Debrecen, Nagyerdei körút 98, Hungary

    Sándor Biró & Zsuzsanna Birkó

  2. Department of Biochemistry, Leiden University, Gorlaeus Laboratory, P.O. Box 9502, 2300, RA Leiden, The Netherlands

    Gilles P. van Wezel

Authors
  1. Sándor Biró
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zsuzsanna Birkó
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gilles P. van Wezel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sándor Biró.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Biró, S., Birkó, Z. & van Wezel, G.P. Transcriptional and functional analysis of the gene for factor C, an extracellular signal protein involved in cytodifferentiation of Streptomyces griseus . Antonie Van Leeuwenhoek 78, 277–285 (2000). https://doi.org/10.1023/A:1010221407340

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1010221407340

Search

Navigation