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A Fast Method to Estimate Kinetic Constants for Enzyme Inhibitors

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Abstract

We present a method to determine the reaction type and kinetic constants for enzyme inhibitors that decreases the number of experimental assays by at least a factor of five. It is based on a new theoretical formalism in terms of concentrations that dismisses the requirement of estimating initial velocities. Expressions for the time evolution of the concentrations of all the reactants are also given.

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Authors and Affiliations

  1. Centre for Mathematical Biology, Mathematical Institute, 24-29 St Giles', Oxford, OX1 3LB, UK

    S. Schnell

  2. Centro de Física, Instituto Venezolano de Investigaciones Científicas (IVIC), PO Box 21827, Caracas, 1020A, Venezuela

    C. Mendoza

Authors
  1. S. Schnell
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  2. C. Mendoza
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Schnell, S., Mendoza, C. A Fast Method to Estimate Kinetic Constants for Enzyme Inhibitors. Acta Biotheor 49, 109–113 (2001). https://doi.org/10.1023/A:1010219527831

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