Abstract
Muconate cycloisomerase (MCI) was purified from Rhodococcus rhodochrous 89 grown on phenol. The enzyme appears to contain two different type subunits with molecular masses 35.5 and 37 kD. The N-terminal amino acid sequence of both subunits showed more similarity to corresponding enzymes from gram-negative bacteria than to one from Rhodococcus opacus 1CP. MCI from R. rhodochrous 89, like analogous enzymes from gram-negative bacteria, can convert 2-chloromuconate (2-CM) with the formation of both, 2- and 5-chloromuconolactones (CML) as intermediates. Nevertheless, its unique ability to convert 5-CML to cis- but not to trans-dienelactone sets it apart from all known chloromuconate cycloisomerases from gram-negative and gram-positive bacteria.
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Solyanikova, I.P., Schlömann, M. & Golovleva, L.A. A New Type of Muconate Cycloisomerase from Rhodococcus rhodochrous Strain 89. Biochemistry (Moscow) 66, 747–752 (2001). https://doi.org/10.1023/A:1010208628039
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DOI: https://doi.org/10.1023/A:1010208628039