Skip to main content
SpringerLink
Log in

Effect of Solvent Composition on DSC Exothermic Peak of Human Serum Albumin Suspended in Pyridine-n-Hexane Mixtures

  • Published:
Journal of Thermal Analysis and Calorimetry Aims and scope Submit manuscript

Abstract

Human serum albumin (HSA) immersed in pyridine-n-hexane mixtures was analyzed using differential scanning calorimetry (DSC). State of the solid HSA in organic solvent mixtures is the non-equilibrium state which is seen as the exothermic peak on the DSC curves. The enthalpy change corresponding to this exothermic peak approaches zero when going from pure pyridine to pure n-hexane. Dependence of the enthalpy change on the pyridine concentration is suggestive that the non-equilibrium state of the immersed HSA results from the HSA-pyridine interactions 'frozen' at the lower temperature. Most likely the temperature-initiated exothermic peak observed on the DSC curves reflects the swelling of HSA by pyridine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. K. Martinek, A. V. Levashov, N. Klyachko, V. I. Pantin and I. V. Berezin, Biochim. Biophys. Acta, 657 (1981) 277.

    CAS  Google Scholar 

  2. K. Martinek, A. V. Levashov, N. Klyachko, Y. L. Khmelnitskiy and I. V. Berezin, Eur. J. Biochem., 155 (1986) 453.

    Article  CAS  Google Scholar 

  3. A. M. Klibanov, Trends Biochem. Sci., 14 (1989) 141.

    Article  CAS  Google Scholar 

  4. C. S. Chen and C. J. Sih, Angew. Chem., 101 (1989) 711.

    CAS  Google Scholar 

  5. A. M. Klibanov, Acc. Chem. Res., 23 (1990) 114.

    Article  CAS  Google Scholar 

  6. A. Zaks and A. M. Klibanov, Science, 224 (1984) 1249.

    CAS  Google Scholar 

  7. G. Ayala, M. T. Gomez-Puyou, A. Gomez-Puyou and A. Darszan, FEBS Lett., 203 (1986) 41.

    Article  CAS  Google Scholar 

  8. D. B. Volkin, A. Staubli, R. Langer and A. M. Klibanov, Biotechnol. Bioeng., 37 (1991) 843.

    Article  CAS  Google Scholar 

  9. P. L. Privalov, Adv. Prot. Chem., 33 (1979) 167.

    Article  CAS  Google Scholar 

  10. P. L. Privalov, Thermochim. Acta, 163 (1990) 33.

    Article  CAS  Google Scholar 

  11. P. L. Luisi, G. Haring, M. Maestro and G. Rialdi, Thermochim. Acta, 162 (1990) 1.

    Article  CAS  Google Scholar 

  12. E. Battistel, P. L. Luisi and G. Rialdi, J. Phys. Chem., 92 (1988) 6680.

    Article  CAS  Google Scholar 

  13. J. Fujita and Y. Noda, Int. J. Pept. Res., 18 (1984) 12.

    Article  Google Scholar 

  14. J. Fujita and Y. Noda, Bull. Chem. Soc. Jpn., 52 (1979) 2349.

    Article  CAS  Google Scholar 

  15. E. Battistel and D. Bianchi, J. Phys. Chem., 98 (1994) 5368.

    Article  CAS  Google Scholar 

  16. D. V. Zakharychev, M. D. Borisover and B. N. Solomonov, Russ. J. Phys. Chem. in English Translation, 69 (1995) 162.

    Google Scholar 

  17. D. D. Perrin, W. L. F. Armarego and D. R. Perrin, Purification of Laboratory Chemicals, Pergamon Press, Oxford 1980.

    Google Scholar 

  18. M. D. Borisover, V. A. Sirotkin and B. N. Solomonov, Russ. J. Phys. Chem. in English Translation, 68 (1994) 792.

    Google Scholar 

  19. M. D. Borisover, V. A. Sirotkin and B. N. Solomonov, Thermochim. Acta, 284 (1996) 263.

    Article  CAS  Google Scholar 

  20. V. A. Sirotkin, M. D. Borisover and B. V. Solomonov, Biophys. Chem., 69 (1997) 239.

    Article  CAS  Google Scholar 

  21. P. L. Poole and J. L. Finney, Int. J. Biol. Macromol., 5 (1983) 308.

    Article  CAS  Google Scholar 

  22. P. A. Burke, R. G. Griffin and A. M. Klibanov, Biotechnol. Bioeng., 42 (1993) 87.

    Article  CAS  Google Scholar 

  23. W. R. Liu, R. Langer and A. M. Klibanov, Biotechnol. Bioeng., 37 (1991) 177.

    Article  CAS  Google Scholar 

  24. G. M. Jordan, S. Yoshioka and R. Terao, J. Pharm. Pharmacol., 46 (1994) 182.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Soil, Water and Environmental Sciences, The Volcani Center, ARO, 50250, Bet Dagan, Israel

    M. D. Borisover

  2. Department of Chemistry, Kazan State University, 420008, Kazan, Russia

    D. V. Zakharychev & B. N. Solomonov

Authors
  1. M. D. Borisover
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. V. Zakharychev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. N. Solomonov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Borisover, M.D., Zakharychev, D.V. & Solomonov, B.N. Effect of Solvent Composition on DSC Exothermic Peak of Human Serum Albumin Suspended in Pyridine-n-Hexane Mixtures. Journal of Thermal Analysis and Calorimetry 55, 85–92 (1999). https://doi.org/10.1023/A:1010180004973

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1010180004973

Search

Navigation