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Metastable State of the Fc Fragment

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Abstract

The metastable state appearing at neutral pH in the Fc fragment after its brief incubation at pH 2.5 was studied using scanning microcalorimetry, high-flow sedimentation and fluorescence polarization. It was shown that the metastable state of the Fc fragment is characterised by smaller melting enthalpy of CH2 domains as compared with that of the stable state which is explained by reduced interaction of CH2 and CH3domains. As seen from the decrease in the sedimentation coefficient, this leads to diminution of compactness of the Fc fragment molecule. The intramolecular motility of CH2 domains increases judging by the fluorescence polarization data.

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Authors and Affiliations

  1. Institute of Immunological Engineering, 142380, Lyubuchany, Moscow Region

    V. M. Tischenko

  2. Institute of Biological Instrumentation, 142292, Pushchino, Moscow Region, pRussia

    V. M. Tischenko

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  1. V. M. Tischenko
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Tischenko, V.M. Metastable State of the Fc Fragment. Journal of Thermal Analysis and Calorimetry 62, 63–68 (2000). https://doi.org/10.1023/A:1010154510035

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  • DOI: https://doi.org/10.1023/A:1010154510035

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