Skip to main content
SpringerLink
Log in

DSC Studies of the Effects of Cisplatin and Transplatin on G-Actin

  • Published:
Journal of Thermal Analysis and Calorimetry Aims and scope Submit manuscript

Abstract

The effects of cisplatin and its trans isomer transplatin on the thermal denaturation of G-actin were studied with a Micro DSC-III differential scanning calorimeter. The denaturation enthalpy of G-actin was found to be 12 J g−1, and the denaturation temperature was 328 K. The thermal denaturation curve showed that increasing cisplatin concentration decreased the enthalpy change. However, after the ratio of cisplatin to G-actin attained 8:1 (mol:mol), the denaturation enthalpy no longer decreased. Transplatin decreased the enthalpy change more rapidly. In contrast with cisplatin, the denaturation peak at 328 K disappeared, and a strong exothermic peak appeared at 341 K when the ratio of transplatin to G-actin was 8:1 (mol:mol). The enthalpy change was 75 J g−1, which is far in excess of the range of weak interactions. This strong exothermic phenomenon probably reflects the agglutination of protein. The effects of cisplatin and transplatin on the number of the free thiol groups of G-actin are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. E. D. Korn, Physiol. Rev., 62 (1982) 672.

    Google Scholar 

  2. P. Sheterline and J. C. Sparrow, Protein Profile, 1 (1994) 1.

    Google Scholar 

  3. G. Chu, J. Biol. Chem., 269 (1994) 787.

    Google Scholar 

  4. C. A. Miller, M. D. Cohen and M. Costa, Carcinogenesis, 12 (1991) 269.

    Google Scholar 

  5. S. Luo, J. Tao and Q. He, Prog. Biochem. Biophys., 18 (1991) 200.

    Google Scholar 

  6. H. Zeng, B. Wang, Y. Zhang and K. Wang, Thermochim. Acta, 265 (1995) 31.

    Google Scholar 

  7. A. Bertazzon, G. H. Tian, A. Lamblin and T. Y. Tsong, Biochemistry, 29 (1990) 291.

    Google Scholar 

  8. J. D. Pardee and J. A. Spudich, Methods in Cell Biology, Academic Press, 1982, Vol. 24, p. 271.

  9. M. M. Bradford, Anal. Biochem., 72 (1976) 248.

    Google Scholar 

  10. G. L. Ellman, Archives of Biochemistry and Biophysics, 82 (1959) 70.

    Google Scholar 

  11. H. H. Zeng, Ph.D. Thesis, Beijing Medical University, 1994.

Download references

Author information

Authors and Affiliations

  1. Institute of Physical Chemistry, Peking University, Beijing, 100871, P. R. China

    X.-L. Zhou, Y. Yang, Z.-F. Li, B.-H. Wang & Y.-M. Zhang

Authors
  1. X.-L. Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Z.-F. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B.-H. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y.-M. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhou, XL., Yang, Y., Li, ZF. et al. DSC Studies of the Effects of Cisplatin and Transplatin on G-Actin. Journal of Thermal Analysis and Calorimetry 58, 243–248 (1999). https://doi.org/10.1023/A:1010143910861

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1010143910861

Search

Navigation