Journal of Nanoparticle Research

, Volume 2, Issue 2, pp 133–139 | Cite as

A Novel Approach for Introducing Bio-Materials Into Cells

  • Da-Ren Chen
  • Christine H. Wendt
  • David Y.H. Pui

Abstract

A novel approach was developed to introduce biological materials into cells for gene transfection and gene therapy applications. The method is based on the technique of electrospraying bio-materials into cells. A prototype apparatus was constructed for a feasibility study. The features of the gene transfector include: (1) A dual-capillary assembly to spray suspensions of biological materials. The outer capillary provided sheathing liquid that controlled the charge level on individual particles without altering the properties of suspensions. (2) An air–CO2 gas mixture was used for suppressing possible corona discharge and kept the same gas composition as those in incubators. (3) The designed chamber enabled the spray to operate at reduced pressure for increasing sprayed particle velocity. In the feasibility study, both suspensions of plasmid and plasmid-coated gold particles were used. The plasmid used was the commercially available Enhanced Green Fluorescent Protein gene. COS-1 cells were used as the target and the liquid media was evacuated immediately prior to the spraying process. Electrospraying was conducted at ambient pressure and the duration was no more than 2 min. After the spray transfection, the media was immediately replaced and the cell samples were returned to the incubator for 36 h. Transgene expression was detected by cellular fluorescence. This technology promises to have great potential for gene transfection and therapy studies.

electrospray of nanodroplets gene therapy gene transfection DNA transfer gold nanoparticles design of apparatus 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Da-Ren Chen
    • 1
  • Christine H. Wendt
    • 2
  • David Y.H. Pui
    • 3
  1. 1.Particle Technology Laboratory, Department of Mechanical EngineeringUniversity of MinnesotaMinneapolisUSA
  2. 2.Pulmonary and Critical Care, Department of Medicine, Medical SchoolUniversity of MinnesotaMinneapolisUSA
  3. 3.Particle Technology Laboratory, Department of Mechanical EngineeringUniversity of MinnesotaMinneapolisUSA

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