Skip to main content
Log in

DNA Analysis in Microfabricated Formats

  • Published:
Biomedical Microdevices Aims and scope Submit manuscript

Abstract

The use of microfabricated DNA analysis tools utilizing microfluidics will provide the next generation of inexpensive DNA diagnostics. It will also provide methodologies to measure gene expression in a massively parallel manner, eventually providing the methodologies to measure most or all the human genes of significance on a single chip. These technologies, including PCR analysis, electrophoresis and gene chips are described using examples from the archival literature.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. S. Abramowitz, Trends in Biotechnology 14, 397 (1996).

    Article  Google Scholar 

  2. F.S. Collins, A. Patrinos, E. Jordan, A. Chakravarti, R. Gesteland, and L. Waters, Science 282, 682 (1998).

    Article  Google Scholar 

  3. A listing of all sequenced genomes and references can be found in the TIGR home page: www.tigr.org.

  4. R. Waterston and J.E. Sulston, Science 282, 53 (1998).

    Article  Google Scholar 

  5. J.C. Venter, M.D. Adams, G.G. Sutton, A.R. Kerlavage, H.O. Smith, and M. Hunkapiller, Science 280, 1540 (1998).

    Article  Google Scholar 

  6. F.C. Collins, M.S. Guyer, and A. Chakravarti, Science 278, 1580 (1998).

    Article  Google Scholar 

  7. C.A. Dahl and R.L. Strausberg, SPIE 190, 3680 (1996).

    Google Scholar 

  8. The use of these technologies in the analysis of cancer genomics may be found on the National Cancer Institute Cancer Genome Anatomy Project website: www.nlm.nih.gov/ncicgap.

  9. K.E. Petersen, W.A. McMillan, T.A. Kovacs, M.A. Northup, and L.A. Christel, Biomedical Devices 1, 71 (1998).

    Google Scholar 

  10. A. Van den Berg and T.S.J. Lammerink, Micro Total Analysis Systems: Microfluidic Aspects, Integration Concept and Applications in Topics in Current Chemistry 194, 21, A. Manz, H. Becker, Ed. (Springer-Verlag, Heidelberg, Germany, 1998).

    Google Scholar 

  11. D. Qin, Y. Xia, J.A. Rogers, R.J. Jackman, X-M. Zhao, and G.M. Whitesides, Microfabrication, Microstructure and Microsystems, in Current Chemistry 194, 117, A. Manz, H. Becker, Ed. (Springer Verlag Heidelberg, Germany, 1998), 1.

    Google Scholar 

  12. M.S. Ibrahim, R.S. Loftis, P.B. Jahrling, E.A. Henschal, V.W. Weedn, M.A. Northrup, and P. Belgrader, Anal. Chem. 70, 2013 (1998).

    Article  Google Scholar 

  13. M.A. Northrup, B. Bennet, D. Hadley, P. Landre, S. Lehew, J. Richards, and P. Stratton, Anal. Chem. 70, 918 (1998).

    Article  Google Scholar 

  14. M.A. Northrup, B. Beeman, B. Benett, D. Hadley, P. Landre, and Lehew, A Miniature Integrated Nucleic Acid Analysis System, in Automation Technologies for Genome Characterization T.J. Beugelsdijk, Ed. (John Wiley & Sons, New York, 1997).

    Google Scholar 

  15. P. Belgrader, J.K. Smith, V.W. Weedn, and M.A. Northrup, J. Forensic Sc. 43, 315 (1998).

    Google Scholar 

  16. S. Harvey, M. Albin, L. Lebak, I. Mowatt, T. Schuerlin, and E. Principe, Biomedical Devices 1, 65 (1998).

    Google Scholar 

  17. T.M. Woudenberg, E.S. Winn-Dean, and M. Albin, High Density PCR and Beyond, Analytical Methods and Instrumentation, Special Issue µTAS '96 55 (1996).

  18. T.B. Taylor, E.S. Winn-Deen, E. Picozza, T.M. Woudenberg, and M. Albin, Nucleic Research 25, 3164 (1997).

    Article  Google Scholar 

  19. J. Cheng, L.C. Waters, P. Fortina, G. Hvichia, S.C. Jacobson, J.M. Ramsey, L.J. Kricka, and P. Wilding, Anal. Biochem. 257, 101 (1998).

    Article  Google Scholar 

  20. M.U. Kopp, A.J. deMello, and A. Manz, Science 280, 1046 (1998).

    Article  Google Scholar 

  21. P.C. Simpson, A.T. Wooley, and R.A. Mathies, Biomedical Devices 1, 7 (1998).

    Google Scholar 

  22. C.S. Effenhauser, Integrated Chip-Based Microcolumn Separation Systems in Topics in Current Chemistry 194, 51, A. Manz, H. Becker, Ed. (Springer-Verlag, Heidelberg, Germany, 1998).

    Google Scholar 

  23. A.T. Wooley and R.A. Mathies, Anal. Chem. 67, 3676 (1995).

    Article  Google Scholar 

  24. R.M. McCormick and R.J. Nelson, M.G. Alonso-Amigo, D.J. Benvegnu, and H.H. Hooper, Anal. Chem. 69, 2626 (1997).

    Article  Google Scholar 

  25. A.T. Wooley, D. Hadley, P. Landre, A. deMello, and R.A. Mathies, Anal. Chem. 68, 4081 (1996).

    Article  Google Scholar 

  26. P.C. Simpson, D. Roach, A.T. Wooley, T. Thorsen, R. Johnston, G.F. Sensabaugh, and R.A. Mathies, Proc. Natl. Acad. Sci. (USA) 95, 2256 (1998).

    Article  Google Scholar 

  27. C.H. Li and D.J. Harrison, Anal. Chem. 69, 1564 (1997).

    Article  Google Scholar 

  28. S.C. Jacobson and J.M. Ramsey, Anal. Chem. 68, 720 (1996).

    Article  Google Scholar 

  29. M. Schena, R.A. Heller, T.P. Theriault, K. Konrad, E. Lachenmeier, and R.W. Davis, Trends in Biotechnology 16, 301 (1998).

    Article  Google Scholar 

  30. R.A. Heller, M. Schena, A. Chai, D. Shalon, T. Bedilion, J. Gilmore, D.E. Wooley, and R.W. Davis, Proc. Nat. Acad. Sci., (USA) 94, 2150 (1997).

    Article  Google Scholar 

  31. M. Eggers, M. Hogan, R.K. Reich, J. Lamture, D. Ehrlich, M. Hollis, B. Kosicki, T. Powdrill, K. Beattie, S. Smith, R. Varma, R. Gangadharan, A. Mallik, B. Burke, and D. Wallace, Biotechniques 17, 516–524 (1994).

    Google Scholar 

  32. R.C. Anderson, G. McGall, and R.J. Lipshutz, Polynucleotide Arrays for Genetic Sequence Analysis, in Topics In Current Chemistry 194, 117, A. Manz, H. Becker, Ed. (Springer Verlag, Heidelberg, Germany, 1998).

    Google Scholar 

  33. G. Walraff, J. Labadie, P. Brock, R. DiPetro, T. Nguyen, T. Huynh, W. Hinsberg, and G.H. McGall, Chemtech 27, 22 (1997).

    Google Scholar 

  34. G. McGall, J. Labadie, P. Brock, G. Wallraff, G. Nguyen, and W. Hinsberg, Proc. Natl. Acad. Sci. (USA) 93, 13555 (1996).

    Article  Google Scholar 

  35. J.E. Beecher, G.H. McGall, and M.J. Goldberg, Polym. Mater. Sci. Eng. 76, 597 (1997).

    Google Scholar 

  36. J.E. Beecher, G.H. McGall, and M.J. Goldberg, Polym. Mater. Sci. Eng. 77, 394 (1997).

    Google Scholar 

  37. G.H. McGall, A.D. Barone, M. Diggelman, S.P.F.A. Fodor, E. Gentalen, and N. Ngo, J.A.C.S. 119, 5081 (1997).

    Article  Google Scholar 

  38. G. Yershov, V. Barsky, A. Belgovskiy, E. Kirillov, E. Kreindlin, I. Ivanov, S. Parinov, D. Gushin, A. Drobishev, S. Dubiley, and A. Mirzabekov, Proc. Nat. Acad. Sci (USA) 93, 4913 (1996).

    Article  Google Scholar 

  39. D. Proudnikov, E. Timofeev, and A. Mirzabekov, Anal. Biochem. 259, 34 (1998).

    Article  Google Scholar 

  40. A. Blanchard, in Genetic Engineering, Principles and Methods (Vol. 20) (J. Sedlow, ed.) 111–124 (Plenum Press, 1998).

  41. A.P. Blanchard, R.J. Kaiser, and L.E. Hood, Biosens. & Bioelectronics 11, 687 (1996).

    Article  Google Scholar 

  42. R.G. Sosnowski, G. Tu, W.F. Butler, J.P. O'Connell, and M.J. Heller, Proc. Natl. Acad. Sci. (USA) 94, 1119 (1997).

    Article  Google Scholar 

  43. O'Donnell-Maloney, C.L. Smith, and C.R. Cantor, Trends in Biotechnology 14, 401 (1996).

    Article  Google Scholar 

  44. E.M. Southern, Trends in Genetics 12, 110 (1996).

    Article  Google Scholar 

  45. T.J. Meade and J.F. Kayyem, Angew. Chem. Int. Ed. Engl. 34, 352 (1995).

    Article  Google Scholar 

  46. S.O. Kelley, R.E. Holmin, E.D.A. Stemp, and J.K. Barton, J. Am. Chem. Soc. 119, 9861 (1997).

    Article  Google Scholar 

  47. F.D. Lewis, T. Wu, Y. Zhang, R.L. Letsinger, S.R. Greenfield, and M.R. Wasielewski, Science 277, 673 (1998).

    Article  Google Scholar 

  48. J Kayyem, as quoted in R.M. Service, Science 282, 396 (1998).

    Article  Google Scholar 

  49. J. Baker-Jarvis. To be published.

  50. H.H. Thorp, Trends in Biotechnology 16, 117 (1998).

    Article  Google Scholar 

  51. K.L. Michael, L.C. Taylor, A.L. Schultz, and D.R. Walt, Anal. Chem. 70, 1242 (1998).

    Article  Google Scholar 

  52. R.J. Fulton, R.L. McDade, P.L. Smith, L.J. Kienker, and J.R. Kettman Jr, Clinical Chemistry 43, 1749 (1997).

    Google Scholar 

  53. W. Mandecki, Electronically-Indexed Solid-Phase Assay for Biomolecules, U.S. Patent 5,641,634. Issued June 24, 1997.

  54. J.A. Monforte and C.H. Becker, Nature Medicine 3, 158 (1997).

    Article  Google Scholar 

  55. L.M. Smith, Nature Biotechnology 14, 1084 (1996).

    Article  Google Scholar 

  56. D.P. Little, T.J. Cornish, M.J. O'Donnell, A. Braun, R.J. Cotter, and Koster, Anal. Chem. 69, 4540 (1997).

    Article  Google Scholar 

  57. M.J. O'Donnell, K. Tang, H. Koster, C.L. Smith, and C.R. Cantor, Anal. Chem. 69, 2438 (1997).

    Article  Google Scholar 

  58. G.C. Hilton, J.M. Martinis, D.A. Wollman, K.D. Irwin, I.L. Dulcie, D. Gerber, P.M. Gillevet, and D. Twerenbold, Nature 391, 672 (1998).

    Article  Google Scholar 

  59. J. Cheng, L.J. Kricka, E.L. Sheldon, and P. Wilding, Sample Preparation in Microstructure Devices, in Topics in Current Chemistry 194, A. Manz, H. Becker, Ed. (Springer-Verlag, Heidelberg, Germany, 1998).

    Google Scholar 

  60. P. Wilding, L.J. Kricka, J. Cheng, G. Hvichia, M.A. Shoffner, and P. Fortina, Anal. Biochem. 257, 95 (1998).

    Article  Google Scholar 

  61. J. Cheng, E.L. Sheldon, L. Wu, M.J. Heller, and J.P. O'Connell, Anal. Chem. 70, 2321 (1998).

    Article  Google Scholar 

  62. J. Cheng, E.L. Sheldon, L. Wu, A. Uribe, L.O. Gerrue, J. Carrino, M.J. Heller, and J.P. O'Connell, Nature Biotechnology 16, 541 (1998).

    Article  Google Scholar 

  63. M.A. Burns, B.N. Johnson, S.N. Brahmasandra, K. Handique, J.R. Webster, M. Krishnan, T.S. Sammarco, P.M. Man, D. Jones, D. Heldsinger, C.H. Mastrangelo, and D.T. Burke, Science 282, 484 (1998).

    Article  Google Scholar 

  64. R.C. Anderson, G.J. Bogdan, Z. Barniv, T.D. Dawes, J. Winkler, and K. Roy, Transducers 97, 477 (1997).

    Google Scholar 

  65. R.C. Anderson, G.J. Bogdan, A. Puski, and X. Su, Genetic Analysis Systems: Improvements and Methods Solid State Sensor and Actuator Workshop, Hilton Head, SC, June 8–11, 1998, 7–10.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abramowitz, S. DNA Analysis in Microfabricated Formats. Biomedical Microdevices 1, 107–112 (1999). https://doi.org/10.1023/A:1009992306940

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1009992306940

Keywords

Navigation