Abstract
The use of microfabricated DNA analysis tools utilizing microfluidics will provide the next generation of inexpensive DNA diagnostics. It will also provide methodologies to measure gene expression in a massively parallel manner, eventually providing the methodologies to measure most or all the human genes of significance on a single chip. These technologies, including PCR analysis, electrophoresis and gene chips are described using examples from the archival literature.
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References
S. Abramowitz, Trends in Biotechnology 14, 397 (1996).
F.S. Collins, A. Patrinos, E. Jordan, A. Chakravarti, R. Gesteland, and L. Waters, Science 282, 682 (1998).
A listing of all sequenced genomes and references can be found in the TIGR home page: www.tigr.org.
R. Waterston and J.E. Sulston, Science 282, 53 (1998).
J.C. Venter, M.D. Adams, G.G. Sutton, A.R. Kerlavage, H.O. Smith, and M. Hunkapiller, Science 280, 1540 (1998).
F.C. Collins, M.S. Guyer, and A. Chakravarti, Science 278, 1580 (1998).
C.A. Dahl and R.L. Strausberg, SPIE 190, 3680 (1996).
The use of these technologies in the analysis of cancer genomics may be found on the National Cancer Institute Cancer Genome Anatomy Project website: www.nlm.nih.gov/ncicgap.
K.E. Petersen, W.A. McMillan, T.A. Kovacs, M.A. Northup, and L.A. Christel, Biomedical Devices 1, 71 (1998).
A. Van den Berg and T.S.J. Lammerink, Micro Total Analysis Systems: Microfluidic Aspects, Integration Concept and Applications in Topics in Current Chemistry 194, 21, A. Manz, H. Becker, Ed. (Springer-Verlag, Heidelberg, Germany, 1998).
D. Qin, Y. Xia, J.A. Rogers, R.J. Jackman, X-M. Zhao, and G.M. Whitesides, Microfabrication, Microstructure and Microsystems, in Current Chemistry 194, 117, A. Manz, H. Becker, Ed. (Springer Verlag Heidelberg, Germany, 1998), 1.
M.S. Ibrahim, R.S. Loftis, P.B. Jahrling, E.A. Henschal, V.W. Weedn, M.A. Northrup, and P. Belgrader, Anal. Chem. 70, 2013 (1998).
M.A. Northrup, B. Bennet, D. Hadley, P. Landre, S. Lehew, J. Richards, and P. Stratton, Anal. Chem. 70, 918 (1998).
M.A. Northrup, B. Beeman, B. Benett, D. Hadley, P. Landre, and Lehew, A Miniature Integrated Nucleic Acid Analysis System, in Automation Technologies for Genome Characterization T.J. Beugelsdijk, Ed. (John Wiley & Sons, New York, 1997).
P. Belgrader, J.K. Smith, V.W. Weedn, and M.A. Northrup, J. Forensic Sc. 43, 315 (1998).
S. Harvey, M. Albin, L. Lebak, I. Mowatt, T. Schuerlin, and E. Principe, Biomedical Devices 1, 65 (1998).
T.M. Woudenberg, E.S. Winn-Dean, and M. Albin, High Density PCR and Beyond, Analytical Methods and Instrumentation, Special Issue µTAS '96 55 (1996).
T.B. Taylor, E.S. Winn-Deen, E. Picozza, T.M. Woudenberg, and M. Albin, Nucleic Research 25, 3164 (1997).
J. Cheng, L.C. Waters, P. Fortina, G. Hvichia, S.C. Jacobson, J.M. Ramsey, L.J. Kricka, and P. Wilding, Anal. Biochem. 257, 101 (1998).
M.U. Kopp, A.J. deMello, and A. Manz, Science 280, 1046 (1998).
P.C. Simpson, A.T. Wooley, and R.A. Mathies, Biomedical Devices 1, 7 (1998).
C.S. Effenhauser, Integrated Chip-Based Microcolumn Separation Systems in Topics in Current Chemistry 194, 51, A. Manz, H. Becker, Ed. (Springer-Verlag, Heidelberg, Germany, 1998).
A.T. Wooley and R.A. Mathies, Anal. Chem. 67, 3676 (1995).
R.M. McCormick and R.J. Nelson, M.G. Alonso-Amigo, D.J. Benvegnu, and H.H. Hooper, Anal. Chem. 69, 2626 (1997).
A.T. Wooley, D. Hadley, P. Landre, A. deMello, and R.A. Mathies, Anal. Chem. 68, 4081 (1996).
P.C. Simpson, D. Roach, A.T. Wooley, T. Thorsen, R. Johnston, G.F. Sensabaugh, and R.A. Mathies, Proc. Natl. Acad. Sci. (USA) 95, 2256 (1998).
C.H. Li and D.J. Harrison, Anal. Chem. 69, 1564 (1997).
S.C. Jacobson and J.M. Ramsey, Anal. Chem. 68, 720 (1996).
M. Schena, R.A. Heller, T.P. Theriault, K. Konrad, E. Lachenmeier, and R.W. Davis, Trends in Biotechnology 16, 301 (1998).
R.A. Heller, M. Schena, A. Chai, D. Shalon, T. Bedilion, J. Gilmore, D.E. Wooley, and R.W. Davis, Proc. Nat. Acad. Sci., (USA) 94, 2150 (1997).
M. Eggers, M. Hogan, R.K. Reich, J. Lamture, D. Ehrlich, M. Hollis, B. Kosicki, T. Powdrill, K. Beattie, S. Smith, R. Varma, R. Gangadharan, A. Mallik, B. Burke, and D. Wallace, Biotechniques 17, 516–524 (1994).
R.C. Anderson, G. McGall, and R.J. Lipshutz, Polynucleotide Arrays for Genetic Sequence Analysis, in Topics In Current Chemistry 194, 117, A. Manz, H. Becker, Ed. (Springer Verlag, Heidelberg, Germany, 1998).
G. Walraff, J. Labadie, P. Brock, R. DiPetro, T. Nguyen, T. Huynh, W. Hinsberg, and G.H. McGall, Chemtech 27, 22 (1997).
G. McGall, J. Labadie, P. Brock, G. Wallraff, G. Nguyen, and W. Hinsberg, Proc. Natl. Acad. Sci. (USA) 93, 13555 (1996).
J.E. Beecher, G.H. McGall, and M.J. Goldberg, Polym. Mater. Sci. Eng. 76, 597 (1997).
J.E. Beecher, G.H. McGall, and M.J. Goldberg, Polym. Mater. Sci. Eng. 77, 394 (1997).
G.H. McGall, A.D. Barone, M. Diggelman, S.P.F.A. Fodor, E. Gentalen, and N. Ngo, J.A.C.S. 119, 5081 (1997).
G. Yershov, V. Barsky, A. Belgovskiy, E. Kirillov, E. Kreindlin, I. Ivanov, S. Parinov, D. Gushin, A. Drobishev, S. Dubiley, and A. Mirzabekov, Proc. Nat. Acad. Sci (USA) 93, 4913 (1996).
D. Proudnikov, E. Timofeev, and A. Mirzabekov, Anal. Biochem. 259, 34 (1998).
A. Blanchard, in Genetic Engineering, Principles and Methods (Vol. 20) (J. Sedlow, ed.) 111–124 (Plenum Press, 1998).
A.P. Blanchard, R.J. Kaiser, and L.E. Hood, Biosens. & Bioelectronics 11, 687 (1996).
R.G. Sosnowski, G. Tu, W.F. Butler, J.P. O'Connell, and M.J. Heller, Proc. Natl. Acad. Sci. (USA) 94, 1119 (1997).
O'Donnell-Maloney, C.L. Smith, and C.R. Cantor, Trends in Biotechnology 14, 401 (1996).
E.M. Southern, Trends in Genetics 12, 110 (1996).
T.J. Meade and J.F. Kayyem, Angew. Chem. Int. Ed. Engl. 34, 352 (1995).
S.O. Kelley, R.E. Holmin, E.D.A. Stemp, and J.K. Barton, J. Am. Chem. Soc. 119, 9861 (1997).
F.D. Lewis, T. Wu, Y. Zhang, R.L. Letsinger, S.R. Greenfield, and M.R. Wasielewski, Science 277, 673 (1998).
J Kayyem, as quoted in R.M. Service, Science 282, 396 (1998).
J. Baker-Jarvis. To be published.
H.H. Thorp, Trends in Biotechnology 16, 117 (1998).
K.L. Michael, L.C. Taylor, A.L. Schultz, and D.R. Walt, Anal. Chem. 70, 1242 (1998).
R.J. Fulton, R.L. McDade, P.L. Smith, L.J. Kienker, and J.R. Kettman Jr, Clinical Chemistry 43, 1749 (1997).
W. Mandecki, Electronically-Indexed Solid-Phase Assay for Biomolecules, U.S. Patent 5,641,634. Issued June 24, 1997.
J.A. Monforte and C.H. Becker, Nature Medicine 3, 158 (1997).
L.M. Smith, Nature Biotechnology 14, 1084 (1996).
D.P. Little, T.J. Cornish, M.J. O'Donnell, A. Braun, R.J. Cotter, and Koster, Anal. Chem. 69, 4540 (1997).
M.J. O'Donnell, K. Tang, H. Koster, C.L. Smith, and C.R. Cantor, Anal. Chem. 69, 2438 (1997).
G.C. Hilton, J.M. Martinis, D.A. Wollman, K.D. Irwin, I.L. Dulcie, D. Gerber, P.M. Gillevet, and D. Twerenbold, Nature 391, 672 (1998).
J. Cheng, L.J. Kricka, E.L. Sheldon, and P. Wilding, Sample Preparation in Microstructure Devices, in Topics in Current Chemistry 194, A. Manz, H. Becker, Ed. (Springer-Verlag, Heidelberg, Germany, 1998).
P. Wilding, L.J. Kricka, J. Cheng, G. Hvichia, M.A. Shoffner, and P. Fortina, Anal. Biochem. 257, 95 (1998).
J. Cheng, E.L. Sheldon, L. Wu, M.J. Heller, and J.P. O'Connell, Anal. Chem. 70, 2321 (1998).
J. Cheng, E.L. Sheldon, L. Wu, A. Uribe, L.O. Gerrue, J. Carrino, M.J. Heller, and J.P. O'Connell, Nature Biotechnology 16, 541 (1998).
M.A. Burns, B.N. Johnson, S.N. Brahmasandra, K. Handique, J.R. Webster, M. Krishnan, T.S. Sammarco, P.M. Man, D. Jones, D. Heldsinger, C.H. Mastrangelo, and D.T. Burke, Science 282, 484 (1998).
R.C. Anderson, G.J. Bogdan, Z. Barniv, T.D. Dawes, J. Winkler, and K. Roy, Transducers 97, 477 (1997).
R.C. Anderson, G.J. Bogdan, A. Puski, and X. Su, Genetic Analysis Systems: Improvements and Methods Solid State Sensor and Actuator Workshop, Hilton Head, SC, June 8–11, 1998, 7–10.
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Abramowitz, S. DNA Analysis in Microfabricated Formats. Biomedical Microdevices 1, 107–112 (1999). https://doi.org/10.1023/A:1009992306940
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DOI: https://doi.org/10.1023/A:1009992306940