Abstract
This article describes the design, fabrication and initial testing of an enzymatic microreactor using Si, glass, epoxy and Perspex components. The microreactor geometry was defined by a photostructured EPON SU-8 spacer. This gave two chambers, an upstream chamber (vol. ∼2.7 μl) which was packed with control pore glass (CPG) and a downstream chamber (vol. ∼1.5 μl) which contained the detectors. Eleven EPON SU-8 columns separated the chambers and prevented the CPG from passing. Glucose detection via glucose oxidase immobilized to the CPG was used as a model system. Conversion efficiencies between 15 and 60% at flow rates of 150 and 26 μ1 min−1 respectively were obtained. Overall the system was found to be robust and to have a lifetime of at least one month.
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References
J.M.S. Cabral and J.F. Kennedy, in Protein Immobilization, Fundamentals and Applications edited by R.F. Taylor (Marcel Dekker, Inc, New York, 1991).
J. Drott, K. Lindstrom, L. Rosengren, and T. Laurell, J. Micromech Microeng 7, 14 (1997).
T. Laurell and L. Rosengren, Sensors and Actuators B 18–19, 614 (1994).
T. Laurell, J. Drott, and L. Rosengren, Biosensors & Bioelectronics 10, 289 (1995).
K.Y. Lee, N. LaBianca, S.A. Riston, S. Zolgharnain, J.D. Gelorme, J. Shaw, and T.H.-P-Chang, J. Vac. Sci. Technol. B 13, 3012 (1995).
H. Lorenz, M. Despont, N. Fahrni, N. LaBianca, P. Renaud, and P. Vettiger, J. Micromech. Microeng. 7, 121 (1997).
D.J. Strike, Ph.E. Michel, E. L'Hostis, G.C. Fiaccabrino, N.F. de Rooij, and M. Koudelka-Hep, Digest of Technical Papers of the 10th International Conference on Solid-state Sensors and Actuators, Transducers'99, Sendai, Japan, June 7–10, 1999, 1, p. 130.
G.C. Fiaccabrino and M. Koudelka-Hep, Electroanalysis 10, 217 (1998).
Y. Murakami, T. Takauchi, K. Yokoyama, E. Tamiya, I. Karube, and M. Suda, Anal. Chem. 65, 2731 (1993).
P. Renaud, H. Van Lintel, M. Heuschkel, and L. Guérin, in Micro Total Analysis Systems '98, edited by D.J. Harrison and A. van den Berg (Kluwer Academic Publishers, London, 1998), p. 17.
D.J. Strike, P. Thébaud, Ph. Arquint, M. Koudelka-Hep, N.F. de Rooij, and M.G.H. Meijerink, in Microsystem Technology for Chemical and Biological Microreactors (DECHEMA monographs, vol. 132) (VCH, New York, 1996), p. 48.
P. Woias, M. Richter, E. Yacoub-George, H. Wolf, and T. Abel, Anal Methods & Instrumentation, Special Issue μTAS '96, 256 (1996).
B. Xie, B. Danielsson, P. Norberg, F. Winquist, and I. Lundstrom, Sensors and Actuators B 6, 127 (1992).
B. Xie, B. Danielsson, and F. Winquist, Sensors and Actuators B 15–16, 443 (1993).
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Strike, D., Fiaccabrino, GC., Koudelka-Hep, M. et al. Enzymatic Microreactor Using Si, Glass and EPON SU-8. Biomedical Microdevices 2, 175–178 (2000). https://doi.org/10.1023/A:1009972210811
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DOI: https://doi.org/10.1023/A:1009972210811