Abstract
The recognition of many enzymes as nanoscale molecular motors has opened the door for the potential creation of hybrid organic/inorganic nano-electro-mechanical (NEMS) devices. The long-term objective of this research is the integration of F 1-ATPase with NEMS to produce useful nanoscale devices. A thermostable F 1-ATPase coding sequence has been isolated, cloned, and engineered for high-level protein expression. Precise positioning, orientation, and spacing of individual F 1-ATPase molecules were achieved on patterned nickel arrays produced using electron beam lithography. An efficient and accurate assay was developed to evaluate the performance of individual F 1-ATPase motors, and confirmed a three-step mechanism of γ subunit rotation during ATP hydrolysis. Further assessment of the biophysical and bioengineering properties of FF 1-ATPase currently are being conducted, as well as the construction of a hybrid NEMS device powered by FF 1-ATPase. The evolution of this technology will permit the creation of novel classes of nanoscale, hybrid devices.
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Bachand, G.D., Montemagno, C.D. Constructing Organic/Inorganic NEMS Devices Powered by Biomolecular Motors. Biomedical Microdevices 2, 179–184 (2000). https://doi.org/10.1023/A:1009924327649
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DOI: https://doi.org/10.1023/A:1009924327649