Abstract
Optical and fluidics systems have been developed as central components for an automated array biosensor. Disposable planar waveguides are patterned with immobilized capture antibodies using a physically isolated patterning (PIP) method. The PIP method enables simultaneous deposition of several antibodies and completely circumvents cross-immobilization problems encountered with other array deposition processes. A multi-channel fluidics cell allows numerous assays to be performed on the patterned waveguide. The sensing arrays are optically interrogated using a diode laser with a tailored output to optimize coupling to and maximize excitation uniformity within the waveguide. A patterned cladding is employed to optically isolate the waveguide from perturbations induced by the permanently attached flow cells. Compact optics image the evanescently excited fluorescence onto a large area, cooled CCD array. The image data is processed and automated signal analysis corrects for local background and noise variations.
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References
G.P. Anderson, J.P. Golden, and F.S. Ligler, Biosens. & Bioelectron. 8, 249 (1993).
G.P. Anderson and J.P. Golden, US patent 5,430,813 (1995).
D. Axelrod, T.P. Burghardt, N.L. Thompson, Ann. Rev. Biophys. Bioeng. 13, 247 (1984).
S.K. Bhatia, F.C. Shriver-Lake, K.J. Prior, J.H. Georger, J.M. Calvert, R. Bredehorst, and F.S. Ligler, Anal. Biochem. 178, 408 (1989).
A.S. Blawas, T.F. Oliver, M.C. Pirrung, and W.M. Reichert, Langmuir 14(15), 4243 (1998).
D.W. Conrad and P.T. Charles, US Patent 5,736,257 (1998).
D.W. Conrad, A.V. Davis, S.K. Golightley, J.C. Bart, and F.S. Ligler, SPIE 2978,12 (1997).
E. Delamarche, A. Bernard, H. Schmid, B. Michel, and H. Biebuyck, Science 276, 779 (1997).
J. Dübendorfer and R.E. Kunz, Appl. Optics. 37, 1890 (1998).
G.L. Duveneck, M. Ehrat, D. Neuschäfer, B. Danielzik, M. Heming, and J. Segner, WO 9533197 (1995).
G.L. Duveneck, M. Pawlak, D. Neuschäfer, E. Bär, W. Budach, U. Pieles, and M. Ehrat, Sensors & Actuators B 38–39, 88 (1997).
S.P.A. Fodor, J.L. Read, M.C. Pirrung, L. Stryer, A.T. Lu, and D. Solas, Science 251, 767 (1991).
J.P. Golden, US patent 5,827,748 (1998).
J.N. Herron, D.A. Christensen, K.D. Caldwell, V. Janatová, S.-C. Huang, and H.-K. Wang, US patent 5,512,492 (1996).
J.N. Herron, D.A. Christensen, H.-K. Wang, K.D. Caldwell, V. Janatová, and S.-C. Huang, US patent 5,677,196 (1997).
R. Kapur, B.J. Spango, M-S. Chen, J.M. Calvert, and A.S. Rudolph, J. Biomed. Materials Research 33, 205 (1996).
A. Katerkamp, WO 9802732 (1997).
E. Kim, Y. Xia, G.M. Whitesides, Adv. Mat. 8(3), p. 245 (1996).
K.D. King, G.P. Anderson, K.E. Bullock, M.J. Regina, E.W. Saaski, and F.S. Ligler, Biosens. Bioelectron. 14(2), 163 (1999).
S. Leslie, N. Shah, T. Adams, A.S. Rudolph, R. Kapur, Biotechnology and Bioengineering (submitted 1998).
F.S. Ligler, D.W. Conrad, J.P. Golden, M.J. Feldstein, B.D. MacCraith, S.D. Balderson, J. Czarnaski, and C.A. Rowe, Proc. SPIE 3258, 50 (1998).
W.F. Love and L.J. Button, SPIE 990, 175 (1998).
B.D. Martin, B.P. Gaber, C.H. Patterson, and D.C. Turner, Langmuir 14(15), 3971 (1998).
N.J. Mourlas, D. Jaeggi, A.F. Flannery, B.L. Gray, B.P. van Drieënhuizen, C.W. Storment, N.I. Maluf, and G.T.A. Kovacs, Micro Total Analysis Systems '98 (Kluwer Academic Publishers, The Netherlands, 1998) 27.
C.A. Rowe, S.B. Scruggs, M.J. Feldstein, J.P. Golden, and F.S. Ligler, Anal. Chem. 71(2), 433 (1999a).
C.A. Rowe, M.J. Feldstein, J.P. Golden, S.B. Scruggs, B. MacCraith, L.M. Tender, and F.S. Ligler, Anal. Chem. (submitted 1999b).
R.M. Wadkins, J.P. Golden, L.M. Pritsiolas, and F.S. Ligler, Biosens. & Bioelectron. 13(3–4), 407 (1998).
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Feldstein, M.J., Golden, J.P., Rowe, C.A. et al. Array Biosensor: Optical and Fluidics Systems. Biomedical Microdevices 1, 139–153 (1999). https://doi.org/10.1023/A:1009900608757
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DOI: https://doi.org/10.1023/A:1009900608757