Abstract
Methods are described to establish distinct cell cultures from bovine placental cotyledon. The villous tissue of the bovine placental cotyledon is collected and dissociated with 0.125% trypsin. The cells are then cultured in three different media: a serum-free medium, a growth factor supplemented medium, and a medium with 10% serum. A polygonal cell line grew out of the serum-containing medium, a fan-shaped cell line grew out of the serum-free medium, and an epitheloid cell line grew out of the growth factor supplemented medium. These cells maintained their morphology when grown in serum containing medium. The preference of distinct cells for different media in vitro reflects the in vivo physiological regulation of these cells. These distinct cultures re ideal to study the extrinsic and interactive factorsin bovine placenta.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cooper MS, Schliwa M (1986). Motility of cultured fish epidermal cells in the presence and absence of direct current electric fields. J Cell Biol 102:1384-1399.
Euteneuer U, Schliwa M (1984). Persistent, directional motility of cells and cytoplasmic fragments in the absence of microtubules. Nature 310: 58-61.
Kliman HJ, Nestler JE, Sermasi E, Singer JM, Strauss III JF (1986). Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinol 118: 1567-1582.
Lee J, Ishihara A, Theriot JA, Jacobson K. Princples of locomotion for simple-shaped cells. Nature 362: 167-171.
Loke YW (1983). Human trophoblast in culture. In: Loke YW, Whyte A (eds), Biology of Trophoblast, pp 663-667. Amsterdam: Elsevier Sciences Publishers.
Low BG, Hansen PJ, Drost M (1991). Stimulation of proliferation of bovine placental cells by products of activated mononuclear leukocytes. In Vitro Cellular and Development Biology 27A: 639-645.
Matamoros RA, Caamano L, Lamb SV, Reimers TJ (1994). Estrogen production by bovine binucleate and mononucleate trophoblastic cells in vitro. Biol Reprod 51: 486-492.
Munson L, Chandler SK, Schlafer DH (1988). Longterm culture of bovine trophoblastic cells. J Tiss Cult Meth 11: 123-128.
Murphy TL, Sakamoto T, Hinton DR, Spee C, Gundimeda U, Soriano D, Gopalakrishna R, Ryan SJ (1995). Migration of retinal pigment epithelium cells in vitro is regulated by protein kinase C. Exp Eye Res 60: 683-695.
Reimers TJ, Ullmann MB, Hansel W (1985). Progesterone and prostanoid production by bovine binucleate trophoblastic cells. Biol Reprod 33: 1227-1236.
Rexroad CE, Casida Le, Tyler WJ (1974). Crownrump length of fetuses in purebred Holstein-Friesian cows. J Dairy Sci 57: 346-347.
Shemesh M, Harel-Markowitz E, Gurevich M, Shore LS (1994). Staurosporine stimulates progesterone production by bovine placental cells. Biol Reprod 51: 146-151.
Rights and permissions
About this article
Cite this article
Feng, S., Peter, A.T. & Asem, E. Establishment of a pure culture of distinct cell types from bovine placental cotyledon. Methods Cell Sci 22, 101–106 (2000). https://doi.org/10.1023/A:1009822117191
Issue Date:
DOI: https://doi.org/10.1023/A:1009822117191