Advertisement

Molecular Breeding

, Volume 5, Issue 2, pp 167–175 | Cite as

Identification of molecular markers for the detection of the yellow rust resistance gene Yr17 in wheat

  • Olivier Robert
  • Christine Abelard
  • Françoise Dedryver
Article

Abstract

The Yr17 gene, which is present in many European wheat cultivars, displays yellow rust resistance at the seedling stage. The gene introduced into chromosome 2A from Aegilops ventricosa was previously found to be closely linked (0.5 cM) to leaf and stem rust resistance genes Lr37 and Sr38, respectively. The objective of this study was to identify molecular markers linked to the Yr17 gene. We screened with RAPD primers, for polymorphism, the DNAs of cv. Thatcher and the leaf rust-resistant near-isogenic line (NIL) RL 6081 of cv. Thatcher carrying the Lr37 gene. Using a F2 progeny of the cross between VPM1 (resistant) and Thésée (susceptible), the RAPD marker OP-Y15580 was found to be closely linked to the Yr17 gene. We converted the OP- Y15580 RAPD marker into a sequence characterized amplified region (SCAR). This SCAR marker (SC-Y15) was linked at 0.8 ± 0.7 cM to the Yr17 resistance gene. We tested the SC-Y15 marker over a survey of 37 wheat cultivars in order to verify its consistency in different genetic backgrounds and to explain the resistance of some cultivars against yellow rust. Moreover, we showed that the Xpsr150-2Mv locus marker of Lr gene described by Bonhomme et al. [6] which possesses A. ventricosa introgression on the 2A chromosome was also closely linked to the Yr17 gene. Both the SCAR SC-Y15 and Xpsr150-2Mv markers should be used in breeding programmes in order to detect the cluster of the three genes Yr17, Lr37 and Sr38 in cross progenies.

wheat DNA markers yellow rust resistance Yr17 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Autrique E, Singh RP, Tanksley SD, Sorrells ME: Molecular markers for four leaf rust resistance genes introgressed into wheat from wild relatives. Genome 38: 75–83 (1995).PubMedGoogle Scholar
  2. 2.
    Bariana HS, McIntosh RA: Cytogenetic studies in wheat XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome 36: 476–482 (1993).PubMedGoogle Scholar
  3. 3.
    Bariana HS, McIntosh RA: Characterisation and origin of rust and powdery mildew resistance genes in VPM1 wheat. Euphytica 76: 53–61 (1994).CrossRefGoogle Scholar
  4. 4.
    Barret P, Delourme R, Renard M, Domergue F, Lessire R, Delseny M, Roscoe TJ: The rapeseed FAEI gene is linked to the E1 locus associated with variation in the content of erucic acid. Theor Appl Genet 96: 177–186 (1991).CrossRefGoogle Scholar
  5. 5.
    Bayles RA, Stigwood PL: Yellow rust of wheat. U.K. Cereal Pathogen Virulence Survey Annual Report, pp. 13–17 (1995).Google Scholar
  6. 6.
    Bonhomme A, Gale MD, Koebner RMD, Nicolas P, Jahier J, Bernard M: RFLP analysis of an Aegilops ventricosa chromosome that carries a gene conferring resistance to leaf rust (Puccinia recondita) when transferred to hexaploid wheat. Theor Appl Genet 90: 1042–1048 (1995).CrossRefGoogle Scholar
  7. 7.
    Dedryver F, Jubier MF, Thouvenin J, Goyeau H: Molecular markers linked to the leaf rust resistance gene Lr24 in different wheat cultivars. Genome 39: 830–835 (1996).PubMedGoogle Scholar
  8. 8.
    Feuillet C, Messmer M, Schachermayr G, Keller B: Genetic and physical characterisation of the Lr1 leaf rust resistance locus in wheat (Triticum aestivum L.). Mol Gen Genet 248: 553–562 (1995).PubMedCrossRefGoogle Scholar
  9. 9.
    Gerechter-Amitai ZK, van Silfhout CH, Grama A, Kleitman F: Yr15, a new gene for resistance to Puccinia striiformis in Triticum dicoccoides sel G-25. Euphytica 43: 187–190 (1989).CrossRefGoogle Scholar
  10. 10.
    Jones CJ, Edwards KJ, Castaglione S, Winfield MO, Sala F, van de Wiel C, Bredemeijer G, Vosman B, Matthes M, Daly A, Brettschneider R, Bettini P, Buiatti M, Maestri E, Malcevschi A, Marmiroli N, Aert R, Volckaert G Rueda J, Linacero R Vazquez A, Karp A: Reproducibility testing of RAPD, AFLP, and SSR markers in plants by network of European laboratories. Mol Breed 3: 381–390 (1997).CrossRefGoogle Scholar
  11. 11.
    Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L: MAPMAKER: an interactive computer package for constructing primary genetic maps of experimental and natural populations. Genomics 1: 174–181 (1987).PubMedCrossRefGoogle Scholar
  12. 12.
    Line RF, Chen XM, Gale MD, Leung H: Development of molecular markers associated with quantitative trait loci in wheat for durable resistance to Puccinia striiformis. In: Proceedings of the 9th European and Mediterranean Cereal Rust and Powdery Mildews Conference p. 234 (1996).Google Scholar
  13. 13.
    Maia N: Obtention de blés tendres résistants au piétin-verse par croisement interspécifiques blés × Aegilops. C R Acad Agric Fr, pp. 149–154 (1967).Google Scholar
  14. 14.
    Maniatis T, Fritsch EF, Sambrook L: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).Google Scholar
  15. 15.
    McMillin DE, Johnson JW, Roberts JJ: Linkage between endopeptidase Ep-D1d and a gene conferring leaf rust resistance (Lr19) in wheat. Crop Sci 33: 1201–1203 (1993).CrossRefGoogle Scholar
  16. 16.
    McNeal FH, Konzak CF, Smith EP, Tate WS, Russel TS: A uniform system for recording and processing cereal research data. US Agric Res Serv 42: 34–121 (1971).Google Scholar
  17. 17.
    Paran I, Michelmore RW: Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85: 985–993 (1993).CrossRefGoogle Scholar
  18. 18.
    Pearson WR, Lipman DJ: Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85: 2444–2448 (1988).PubMedCrossRefGoogle Scholar
  19. 19.
    Procurier JD, Townley-Smith TF, Prashar S, Gray M, Kim WK, Czamecki E, Dyck PL: PCR-based RAPD/DGGE markers linked to leaf rust resistance genes Lr29 and Lr25 in wheat (Triticum aestivum L.). J Genet Breed 49: 87–92 (1995).Google Scholar
  20. 20.
    Ribault JM, Hu X, Hoisington D, Gonzalez de Leon D: Use of STSs and SSRs as rapid and reliable preselection tools in marker-assisted selection-backcross scheme. Plant Mol Biol Rep 15: 154–162 (1997).Google Scholar
  21. 21.
    Riley R, Chapman V, Johnson R: Introduction yellow rust resistance of Aegilops comosa into wheat by genetically induced homoeologous recombination. Nature 217: 383–384 (1968).CrossRefGoogle Scholar
  22. 22.
    Ritter E, Gebhart C, Salamini F: Estimation of recombinaison frequencies and construction of RFLP linkage maps in plants from crosses between heterozygous parents. Genetics 125: 645–654 (1990).PubMedGoogle Scholar
  23. 23.
    Rogers SO, Bendich AJ: Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5: 69–76 (1985).CrossRefGoogle Scholar
  24. 24.
    Sambrook J, Frisch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).Google Scholar
  25. 25.
    Schachermayr G, Siedler H, Gale MD, Winzeler H, Winzeler M, Keller B: Identification and localization ofmolecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor Appl Genet 88: 110–115 (1994).CrossRefGoogle Scholar
  26. 26.
    Schachermayr GM, Messmer MM, Feuillet C, Winzeler H, Winzeler M, Keller B: Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat. Theor Appl Genet 90: 982–990 (1995).CrossRefGoogle Scholar
  27. 27.
    Schachermayr G, Feuillet C, Keller B: Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds. Mol Breed 3: 65–74 (1997).CrossRefGoogle Scholar
  28. 28.
    Sun GL, Fahima T, Korol AB, Turpeinen T, Grama A, Ronin YI, Nevo E: Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum diccoides. Theor Appl Genet 95: 622–628 (1997).CrossRefGoogle Scholar
  29. 29.
    Williams GK, Kubelik AR, Kenneth JL, Rafaski A, Scott VT: DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531–6535 (1990).PubMedGoogle Scholar
  30. 30.
    Zeller FJ: 1B / 1R wheat-rye chromosome substitutions and translocations. In: Sears ER, Sears LMS (eds) Proceedings of the 4th International Wheat Genetics Symposium, pp. 209–221 (1973).Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Olivier Robert
    • 1
  • Christine Abelard
    • 1
  • Françoise Dedryver
    • 1
  1. 1.INRA, Station d'Amélioration des PlantesLaboratoire CéréalesLe Rheu CédexFrance

Personalised recommendations