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Anti-Fas antibody-induced apoptosis in human colorectal carcinoma cell lines: role of the p53 gene

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Abstract

The cell surface Fas antigen transducts an apoptotic signal by its crosslinking with Fas ligand or anti-Fas antibody in a variety of human cultured cells. In this study, we examined the expression of Fas antigen and its mediation of apoptosis in six human colorectal carcinoma cell lines. A flow cytometric analysis revealed that LoVo, DLD-1, WiDr and SW837 cell lines showed higher expression levels of Fas antigen, in contrast to lower expression in COLO201 and COLO320DM. Interferon-γ enhanced the expression of Fas antigen in all of the cell lines examined. Both Fas ligand and Fas-associated phosphatase-1 (FAP-1) were expressed only in COLO320DM. Anti-Fas antibody induced apoptosis in LoVo carrying wild-type p53 gene, but not in the other five cell lines carrying mutated p53 gene. The transfection of wild-type p53 gene using an adenovirous vector upregulated P53 protein in WiDr and SW837 cells, both of which showed, however, no increase in apoptotic cells by anti-Fas antibody treatment. These results indicated that (1) Fas antigen was variably expressed, regardless of the p53 gene status and (2) the susceptibility to anti-Fas antibody-mediated apoptosis did not correlate to Fas, Fas ligand or FAP-1 expression levels. Therefore, we conclude that wild-type P53 expression might not necessarily be essential for Fas-mediated apoptosis in human colorectal carcinoma cell lines.

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Authors and Affiliations

  1. First Department of Pathology, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan

    H. Hayashi, S. Tatebe, M. Osaki, A. Goto, K. Sato & H. Ito

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  1. H. Hayashi
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  2. S. Tatebe
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  3. M. Osaki
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  4. A. Goto
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  5. K. Sato
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  6. H. Ito
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Hayashi, H., Tatebe, S., Osaki, M. et al. Anti-Fas antibody-induced apoptosis in human colorectal carcinoma cell lines: role of the p53 gene. Apoptosis 3, 431–437 (1998). https://doi.org/10.1023/A:1009662619907

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