This paper presents a new approach for plant improvement that interactively combines the use of DNA markers and conventional breeding. This approach involves selecting plants at early generation with a fixed, favorable genetic background at specific loci, conducting a single large-scale marker-assisted selection (SLS-MAS) while maintaining as much as possible the allelic segregation in the rest of the genome. First, the identification of elite lines presenting high allelic complementarity and being outstanding for traits of interest is required to capture favorable alleles from different parental lines. Second, after identification of the most favorable genomic regions for each selected parental line, those lines are intercrossed to develop segregating populations from which plants homozygous for favorable alleles at target loci are selected. One objective of the scheme is to conduct the marker-assisted selection only once, and it requires the selection of a minimum number of plants to maintain sufficient allelic variability at the unselected loci. Therefore, the selection pressure exerted on the segregating population is quite high and the screening of large populations is required to achieve the objectives of the scheme. No selection is applied outside the target genomic regions, to maintain as much as possible the Mendelian allelic segregation among the selected genotypes. After selection with DNA markers, the genetic diversity at un-selected loci may allow breeders to generate new varieties and hybrids through conventional breeding in response to various local needs. Although the single large-scale MAS scheme described here is oriented toward maize and large-scale breeding programs with substantial resources, the flexibility of this scheme would allow breeding programs to develop options compatible with local resources.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Austin DF, Lee M: Comparative mapping in F2:3 and F6:7 generations of quantitative traits loci for grain yield and yield components in maize. Theor Appl Genet 92: 817–826 (1996).
Beavis WD, Keim P: Identification of quantitative trait loci that are affected by environment. In: Kang MS, Gauch HG (eds) Genotype-by-Environment Interaction, pp. 123–149. CRC Press, Boca Raton, FL (1996).
Charcosset A, Essioux L: The effect of population structure on the relationship between heterosis and heterozygosity at marker loci. Theor Appl Genet 89: 336–343 (1994).
Cowen NM, Frey KJ: Relationship between genealogical distance and breeding behavior in oats (Avena sativa L.). Euphytica 36: 413–424 (1987).
Dudley JW: Modification of methods for identifying inbred lines useful for improving parents of elite single crosses. Crop Sci 27: 944–947 (1987).
Dudley JW: Molecular markers in plant improvement: manipulation of genes affecting quantitative traits. Crop Sci 33: 660–668 (1993).
Duvick DN: Genetic contribution to yield gains of U. S. hybrid maize, 1930 to 1980. In: Fehr WR (ed.) Genetic contributions to Yield Gains of Five Major Crop Plants, pp. 15–47. Special Publication 7, Crop Science Society of America, Madison, WI (1984).
Edwards MD, Page NJ: Evaluation of marker-assisted selection through computer simulation. Theor Appl Genet 88: 376–382 (1994).
Fodor SPA: Massively parallel genomics. Science 277: 393–395 (1997).
Gleeson AC: Spatial analysis. In: Kempton RA, Fox PN (eds.) Statistical Methods for Plant Variety Evaluation, pp. 68–85. Chapman and Hall, London (1997).
Gimelfarb A, Lande R: Simulation ofmarker assisted selection in hybrid populations. Genet Res Camb 63: 39–47 (1994).
Gu WK, Weeden NF, Yu J, Wallace DH: Large-scale, costeffective screening of PCR products in marker-assisted selection applications. Theor Appl Genet 91: 465–470 (1995).
Hallauer AR, Miranda JB: Quantitative Genetics in Maize Breeding. Iowa State University Press, Ames, IA (1981).
Hospital F, Charcosset A: Marker-assisted introgression of quantitative trait loci. Genetics 147: 1469–1485 (1997).
Jiang C, Zeng ZB: Multiple trait analysis of genetic mapping for quantitative trait loci. Genetics 140: 1111–1127 (1995).
Johnson GR, Mumm RH: Marker assisted maize breeding. In: Proceedings 51th Annual Corn and Sorghum Research Conference Chicago, IL, 11- 12 December 1996, pp. 75–84. American Seed Trade Association, Washington, DC (1996).
Khavkin E, Coe E: Mapped genomic locations for developmental functions and QTLs reflect concerted groups in maize (Zea mays L.). Theor Appl Genet 95: 343–352 (1997).
Lander ES, Botstein D: Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121: 185–199 (1989).
Lark KG, Chase K, Adler F, Mansur LM, Orf JH: Interactions between quantitative trait loci in soybean in which trait variation at one locus is conditional upon a specific allele at another. Proc Natl Acad Sci USA 92: 4656–4660 (1995).
Lee M: DNA markers and plant breeding programs. Adv Agron 55: 265–344 (1995).
Melchinger AE, Boppenmaier J, Dhillon BS, Pollmer WG, Herrmann RG: Genetic diversity for RFLPs in European maize inbreds: II. Relation to performance of hybrids within vs. between heterotic groups for forage traits. Theor Appl Genet 84: 672–681 (1992).
Mohan M, Nair S, Bhagwat A, Krishna TG, Yano M, Bhatia CR, Sasaki T: Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol Breed 3: 87–103 (1997).
Moreno-Gonzalez J: Molecular markers and heterosis. In: Proceedings International Symposium on the Heterosis in Crops, 17- 22 August 1997, pp. 172–173. ASA, Mexico D.F.,Mexico (1997).
Openshaw SJ, Jarboe SG, Beavis WD: Marker-assisted selection in backcross breeding. In: Proceedings Symposium of the Analysis of Molecular Marker Data, 5- 6 August 1994, pp. 41–43. Crop Science Society of America, Corvallis, OR (1994).
Penner GA, Lee SJ, Bezte LJ, Ugali E: Rapid RAPD screening of plant DNA using dot blot hybridization. Mol Breed 2: 7–10 (1996).
Powell W, Machray GC, Provan J: Polymorphism revealed by simple sequence repeats. Trends Plant Sci 1: 215–221 (1996).
Ragot M, Biasiolli M, Delbut MF, Dell'orco A, Malgarini L, Thevenin P, Vernoy J, Vivant J, Zimmermann R, Gay G: Marker-assisted backcrossing: a practical example. In: Techniques et utilisations des marqueurs moléculaires, pp. 45–56. Les Colloques, No. 72. INRA, Paris (1995).
Ribaut J-M, Jiang C, Gonzalez-de-Leon D, Edmeades GO, Hoisington DA: Identification of quantitative trait loci under drought conditions in tropical maize. 2. Yield components and marker-assisted selection strategies. Theor Appl Genet 94: 887–896 (1997).
Ribaut J-M, Hu X, Hoisington DA, Gonzalez-de-Leon D: Use of STSs and SSRs as rapid and reliable preselection tools in a marker assisted selection backcross scheme. Plant Mol Biol Rep 15: 154–162 (1997).
Ribaut J-M, Betran FJ: Plant improvement through molecular markers: a new approach. In: Proceedings International Symposium on the Heterosis in Crops, 17- 22 August 1997, pp. 32–33. ASA, Mexico D. F., Mexico (1997).
Ribaut J-M, Hoisington DA: Marker-assisted selection: new tools and strategies. Trends Plant Sci 3: 236–239 (1998).
Talbert LE, Blake NK, Chee PW, Blake TK, Magyar GM: Evaluation of ‘sequence-tagged-site’ PCR products as molecular markers in wheat. Theor Appl Genet 87: 789–794 (1994).
Tanksley SD: Mapping polygenes. Annu Rev Genet 27: 205–233 (1993).
Tanksley SD, McCouch SR: Seed banks and molecular maps: unlocking genetic potential from the wild. Science 277: 1063–1066 (1997).
Tanksley SD, Nelson JC: Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet 92: 191–203 (1996).
Zeng ZB: Precision mapping of quantitative trait loci. Genetics 136: 1457–1468 (1994).
About this article
Cite this article
Ribaut, JM., Betrán, J. Single large-scale marker-assisted selection (SLS-MAS). Molecular Breeding 5, 531–541 (1999). https://doi.org/10.1023/A:1009631718036
- crop improvement
- marker-assisted selection
- quantitative trait loci
- Zea mays L.