Skip to main content
SpringerLink
Log in

A molecular marker associated with the H21 Hessian fly resistance gene in wheat

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Near-isogenic lines in conjunction with bulked segregant analysis were used to identify a DNA marker in wheat (Triticum aestivum L.) associated with the H21 gene conferring resistance to biotype L of Hessian fly [Mayetiola destructor (Say)] larvae. Near-isogenic lines were developed by backcross introgression BC3F3:4 (‘Coker 797’ * 4 / ‘Hamlet’) and differed by the presence or absence of H21 (on 2RL) derived from ‘Chaupon’ rye (Secale cereale L.). Bulked DNA samples were prepared from near-isogenic lines and BC3F2 population individuals segregating for reaction to Hessian fly biotype L and screened for random amplified polymorphic DNA markers using 46 10mer primers. Random-amplified polymorphic DNA markers from resistant and susceptible individuals and parental lines were scored and these data were used to identify a 3 kb DNA fragment that was related to the occurrence of H21. This fragment was amplified from DNA isolated from Hamlet, a near-isogenic line carrying 2RL, and bulked DNA from resistant BC3F2 individuals, but not from the recurrent parent Coker 797 or DNA bulks from susceptible BC3F2 plants. Analysis of 111 BC3F2 segregating individuals and BC3F2:3 segregants confirmed the co-segregation of the 3 kb DNA marker with the H21 resistance gene to Hessian fly. Use of this marker could facilitate more rapid screening of plant populations for Hessian fly resistance and monitoring the introgression of H21.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adam-Blondon AF, Sévignac M, Bannerot H, Dron M: SCAR, RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose in common bean. Theor Appl Genet 88: 865–870 (1994).

    Google Scholar 

  2. Cartwright WB, LaHue DW: Testing wheats in the greenhouse for Hessian fly resistance. J Econ Entomol 37: 385–387 (1944).

    Google Scholar 

  3. Carver BF, Rayburn AL, Hunger RM, Smith EL, Whitmore WE: Registration of 1B versus 1RS.1BL near-isoline genetic stock from two hard red winter wheat populations. Crop Sci 33: 1120 (1993).

    Google Scholar 

  4. Delaney DE, Friebe BR, Hatchett JH, Gill BS, Hulbert SH: Targeted mapping of rye chromatin in wheat by representational difference analysis. Genome 38: 458–466 (1995).

    Google Scholar 

  5. Devos KM, Millan T, Gale MD: Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley. Theor Appl Genet 85: 784–792 (1993).

    Google Scholar 

  6. Dweikat I, Ohm H, Mackenzie S, Patterson F, Cambron S, Ratcliffe R: Association of a DNA marker with Hessian fly resistance gene H9 in wheat. Theor Appl Genet 89: 964–968 (1994).

    Article  Google Scholar 

  7. Friebe B, Hatchett JH, Sears RG, Gill BS: Transfer of hessian fly resistance from ‘Chaupon’ rye to hexaploid wheat via a 2BS/2RL wheat-rye chromosome translocation. Theor Appl Genet 79: 385–389 (1990).

    Google Scholar 

  8. Friebe B, Hatchett JH, Gill BS, Mukai Y, Sebesta EE: Transfer of Hessian fly resistance from rye to wheat via radiationinduced terminal and intercalary chromosomal translocation. Theor Appl Genet 83: 33–40 (1991).

    Article  Google Scholar 

  9. Haley SD, Miklas PN, Stavely JR, Byrum J, Kelly JD: Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theor Appl Genet 86: 505–512 (1993).

    Google Scholar 

  10. Haley SD, Afanador LK, Miklas PN, Stavely JR, Kelly JD: Heterogeneous inbred populations are useful as sources of near-isogenic lines for RAPD marker localization. Theor Appl Genet 88: 337–342 (1994).

    Article  Google Scholar 

  11. Hartl L, Weiss H, Stephan U, Zeller FJ, Jahoor A: Molecular identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). Theor Appl Genet 90: 601–606 (1995).

    Article  Google Scholar 

  12. Hatchett JH, Sears RG, Cox TS: Inheritance of resistance to Hessian fly in rye and in wheat-rye translocation lines. Crop Sci 33: 730–734 (1993).

    Google Scholar 

  13. Iqbal MJ, Rayburn AL: Identification of the 1RS rye chromosomal segment in wheat by RAPD analysis. Theor Appl Genet 91: 1048–1053 (1995).

    Article  Google Scholar 

  14. Johnson E, Miklas PN, Stavely JR, Martinez-Cruzado JC: Coupling-and repulsion-phase RAPDs for marker-assisted selection of PI 181996 rust resistance in common bean. Theor Appl Genet 90: 659–664 (1995).

    Article  Google Scholar 

  15. Knackstedt MA, Sears RG, Rogers DE, Lookhart GL: Effect of T2BS.2RL wheat-rye translocation on breadmaking quality in wheat. Crop Sci 34: 1066–1070 (1994).

    Google Scholar 

  16. Koebner RMD, Shepherd KW: Induction of recombination between rye chromosome 1RL andwheat chromosomes. Theor Appl Genet 71: 208–215 (1985).

    Google Scholar 

  17. Koebner RMD, Shepherd KW: Controlled introgression to wheat of genes from rye chromosome arm 1RS by induction of allosyndesis. 1. Isolation of recombinants. Theor Appl Genet 73: 197–208 (1986).

    Article  Google Scholar 

  18. Lee JH, Graybosch RA, Kaeppler SM, Sears RG: A PCR assay for detection of a 2RL.2BS wheat-rye chromosome translocation. Genome 39: 605–608 (1996).

    PubMed  Google Scholar 

  19. Ma Z-Q, Gill BS, Sorrells ME, Tanksley SD: RFLP markers linked to two Hessian fly-resistance genes in wheat (Triticum aestivum L.) from Triticum tauschii (Coss.) Schmal. Theor Appl Genet 85: 750–754 (1993).

    Article  Google Scholar 

  20. Martin GB, Williams JGK, Tanksley SD: Rapid identification of markers linked to a Pseudomonas resistance genes in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88: 2336–2340 (1991).

    PubMed  Google Scholar 

  21. Michelmore RW, Paran I, Kesseli RV: Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828–9832 (1991).

    PubMed  Google Scholar 

  22. Miklas PN, Stavely JR, Kelly JD: Identification and potential use of a molecular marker for rust resistance in common bean. Theor Appl Genet 85: 745–749 (1993).

    Article  Google Scholar 

  23. Ohmori T, Murata M, Motoyoshi F: Identification of RAPD markers linked to the Tm-2 locus in tomato. Theor Appl Genet 90: 307–311 (1995).

    Google Scholar 

  24. Patterson FL, Foster JE, Ohm HW, Hatchett JH, Taylor PL: Proposed system of nomenclature for biotypes of Hessian fly (Diptera: Cecidomyiidae) in North America. J Econ Entomol 85: 307–311 (1992).

    Google Scholar 

  25. Penner GA, Chong J, Lévesque-Lemay M, Molnar SJ, Fedak G: Identification of a RAPD marker linked to the oat stem rust gene Pg3. Theor Appl Genet 85: 702–705 (1993).

    Google Scholar 

  26. Raupp WJ, Amri A, Hatchett JH, Gill BS, Wilson DL, Cox TS: Chromosomal location of Hessian fly resistance genes H22, H23, and H24 derived from Triticum tauschii in the D genome of wheat. J Hered 84: 142–145 (1993).

    Google Scholar 

  27. Schachermayr G, Siedler H, Gale MD, Winzeler H, Winzeler M, Keller B: Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor Appl Genet 88: 110–115 (1994).

    Google Scholar 

  28. Sears RG, Hatchett JH, Cox TS, Gill BS: Registration of hamlet, a Hessian fly resistant hard red winter wheat germplasm. Crop Sci 32: 506 (1992).

    Google Scholar 

  29. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 18: 6531–6535 (1990).

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Y. W. Seo

    Present address: Department of Agronym, Korea University, Anam-Dong, Seongbuk-Gu, Seoul, 136-701, Korea

Authors and Affiliations

  1. Department of Crop and Soil Science, University of Georgia-Griffinm, 1109 Experiment Street, Griffin, GA, 30223

    Y. W. Seo

  2. Department of Crop and Soil Science, University of Georgia-Griffinm, 1109 Experiment Street, Griffin, GA, 30223

    J. W. Johnson

  3. Genetic Resources Unit, Georgia Experiment Station, USDA/ARS, 1109 Experiment Street, Griffin, GA, 30223, USA

    R. L. Jarret

Authors
  1. Y. W. Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. W. Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. L. Jarret
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Seo, Y.W., Johnson, J.W. & Jarret, R.L. A molecular marker associated with the H21 Hessian fly resistance gene in wheat. Molecular Breeding 3, 177–181 (1997). https://doi.org/10.1023/A:1009606304447

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1009606304447

Search

Navigation