Abstract
The agronomically important Indica (group 1) rice varieties IR64, IR72, hybrid restorer line Minghui 63, and BG90-2 were co-transformed by microbombardment of embryogenic suspensions with plasmids that contain the Xa21 gene which confers resistance to Xanthomonas oryzae pv. oryzae and the hph gene for resistance to hygromycin B. Six of the 55 transgenic R0 plant lines containing the Xa21 gene displayed high levels of resistance to the pathogen, and no partial resistance was observed. The trait was stably inherited in subsequent generations, and transgenic plants are currently in field tests. The ability to transfer agronomically important genes into elite Indica rice varieties demonstrates the applicability of genetic engineering for the agronomic improvement of rice.
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Chen L, Marmey P, Taylor N, Brizard JP, Espinoza C, D'Cruz P, Huet H, Zhang S, de Kochko A, Beachy RN, Fauquet CM: Integration, expression and inheritance of multiple transgenes in rice plants. Nature Biotechnology, in press (1998).
Chen L, Zhang S, Beachy R, Fauquet C: A protocol for consistent, large scale production of fertile transgenic rice plants. Plant Cell Rep, in press (1998).
Christou P, Ford TL, Kofron M: Production of transgenic rice (Oryza sativa L.) plants from agronomically important Indica and Japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/technology 9: 957–962 (1991).
David CC: The world rice economy: challenges ahead. In: Khush GS, Toenniessen GH (eds) Rice Biotechnology, pp. 1–18. CAB International, Wallingford, UK (1991).
Dellaporta SL, Wood J, Hicks JB: A plant DNA minipreparation: Version II. Plant Mol Biol Rep 1: 19–21 (1983).
Fujimoto H, Itoh K, Yamamoto M, Kyozuka J, Shimamoto, K: Insect resistant rice generated by introduction of a modified ∂-endotoxin gene of Bacillus thuringiensis.Bio/technology 11: 1151–1155 (1993).
Gamborg O, Miller R, Ojlma K: Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158 (1968).
Glaszmann JC: Isozyme and classification of Asian rice varieties. Theo Appl Genet 74: 21–30 (1987).
Hayakawa T, Zhu Y, Itoh K, Kimura Y, Izawa T, Shimamoto K, Toriyama S: Genetically engineered rice resistant to rice strip virus, an insect-transmitted virus. Proc Natl Acad Sci USA 89: 9865–9869 (1992).
Herdt RW: Research priorities for rice biotechnology. In: Khush GS, Toenniessen GH (eds) Rice Biotechnology, pp. 19–54. CAB International, Wallingford, UK (1991).
Kauffman HE, Reddy APK, Hsieh SPV, Marca SD: An improved technique for evaluation of resistance of rice varieties to Xanthomonas oryzae.Plant Dis Rep 57: 537–541 (1973).
Khush GS, Bacalangco E, Ogawa T: A new gene for resistance to bacterial blight from O. longistaminata.Rice Genet Newsl 17; 121–122 (1990).
Krattiger AF: The role of the private sector in biotechnology transfer to developing countries. Agricultural Biotechnology International Conference, Saskatoon, pp. 143–144 (1996).
Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497 (1962).
Ohira K, Ojima K, Fijiwara A: Studies on the nutrition of rice cell culture. I. A simple, defined medium for rapid growth in suspension culture. Plant Cell Physiol 14: 1113–1121 (1973).
Potrykus I, Harms CT, Lörz H: Callus formation from cell culture protoplasts of corn (Zea mays L.). Theor Appl Genet 54: 209–214 (1979).
Ronald P: Making rice disease-resistant. Scientific American, November (1997).
Ronald P, Albano B, Tabien R, Abenes L, Wu K. McCouch S, Tanksley S: Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21. Mol Gen Genet 236: 113–120 (1992).
Song WY, Wang G, Chen L, Kim H, Pi LY, Holsten T, Gardner J, Wang B, Zha W, Zhu L, Fauquet C, Ronald P: A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science 270: 1804–1806 (1995).
Toenniessen GH: Potentially useful genes for rice genetic engineering. In: Khush GS, Toenniessen GH (eds) Rice Biotechnology, pp. 253–280. CAB International, Wallingford, UK (1991).
Wang Gl, Song WY, Ruan DL, Sideris S, Ronald P: The cloned gene, Xa21, confers resistance to multiple Xanthomonas oryzae pv. oryzae isolates in transgenic plants. Mol Plant-Microbe Interact 9: 850–855 (1996).
Wünn J, Klöti A, Burkhardt PK, Ghosh Biswas GC, Launis K, Iglesias VA, Potrykus I: Transgenic Indica rice breeding line IR58 expressing a synthetic cryIA(b) gene from Bacillus thuringiensis provides effective insect pest control. Bio/technology 14: 171–176 (1996).
Zhang S: Efficient plant regeneration from protoplasts of four true Indica (group 1) rice varieties and advanced line. Plant Cell Rep 15: 68–71 (1995).
Zhang S, Chen L, Qu R, Marmey P, Beachy R, Fauquet C: Regeneration of fertile transgenic Indica (group 1) rice plants following microprojectile transformation of embryogenic suspension culture cells. Plant Cell Rep 15: 465–469 (1996).
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Zhang, S., Song, WY., Chen, L. et al. Transgenic elite Indica rice varieties, resistant to Xanthomonas oryzae pv. oryzae. Molecular Breeding 4, 551–558 (1998). https://doi.org/10.1023/A:1009601520901
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DOI: https://doi.org/10.1023/A:1009601520901