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Isolation and General Characterization of Polysaccharides from Tansy Tanacetum vulgare L.


Using extraction with 0.75% aqueous ammonium oxalate, the following polysaccharide fractions were isolated: tanacetans TVF, TVS, and TVR from floscules, sprouts, and roots, respectively, of Tanacetum vulgare L., spread throughout the European North of Russia. The sugar chain of tanacetan TVF consists of D-galacturonic acid (61.4%), arabinose (14.7%), galactose (10.2%), and rhamnose (3.7%) as the main constituents as well as xylose, glucose, mannose, apiose, and 2-O-methylxylose in trace amounts. Tanacetans TVS and TVR were shown to differ in the sugar quantitative composition. They contain 67 and 28% galacturonic acid, respectively. A partial acid hydrolysis of the tanacetan TVF gave a polysaccharide fragment TVF1, α-1,4-D-galacturonan (GalA 98.2%). Digestion with pectinase (α-1,4-D-polygalacturonase) resulted in fragment TVF3, containing residues of arabinose (27.1%) and galactose (17.3%). NMR spectroscopy allowed detection of the terminal residues of α-Araf and β-Galp as well as of the residues of α-Arap substituted in 3,5- and 5-positions. Thus, tanacetan TVF was proved to be a pectic polysaccharide.

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  1. Abad, M.J., Bermejo, P., and Villar, A., Phytother. Res., 1995, vol. 9, pp. 79-92.

    Google Scholar 

  2. Minaeva, V.G., Lekarstvennye rasteniya Sibiri (Medicinal Plants of Siberia), Novosibirsk: Nauka, 1970, pp. 157-158.

    Google Scholar 

  3. Sysoeva, K.N., Gorin, A.G., and Yakovlev, A.I., Rast. Resursy, 1979, vol. 15, pp. 89-91.

    Google Scholar 

  4. Polle, A.Ya., Ovodova, R.G., and Popov, S.V., Khim. Rast. Syr'ya, 1999, no. 1, pp. 33-38.

  5. Ovodova, R.G., Vaskovsky, V.E., and Ovodov, Yu.S., Carbohydr. Res., 1968, vol. 6, pp. 328-332.

    Google Scholar 

  6. Perepelov, A.V., Babicka, D., Shashkov, A.S., Arbatsky, N.P., Senchenkova, S.N., Rozalski, A., and Knirel, Y.A., Carbohydr. Res., 1999, vol. 318, pp. 186-192.

    Google Scholar 

  7. Odonmäzig, P., Badga, D., Ebringerova, A., and Alföldi, J., Carbohydr. Res., 1992, vol. 226, pp. 353-358.

    Google Scholar 

  8. Pressey, R. and Himmelsbach, D.S., Carbohydr. Res., 1984, vol. 127, pp. 356-359.

    Google Scholar 

  9. Usov, A.I., Bilan, M.I., and Klochkova, N.G., Bot. Marina, 1995, vol. 38, pp. 43-51.

    Google Scholar 

  10. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., J. Biol. Chem., 1951, vol. 193, pp. 265-275.

    Google Scholar 

  11. York, W.S., Darvill, A.G., McNeil, M.A., Stevenson, T.T., and Albersheim, P., Meth. Enzymol., 1985, vol. 118, pp. 3-40.

    Google Scholar 

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Polle, A.Y., Ovodova, R., Shashkov, A.S. et al. Isolation and General Characterization of Polysaccharides from Tansy Tanacetum vulgare L.. Russian Journal of Bioorganic Chemistry 27, 45–49 (2001).

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  • polysaccharide
  • tansy Tanacetum vulgare L.
  • pectins
  • arabinogalactans
  • tanacetans
  • NMR spectroscopy of polysaccharides