Skip to main content

Study of the In Vitro Maturation of Mouse Oocytes Induced by Microinjection of Maturation Promoting Factor (MPF)

Journal of Assisted Reproduction and Genetics volume 17, pages 67–73 (2000)Cite this article

Abstract

Purpose: Maturation promoting factor (MPF) acts at theresumption of meiosis and nonspecifically throughout theanimal species. There exists a considerable body of literatureon MPF, but little work has been done to study the inductionof maturation of mammalian oocytes by microinjection ofextracted MPF.

Methods: Immature (GV-stage) mouse oocytes weremicroinjected MPF extracted from matured Xenopus eggs in thepresense of dbcAMP.

Results: The rate of germinal vesicle breakdown (GVBD)induced at 24 hr after MPF injection was significantly higher(90.5%) than that of the control (2.2%), which was injectedwith HTF medium containing dbcAMP (P < 0.0001). Therate of extrusion of the first polar body at 24 hr after MPFinjection was significantly higher (84.1%) than that of thesame control (1.1%) (P < 0.0001).

Conclusions: From these results, it is concluded that thematuration of mammalian oocytes can be induced by themicroinjection of MPF extracted from other species.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

REFERENCES

  1. Masui Y, Markert CL: Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes. J Exp Zool 1971;177:129-146

    Google Scholar 

  2. Kishimoto T: Regulation of metaphase by a maturation-promoting factor. Dev Growth Different 1988:30:105-115

    Google Scholar 

  3. Hashimoto N, Kishimoto T: Regulation of miotic metaphse by a cytoplasmic maturation-promoting factor during mouse oocyte maturation. Dev Biol 1988; 126:242-252

    Google Scholar 

  4. Sorensen RA, Cyert MS, Pedersen RA: Active maturation-promoting factor is present in mature mouse oocytes. J Cell Biol 1985;100:1637-1640

    Google Scholar 

  5. Miake-Lye R, Newport J, Kirschner M: Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis. J Cell Biol 1983;97:81-91

    Google Scholar 

  6. Balakier H, Czolowska R: Cytoplasmic control of nuclear maturation in mouse oocytes. Exp Cell Res 1977;110:466-469

    Google Scholar 

  7. Fulka J Jr: Nuclear maturation in pig and rabbit oocytes after interspecific fusion. Exp Cell Res 1983;146:212-218

    Google Scholar 

  8. Prochazka R, Motlic J, Fulka J: Activity of maturation promoting factor in pig oocytes after microinjection and serial transfer of maturing cytoplasm. Cell Different Dev 1989;27:175-182

    Google Scholar 

  9. Kishimoto T, Yamazaki K, Kato Y, et al.: Induction of starfish oocyte maturation by maturation-prommoting factor of mouse and surf clam oocytes. J Exp Zool 1984;231:293-295

    Google Scholar 

  10. Sunkara PS, Wright DA, Rao PN: Mitotic factors from mammalian cells induce germinal vesicle breakdown and chromosome condensation in amphibian oocytes. Proc Natl Acad Sci USA 1979;76:2799-2802

    Google Scholar 

  11. Lohka MJ, Hayes MK, Maller JL: Purification of maturation-promoting factor, an intracellular regulator of early mitotic events. Proc Natl Acad Sci USA 1988;85:3009-3013

    Google Scholar 

  12. Yamashita S, Maller JL: Identification of an activator required for elevation of maturation-promoting factor(MPF) activity by ?-s-ATP. J Cell Biol 1990;110:1583-1588

    Google Scholar 

  13. Kimura Y, Yanagimachi R: Intracytoplasmic sperm injection in the mouse. Biol Reprod 1995; 52:709-720

    Google Scholar 

  14. Van deWiel DFM, Bar-Ami S, Tsafriri A, et al.: Oocyte maturation inhibitor, inhibin and steroid concentrations in porcine follicular fluid at various stages of the estrous cycle. J Reprod Fertil 1983;68:247-252

    Google Scholar 

  15. Cho WK, Stern S, Biggers JD: Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro. J Exp Zool 1974;187:383-386

    Google Scholar 

  16. Lindner HR, Tsafriri A, Lieberman ME, et al.: Gonadotropin action on cultured Graafian follicles: Induction of maturation division of the mammalian oocyte and differentiation of the luteal cell. Recent Prog Horm Res 1974;30:79-138

    Google Scholar 

  17. Dekel N, Galiani D, Sherizly I: Dissociation between the inhibitory and the stimulatory action of cAMP on maturation of rat oocytes. Mol Cell Endocrinol 1988;56:115-121

    Google Scholar 

  18. Cross PC: The role of cumulus cells and serum in mouse oocyte maturation in vitro. J Reprod Fertil 1973;34:241-245

    Google Scholar 

  19. Gavin AC, Vassalli JD, Cavadore JC, Schorderet-Slatkine S: Okadaic acid and p13suc1 modulate the reinitiation of meiosis in mouse oocytes. Mol Reprod Dev 1992;33:287-296

    Google Scholar 

Download references

Author information

Affiliations

  1. First Department of Obstetrics and Gynecology, Toho University School of Medicine, 6-11-1, Ohmori-nishi, Ohta Word, Tokyo, 143, Japan

    Hideyuki Nakano & Harumi Kubo

Authors
  1. Hideyuki Nakano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Harumi Kubo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Nakano, H., Kubo, H. Study of the In Vitro Maturation of Mouse Oocytes Induced by Microinjection of Maturation Promoting Factor (MPF). J Assist Reprod Genet 17, 67–73 (2000). https://doi.org/10.1023/A:1009458215882

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1009458215882

  • maturation promoting factor
  • immature oocyte
  • in vitro maturation
  • microinjection