Abstract
The iron-bleomycin complex has been shown to catalyze the oxidation of p-phenylenediamine to a stable, purple coloured oxidation product, characterized by an absorption maximum around 520 nm. Molecular oxygen is used for reoxidizing Fe(II)-bleomycin after reduction by p-phenylenediamine. An apparent Michaelis constant of 5.2 mM and a catalytic constant of 17.2 min−1 were obtained from kinetic studies. ATP, ADP and orthophosphate inhibited the catalytic oxidation of p-phenylenediamine, while AMP was without effect. It is proposed that p-phenylenediamine may be used as ‘substrate’ in kinetic studies involving the ‘oxidase’ activity of iron-bleomycin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Buettner GR, Moseley PL. 1992 Ascorbate both activates and inactivates bleomycin by free radical generation. Biochemistry 31, 9784-9788.
Burger RM, Peisach J, Blumberg WE, Horwitz SB. 1979 Ironbleomycin interactions with oxygen and oxygen analogues. J Biol Chem 21, 10906-10912.
Burger RM, Peisach J, Horwitz SB. 1981 Activated bleomycin. A transient complex of drug, iron, and oxygen that degrades DNA. J Biol Chem 25, 11636-11644.
Burger RM, Horwitz SB, Peisach J. 1985 Stimulation of iron (II) bleomycin activity by phosphate-containing compounds. Biochemistry 24, 3623-3629.
Caspary WJ, Niziak C, Lanzo DA, Friedman R, Bachur NR. 1979 Bleomycin A2: A ferrous oxidase. Mol Pharmacol 16, 256-260.
Holmberg CG, Laurell C-B. 1951 Investigations in serum copper III. Coeruloplasmin as an enzyme. Acta Chem Scand 5, 476-480.
Lown JW, Joshua AV, Chen H-H. 1982 Reactive oxygen species leading to lipid peroxidation and DNA lesions implicated in the cytotoxic action of certain antitumour antibiotics. In: McBrien DCH, Slater TF, eds. Free Radicals, Lipid Peroxidation and Cancer. London: Academic Press; 305-320.
Peisach J, Levine WG. 1963 On the mechanism of ceruloplasmin-catalyzed oxidations. Biochim Biophys Acta 77, 615-628.
Rice EW. 1962 Standardization of ceruloplasmin activity in terms of international enzyme units. Anal Biochem 3, 452-456.
Sausville EA, Peisach J, Horwitz SB 1976. A role for ferrous ion and oxygen in the degradation of DNA by bleomycin. Biochem Biophys Res Commun 73, 814-822.
Sausville EA, Stein RW, Peisach J, Horwitz SB 1978. Effect of chelating agents and metal ions on the degradation of DNA by bleomycin and Fe(II). Biochemistry 17, 2746-2754.
Shields H, McGlumphy C. 1984 The orientation of the ligands in Fe(III)-bleomycin intercalated with DNA. Biochim Biophys Acta 800, 277-281.
Suzuki H, Nagai K, Yamaki H, Tanaka N, Umezawa H. 1969. On the mechanism of action of bleomycin: Scission of DNA strands in vitro and in vivo. J Antibiot 22, 446-448.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Løvstad, R.A. Interaction of p-phenylenediamine with the iron-bleomycin complex. Biometals 12, 233–236 (1999). https://doi.org/10.1023/A:1009298914400
Issue Date:
DOI: https://doi.org/10.1023/A:1009298914400