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Chromosome Research

, Volume 8, Issue 3, pp 193–199 | Cite as

Chromosomal Location and Nucleotide Sequences of 5S Ribosomal DNA of Two Cyprinid Species (Osteichthyes, Pisces)

  • Junya Inafuku
  • Mika Nabeyama
  • Yutaka Kikuma
  • Junji Saitoh
  • Souichirou Kubota
  • Sei-ichi Kohno
Article

Abstract

5S ribosomal DNAs (rDNAs) from two cyprinid species, Acheilognathus tabira subsp. 1 and Cyprinus carpio, were isolated and sequenced. Tandemly arranged rDNAs were 179 bp in A. tabira and 204 bp in C. carpio. The non-transcribed spacer region elucidates the size difference of 5S rDNA between the two species. Fluorescence in-situ hybridization (FISH) localized 5S rDNAs to the short arms of two pairs of chromosomes in A. tabira and two to four pairs in C. carpio. Subsequent analysis demonstrated NORs in one pair of chromosomes in both species. Both the NOR and 5S rDNA are carried by a chromosome pair in A. tabira, but they are located on different chromosomes separately in C. carpio. Karyotype evolution by tetraploidy seems complex in cyprinid species.

cyprinid fishes fluorescence in-situ hybridization karyotype evolution NOR 5S ribsomal DNAs 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Junya Inafuku
    • 1
  • Mika Nabeyama
    • 1
  • Yutaka Kikuma
    • 1
  • Junji Saitoh
    • 1
  • Souichirou Kubota
    • 1
  • Sei-ichi Kohno
    • 1
  1. 1.Department of Biology, Faculty of ScienceToho UniversityChibaJapan

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