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Synteny and Regional Marker Order Sssignment of 26 type I and Microsatellite Markers to the Horse X- and Y-Chromosomes

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Abstract

The hypothesis that the conservation of sex-chromosome-linked genes among placental mammals could be extended to the horse genome was tested using the UCDavis horse–mouse somatic cell hybrid (SCH) panel. By exploiting the fluorescence in-situ hybridization (FISH) technique to localize an anchor locus, X-inactivation-specific transcript (XIST) on the horse X chromosome, together with the fragmentation and translocation of the X- and Y-chromosome fragments in a somatic cell hybrid panel, we regionally assigned 13 type I and 13 type II (microsatellite) markers to the horse X- and Y-chromosomes. The synteny groups that correspond to horse X- and Y-chromosomes were identified by synteny mapping of sex-specific loci zinc finger protein X-linked (ZFX), zinc finger protein Y-linked (ZFY) and sex-determining region Y (SRY) on the SCH panel. A non-pseudoautosomal gene in the human steroid sulfatase (STS) was identified in both X- and Y-chromosome- containing clones. The regional order of the X-linked type I markers examined in this study, from Xp- to Xq-distal, was [STS- X, the voltage-gated chloride channel 4 (CLCN4)], [ZFX, delta- aminolevulinate synthase 2 (ALAS2)], XIST, coagulation factor IX (F9) and [biglycan (BGN), equine F18, glucose-6-phosphate dehydrogenase (G6PD)] (precise marker order could not be determined for genes within the same brackets). The order of the Y-linked type I markers was STS-Y, SRYand ZFY. These orders are the same arrangements as reported for the human X- and Y-chromosomes, supporting the conservation of genomic organization between the human and the horse sex chromosomes. Regional ordering of X- linked type I and microsatellite markers provides the first integration of type I and type II markers in the horse X chromosome.

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Authors and Affiliations

  1. Veterinary Genetics Laboratory, School of Veterinary Medicine, University of California, Davis, CA, 95616-8744, USA

    Y.-L. Shiue, L. V. Millon & A. T. Bowling

  2. Department of Veterinary Anatomy, Texas A&M University, College Station, TX, 77843-4458, USA

    L. C. Skow & D. Honeycutt

  3. Department of Animal Science, University of California, Davis, CA, 95616, USA

    J. D. Murray

  4. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA

    J. D. Murray

  5. Reproduction, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA

    A. T. Bowling

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  1. Y.-L. Shiue
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  2. L. V. Millon
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  3. L. C. Skow
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  4. D. Honeycutt
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  5. J. D. Murray
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  6. A. T. Bowling
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Shiue, YL., Millon, L.V., Skow, L.C. et al. Synteny and Regional Marker Order Sssignment of 26 type I and Microsatellite Markers to the Horse X- and Y-Chromosomes. Chromosome Res 8, 45–55 (2000). https://doi.org/10.1023/A:1009275102977

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