Abstract
The dioecious plant Rumex acetosa has a multiple sex chromosome system: females are 2n = XX + 12, males are 2n = XY1Y2 + 12, and the two Y chromosomes are heterochromatic. A DNA sequence abounded in the male genome was isolated and analyzed. The sequence (RAE180) was a 180-bp-long tandemly arranged repetitive sequence, distributed in chromosomes Y1 and Y2, and two pairs of autosomes. Both Y chromosomes contained large amounts of RAE180 and the sequence formed many DAPI bands, while, on the two pairs of autosomes, RAE180 did not form DAPI bands. The internal structure and morphological changes of the Y chromosomes were analyzed by FISH, using RAE180 and the Y-chromosome-specific sequence RAYSI as probes. The pattern of the FISH signals caused by the accumulation of RAE180 and RAYSI suggested the structural change in the Y chromosomes during the process of sex chromosome evolution, and the morphological change in the Y chromosomes was explained by reciprocal translocation and inversion.
Similar content being viewed by others
References
Ainsworth CC, Lu J, Winfield M, Parker J (1999) Sex determination by X: autosome dosage: Rumex acetosa (sorrel). In: Sex Determination in Plants. UK: Bios Scientific Publishers, pp 121–136.
Hizume M (1992) Exact location of rRNA genes in Vicia faba chromosomes. Cytologia 57: 471–475.
Kihara H, Ono T (1923) Cytological studies on Rumex L. I. Chromosomes of Rumex acetosa L. Bot Mag Tokyo 37: 84–90.
Kurita M, Kuroki Y (1970) Y-chromosome and heterochromatin in Rumex acetosa. Jpn J Genet 45: 255–260.
Kuroki Y (1976) Studies on the karyotypes of Rumex acetosa. Mem Ehime Univ Ser B 8: 8–85.
Kuroki Y (1984) Fluorescence patterns of Rumex acetosa chromosomes. Chromosome Inf Service 37: 29–31.
Kuroki Y (1987) Karyotype variation and heterochromatin in Rumex acetosa. Plant Chromosome Res 1987, Hiroshima, Japan: Nishiki Print Co., Ltd., pp. 227–230.
Kuroki Y (1992) Cytological characteristics of sex chromosomes of Rumex acetosa. In: Chromosome Research 1992. Beijing, China: International Academic Publishers, pp 279–287.
Kuroki Y, Yokahama A, Iwatsubo Y (1994) Fluorescent chromosome banding in Rumex montanus (Polygonaceae). La Kromosomo II-74: 2591–2597.
Murray HG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321–4325.
Ruiz Rejón C, Jamilena M, Ramos MG, Parker JS, Rejón MR (1994) Cytologenetic and molecular analysis of the multiple sex chromosome system of Rumex acetosa. Heredity 72: 209–215.
Schweizer D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58: 307–324.
Shibata F, Hizume M, Kuroki Y (1999) Chromosome painting of Y chromosomes and isolation of a Y chromosome-specific repetitive sequence in the dioecious plant Rumex acetosa. Chromosoma 108: 266–270.
Smith BW (1969) Evolution of sex-determining mechanisms in Rumex. Chromosomes Today 2: 172–182.
Váña V (1972) The localization of heterochromatic segments in the chromosomes of Rumex acetosa L. Preslia 44: 316–326.
Wilby AS, Parker JS (1986) Continuous variation in Y-chromosome structure of Rumex acetosa. Heredity 57: 247–254.
Wilby AS, Parker JS (1988) Recurrent patterns of chromosome variation in a species group. Heredity 61: 55–62.
Yonezawa Y, Tanaka R, Kuroki Y (1978) C-banding treatment of sex chromosomes in Rumex acetosa L. Chromosome Inf Service 25: 31–32.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shibata, F., Hizume, M. & Kuroki, Y. Differentiation and the Polymorphic Nature of the Y Chromosomes Revealed by Repetitive Sequences in the Dioecious Plant, Rumex Acetosa . Chromosome Res 8, 229–236 (2000). https://doi.org/10.1023/A:1009252913344
Issue Date:
DOI: https://doi.org/10.1023/A:1009252913344