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Chromosome Research

, Volume 8, Issue 3, pp 213–218 | Cite as

Major and 5S Ribosomal Sequences of the Largemouth Bass Micropterus Salmoides (Perciformes, Centrarchidae) are Localized in GC-Rich Regions of the Genome

  • Anna Maria Deiana
  • Angelo Cau
  • Susanna Salvadori
  • Elisabetta Coluccia
  • Rita Cannas
  • Angela Milia
  • James Tagliavini
Article

Abstract

Major and 5S ribosomal genes have been localized in the chromosomes of Micropterus salmoides. By C-banding, Ag-staining, CMA3-staining and 45S and 5S fluorescence in-situ hybridization (FISH), we demonstrate that the 45S and 5S ribosomal genes are clustered in two different chromosome pairs and both are located in heterochromatic GC-rich regions. PCR amplification and sequencing of the 5S intergenic non-transcribed sequences have allowed us to identify variability essentially due to a trinucleotide tandem repeat (GCT).

Ag-NOR chromomycin A3 banding DNA repeats FISH Micropterus salmoides ribosomal genes 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Anna Maria Deiana
    • 1
  • Angelo Cau
    • 1
  • Susanna Salvadori
    • 1
  • Elisabetta Coluccia
    • 1
  • Rita Cannas
    • 1
  • Angela Milia
    • 1
  • James Tagliavini
    • 2
  1. 1.Dipartimento di Biologia Animale ed EcologiaUniversità di CagliariCagliariItaly
  2. 2.Dipartimento di Biologia EvolutivaUniversità di ParmaParmaItaly

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