Abstract
Major and 5S ribosomal genes have been localized in the chromosomes of Micropterus salmoides. By C-banding, Ag-staining, CMA3-staining and 45S and 5S fluorescence in-situ hybridization (FISH), we demonstrate that the 45S and 5S ribosomal genes are clustered in two different chromosome pairs and both are located in heterochromatic GC-rich regions. PCR amplification and sequencing of the 5S intergenic non-transcribed sequences have allowed us to identify variability essentially due to a trinucleotide tandem repeat (GCT).
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Deiana, A.M., Cau, A., Salvadori, S. et al. Major and 5S Ribosomal Sequences of the Largemouth Bass Micropterus Salmoides (Perciformes, Centrarchidae) are Localized in GC-Rich Regions of the Genome. Chromosome Res 8, 213–218 (2000). https://doi.org/10.1023/A:1009248828365
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DOI: https://doi.org/10.1023/A:1009248828365