This paper reports on an experiment to determine growth and survival of blacklip pearl oyster, Pinctada margaritifera (L.), larvae fed a 1:1 mixture of Isochrysis aff. galbana clone T-ISO and Pavlova salina at six different densities (1, 2, 5, 10, 20 and 30 × 103 cells ml-1. Larval growth and survival were assessed every four days over a 20–day period. Exponential and logistic regression models were fitted to the growth and survival responses, respectively. Overall growth of larvae fed ≥5 × 103 cells ml-1 was significantly greater (p > 0.01) than growth of larvae reared at other algal densities. The optimal food ration for maximum larval growth was 20 × 103 cells ml-1, which resulted in larvae with antero-posterior shell length of 230 μm after 20 days. These larvae were significantly larger (p > 0.05) than those in all other treatments at the end of the experiment. Survival of larvae fed 0, 1 and 2 × 103 cells ml-1 was significantly lower than that of larvae in all other treatments at the end of 15 days (p > 0.01). Maximal survival (8%) over the 20 day period was shown by larvae fed 10 × 103 cells ml-1, while lower survival was shown by larvae fed 2 × 103 cells ml-1 (2%) and 1 × 103 cells ml-1 (0%).
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Alagarswami, K., Dharmaraj, S., Velayudhan, T.S., Chellam, A., Victor, A.C.C. and Gandhi, A.D. (1983). Larval rearing and production of spat of pearl oyster, Pinctada fucata (Gould). Aquaculture 34, 287–301.
Alagarswami, K., Dharmaraj, S., Chellam, A. and Velayudhan, T.S. (1989). Larval and juvenile rearing of black-lip pearl oyster, Pinctada margaritifera (Linnaeus). Aquaculture 76, 43–56.
Araya-Nunez, O., Ganning, B. and Buckle-Ramirez, F. (1995). Embryonic development, larval culture, and settling of American pearl oyster ( Pteria sterna, Gould) spat. California Fish and Game 81, 10–21.
Coeroli, M., De Gaillande, D., Landret, J.P. and Coatanea, D. (1984). Recent innovations in cultivation of molluscs in French Polynesia. Aquaculture 39, 45–67.
Gervis, M.H. and Sims, N.A. (1992). The biology and culture of pearl oysters (Bivalvia: Pteriidae). ICLARM Stud. Rev. 21. 49 pp.
Guillard R.L. (1983). Culture of phytoplankton for feeding marine invertebrates. In:, Culture of Marine Invertebrates, C.L. Berg (ed.), pp. 108–132. Hutchinson Ross Publishing Company, Stroudberg, PA.
Jespersen, H. and Oslen, K. (1982). Bioenergetics in veliger larvae of Mytilus edulis (L.). Ophelia 21, 101–113.
Loosanoff, V. L. and Davis, H. C. (1963). Rearing of bivalve mollusks. Advance in Marine Biology 61, 283–288.
Minaur, J. (1969). Experiments on the artificial rearing of the larvae of Pinctada maxima (Jameson) (Lamellibranchia). Australian Journal of Marine and Freshwater Research 20, 175–187.
Nell, J.A. and O'Connor, W.A. (1991). The evaluation of fresh algae and stored algal concentrates as a food source for Sydney rock oyster, Saccostrea commercialis (Iredale and Roughley), larvae. Aquaculture 99, 277–284.
Riisgard, H.U. (1988). Feeding rates in the hard clam (Mercenaria mercenaria) veliger larvae as a function of (Isochrysis galbana) algal concentration. Journal of Shellfish Research 7, 377–378.
Riisgard, H. U. (1991). Filtration rate and growth in the blue mussel, Mytilus edulis, Linnaeus, 1758: dependance on algal concentration. Journal of Shellfish Research 10, 29–35.
Rose, R.A. (1990). A manual for the artificial propagation of of the gold-lip or silver-lip pearl oyster, Pinctada maxima (Jameson) from Western Australia. Fisheries Department, Western Australian marine Research Laboratories, P.O. Box 20, North Beach WA 6020. Commonwealth FRDC, 41 pp.
Rose, R.A. and Baker, S.B. (1994). Larval and spat culture of the Western Australian silver-or gold-lip pearl oyster, Pinctada maxima (Jameson) (Mollusca: Pteriidae). Aquaculture 126, 35–50.
Southgate, P.C. and Beer, A.C. (1997). Hatchery and early nursery culture of the blacklip pearl oyster (Pinctada margaritifera, L.). Journal of Shellfish Research 16, 561–567.
Southgate, P.C. and Ito, M. (1998). Evaluation of a partial flow-through culture technique for pearl oyster (Pinctada margaritifera L.) larvae. Aquaculture Engineering 18, 1–7.
Southgate, P.C., Beer, A.C., Duncan P.F. and Tamburri R. (1998). Assessment of the nutritional value of three species of tropical microalgae, dried Tetraselmis and a yeast-based diet for larvae of the black-lip pearl oyster, Pinctada margaritifera (L.). Aquaculture 162, 247–257.
Sprung M. (1984). Physiological energetics of mussel larvae (Mytilus edulis). I. Shell growth and biomass. Marine Ecology Progress Series 17, 283–293.
Tanaka, Y., Inoha, S. and Kakazu, K. (1970). Studies on seed production of black-lip pearl oyster Pinctada margaritifera, in Okinawa — V. Rearing of the larvae. Bulletin of Tokai Region Fisheries Research Laboratory 63, 97–106.
Thompson, P.A., Montagnes, D.J.S., Shaw, B.A. and Harrison, P.J. (1994). The influence of three algal filtrates on the grazing rate of larval osters (Crassostrea gigas), determined by fluorescent microspheres. Aquaculture 119, 237–247.
Webb, K.L. and Chu, F.-L.E. (1983). Phytoplankton as a food source for bivalve larvae. In: Proceedings of the Second International Conference on Aquaculture Nutrition: Biochemical and Physiological Approaches to Shellfish Nutrition. October 27–29, 1981 Rehoboth Beach, Delaware. G.D. Pruder, C.J. Langdon and D. Conklin, (eds), pp. 272–291. World Mariculture Society, Special Publication No. 2, Louisiana State University, Baton Rouge, Louisiana, U.S.A.
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Doroudi, M.S., Southgate, P.C. & Mayer, R.J. Growth and Survival of Blackslip Pearl Oyster Larvae Fed Different Densities of Microalgae. Aquaculture International 7, 179–187 (1999). https://doi.org/10.1023/A:1009207810808